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Relaxation and positive socio-emotional states can amplify RespHRV, yet the underlying mechanism remains largely unknown. Here we identify a hypothalamus\u2013brainstem neuronal pathway in rodents through which oxytocin (OT) amplifies RespHRV during calming behavior. OT neurons from the caudal paraventricular nucleus in the hypothalamus regulate the activity of a subgroup of inhibitory neurons in the pre-B\u00f6tzinger complex, the brainstem nucleus that generates the inspiratory rhythm. Specifically, OT enhances the glycinergic input from OT-receptor-expressing neurons in the pre-B\u00f6tzinger complex to cardiac-innervating parasympathetic neurons in the nucleus ambiguus during inspiration. This leads to amplified respiratory modulation of parasympathetic activity to the heart, thereby enhancing RespHRV. We show that OT neurons participate in the restoration of RespHRV amplitude during recovery from stress in mice, indicating that OT acts centrally to regulate cardiac activity during a calming behavior.<\/jats:p>","DOI":"10.1038\/s41593-025-02074-2","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:03:28Z","timestamp":1760951008000},"page":"2247-2261","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Oxytocin modulates respiratory heart rate variability through a hypothalamus\u2013brainstem\u2013heart neuronal pathway"],"prefix":"10.1038","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1111-9416","authenticated-orcid":false,"given":"Julie","family":"Buron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambre","family":"Linossier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gestreau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Schaller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Tyzio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Solenne","family":"Felix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0833-203X","authenticated-orcid":false,"given":"Val\u00e9ry","family":"Matarazzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3214-1724","authenticated-orcid":false,"given":"Muriel","family":"Thoby-Brisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4001-6528","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Muscatelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5069-482X","authenticated-orcid":false,"given":"Cl\u00e9ment","family":"Menuet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"2074_CR1","doi-asserted-by":"publisher","unstructured":"Menuet, C. et al. 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