{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:40:44Z","timestamp":1792489244181,"version":"4.5.0"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Neurosci"],"DOI":"10.1038\/s41593-026-02485-9","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:02:15Z","timestamp":1792486935000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Early lineage divergence segregates sensory and motor-associative identities in the thalamus"],"prefix":"10.1038","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-293X","authenticated-orcid":false,"given":"Lorenzo","family":"Puche-Aroca","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2061-9893","authenticated-orcid":false,"given":"Bel\u00e9n","family":"Andr\u00e9s-Bay\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2863-2339","authenticated-orcid":false,"given":"Emily S.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2686-8006","authenticated-orcid":false,"given":"Awais","family":"Javed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurelia","family":"Torregrosa-Mira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5470-1359","authenticated-orcid":false,"given":"Luis M.","family":"Rodr\u00edguez-Malmierca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6064-7584","authenticated-orcid":false,"given":"Jos\u00e9 P.","family":"L\u00f3pez-Atalaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8965-6836","authenticated-orcid":false,"given":"Guillermina","family":"L\u00f3pez-Bendito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"2485_CR1","doi-asserted-by":"publisher","first-page":"1068","DOI":"10.1152\/jn.00429.2011","volume":"106","author":"SM Sherman","year":"2011","unstructured":"Sherman, S. M. & Guillery, R. W. Distinct functions for direct and transthalamic corticocortical connections. J. Neurophysiol. 106, 1068\u20131077 (2011).","journal-title":"J. Neurophysiol."},{"key":"2485_CR2","doi-asserted-by":"publisher","first-page":"1669","DOI":"10.1038\/s41593-017-0020-1","volume":"20","author":"MM Halassa","year":"2017","unstructured":"Halassa, M. M. & Kastner, S. Thalamic functions in distributed cognitive control. Nat. Neurosci. 20, 1669\u20131679 (2017).","journal-title":"Nat. Neurosci."},{"key":"2485_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fncom.2013.00163","volume":"7","author":"C Bosch-Bouju","year":"2013","unstructured":"Bosch-Bouju, C., Hyland, B. I. & Parr-Brownlie, L. C. Motor thalamus integration of cortical, cerebellar and basal ganglia information: implications for normal and Parkinsonian conditions. Front. Comput. Neurosci. 7, 163 (2013).","journal-title":"Front. Comput. Neurosci."},{"key":"2485_CR4","first-page":"357","volume":"3","author":"F Clasc\u00e1","year":"2009","unstructured":"Clasc\u00e1, F., Rubio-Garrido, P., Galazo, M. J. & Porrero, C. Diversity in thalamic relay neurons: evidence for \u2018bottom-up\u2019 and \u2018top-down\u2019 information flow in thalamocortical pathways. An. R. Acad. Nac. Med. (Madr.) 3, 357\u2013372 (2009).","journal-title":"An. R. Acad. Nac. Med. (Madr.)"},{"key":"2485_CR5","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1038\/nn.2449","volume":"13","author":"BB Theyel","year":"2010","unstructured":"Theyel, B. B., Llano, D. A. & Sherman, S. M. The corticothalamocortical circuit drives higher-order cortex in the mouse. Nat. Neurosci. 13, 84\u201388 (2010).","journal-title":"Nat. Neurosci."},{"key":"2485_CR6","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.conb.2018.04.018","volume":"52","author":"N Ant\u00f3n-Bola\u00f1os","year":"2018","unstructured":"Ant\u00f3n-Bola\u00f1os, N., Espinosa, A. & L\u00f3pez-Bendito, G. Developmental interactions between thalamus and cortex: a true love reciprocal story. Curr. Opin. Neurobiol. 52, 33\u201341 (2018).","journal-title":"Curr. Opin. Neurobiol."},{"key":"2485_CR7","doi-asserted-by":"publisher","first-page":"eadr3675","DOI":"10.1126\/science.adr3675","volume":"388","author":"Z Fang","year":"2025","unstructured":"Fang, Z. et al. Human high-order thalamic nuclei gate conscious perception through the thalamofrontal loop. Science 388, eadr3675 (2025).","journal-title":"Science"},{"key":"2485_CR8","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1038\/nrn1075","volume":"4","author":"G L\u00f3pez-Bendito","year":"2003","unstructured":"L\u00f3pez-Bendito, G. & Moln\u00e1r, Z. Thalamocortical development: how are we going to get there? Nat. Rev. Neurosci. 4, 276\u2013289 (2003).","journal-title":"Nat. Rev. Neurosci."},{"key":"2485_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms16042","volume":"8","author":"D Jabaudon","year":"2017","unstructured":"Jabaudon, D. Fate and freedom in developing neocortical circuits. Nat. Commun. 8, 16042 (2017).","journal-title":"Nat. Commun."},{"key":"2485_CR10","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1038\/s41593-019-0483-3","volume":"22","author":"JW Phillips","year":"2019","unstructured":"Phillips, J. W. et al. A repeated molecular architecture across thalamic pathways. Nat. Neurosci. 22, 1925\u20131935 (2019).","journal-title":"Nat. Neurosci."},{"key":"2485_CR11","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1002\/cne.21467","volume":"505","author":"TY Vue","year":"2007","unstructured":"Vue, T. Y. et al. Characterization of progenitor domains in the developing mouse thalamus. J. Comp. Neurol. 505, 73\u201391 (2007).","journal-title":"J. Comp. Neurol."},{"key":"2485_CR12","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1002\/dneu.22460","volume":"77","author":"H Gezelius","year":"2017","unstructured":"Gezelius, H. & L\u00f3pez-Bendito, G. Thalamic neuronal specification and early circuit formation. Dev. Neurobiol. 77, 830\u2013843 (2017).","journal-title":"Dev. Neurobiol."},{"key":"2485_CR13","doi-asserted-by":"publisher","first-page":"3567","DOI":"10.1016\/j.cell.2025.04.003","volume":"188","author":"N Baumann","year":"2025","unstructured":"Baumann, N. et al. Regional differences in progenitor metabolism shape brain growth during development. Cell 188, 3567\u20133582 (2025).","journal-title":"Cell"},{"key":"2485_CR14","doi-asserted-by":"publisher","first-page":"dev202764","DOI":"10.1242\/dev.202764","volume":"151","author":"Q Lo Giudice","year":"2024","unstructured":"Lo Giudice, Q., Wagener, R. J., Abe, P., Frangeul, L. & Jabaudon, D. Developmental emergence of first- and higher-order thalamic neuron molecular identities. Development 151, dev202764 (2024).","journal-title":"Development"},{"key":"2485_CR15","doi-asserted-by":"publisher","first-page":"eadf9941","DOI":"10.1126\/science.adf9941","volume":"382","author":"CN Kim","year":"2023","unstructured":"Kim, C. N., Shin, D., Wang, A. & Nowakowski, T. J. Spatiotemporal molecular dynamics of the developing human thalamus. Science 382, eadf9941 (2023).","journal-title":"Science"},{"key":"2485_CR16","doi-asserted-by":"publisher","first-page":"dev174284","DOI":"10.1242\/dev.174284","volume":"146","author":"Q Guo","year":"2019","unstructured":"Guo, Q. & Li, J. Y. H. Defining developmental diversification of diencephalon neurons through single cell gene expression profiling. Development 146, dev174284 (2019).","journal-title":"Development"},{"key":"2485_CR17","doi-asserted-by":"publisher","first-page":"111768","DOI":"10.1016\/j.celrep.2022.111768","volume":"41","author":"KW Govek","year":"2022","unstructured":"Govek, K. W. et al. Developmental trajectories of thalamic progenitors revealed by single-cell transcriptome profiling and Shh perturbation. Cell Rep. 41, 111768 (2022).","journal-title":"Cell Rep."},{"key":"2485_CR18","doi-asserted-by":"publisher","first-page":"e2005211","DOI":"10.1371\/journal.pbio.2005211","volume":"16","author":"SZH Wong","year":"2018","unstructured":"Wong, S. Z. H. et al. In vivo clonal analysis reveals spatiotemporal regulation of thalamic nucleogenesis. PLoS Biol. 16, e2005211 (2018).","journal-title":"PLoS Biol."},{"key":"2485_CR19","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1038\/nn.4519","volume":"20","author":"W Shi","year":"2017","unstructured":"Shi, W. et al. Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus. Nat. Neurosci. 20, 516\u2013528 (2017).","journal-title":"Nat. Neurosci."},{"key":"2485_CR20","doi-asserted-by":"publisher","first-page":"2297","DOI":"10.1038\/s41596-018-0038-1","volume":"13","author":"S Govindan","year":"2018","unstructured":"Govindan, S., Oberst, P. & Jabaudon, D. In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag. Nat. Protoc. 13, 2297\u20132311 (2018).","journal-title":"Nat. Protoc."},{"key":"2485_CR21","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1126\/science.aad8361","volume":"351","author":"L Telley","year":"2016","unstructured":"Telley, L. et al. Sequential transcriptional waves direct the differentiation of newborn neurons in the mouse neocortex. Science 351, 1443\u20131446 (2016).","journal-title":"Science"},{"key":"2485_CR22","doi-asserted-by":"publisher","first-page":"eaav2522","DOI":"10.1126\/science.aav2522","volume":"364","author":"L Telley","year":"2019","unstructured":"Telley, L. et al. Temporal patterning of apical progenitors and their daughter neurons in the developing neocortex. Science 364, eaav2522 (2019).","journal-title":"Science"},{"key":"2485_CR23","doi-asserted-by":"publisher","first-page":"2711","DOI":"10.1523\/JNEUROSCI.21-08-02711.2001","volume":"21","author":"Y Nakagawa","year":"2001","unstructured":"Nakagawa, Y. & O\u2019Leary, D. D. M. Combinatorial expression patterns of LIM-homeodomain and other regulatory genes parcellate developing thalamus. J. Neurosci. 21, 2711\u20132725 (2001).","journal-title":"J. Neurosci."},{"key":"2485_CR24","doi-asserted-by":"publisher","first-page":"dev190181","DOI":"10.1242\/dev.190181","volume":"147","author":"MA Lipiec","year":"2020","unstructured":"Lipiec, M. A. et al. TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus. Development 147, dev190181 (2020).","journal-title":"Development"},{"key":"2485_CR25","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/S0925-4773(97)00110-X","volume":"67","author":"K Kitamura","year":"1997","unstructured":"Kitamura, K., Miura, H., Yanazawa, M., Miyashita, T. & Kato, K. Expression patterns of Brx1 (Rieg gene), Sonic hedgehog, Nkx2.2, Dlx1 and Arx during zona limitans intrathalamica and embryonic ventral lateral geniculate nuclear formation. Mech. Dev. 67, 83\u201396 (1997).","journal-title":"Mech. Dev."},{"key":"2485_CR26","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-024-05129-y","volume":"81","author":"W Choi","year":"2024","unstructured":"Choi, W. et al. RFX4 is an intrinsic factor for neuronal differentiation through induction of proneural genes POU3F2 and NEUROD1. Cell. Mol. Life Sci. 81, 99 (2024).","journal-title":"Cell. Mol. Life Sci."},{"key":"2485_CR27","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00031","volume":"14","author":"B Samata","year":"2020","unstructured":"Samata, B. et al. L1CAM is a marker for enriching corticospinal motor neurons in the developing brain. Front. Cell. Neurosci. 14, 31 (2020).","journal-title":"Front. Cell. Neurosci."},{"key":"2485_CR28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004559","volume":"11","author":"O Bar-Shira","year":"2015","unstructured":"Bar-Shira, O., Maor, R. & Chechik, G. Gene expression switching of receptor subunits in human brain development. PLoS Comput. Biol. 11, e1004559 (2015).","journal-title":"PLoS Comput. Biol."},{"key":"2485_CR29","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1038\/s41586-019-0969-x","volume":"566","author":"J Cao","year":"2019","unstructured":"Cao, J. et al. The single-cell transcriptional landscape of mammalian organogenesis. Nature 566, 496\u2013502 (2019).","journal-title":"Nature"},{"key":"2485_CR30","doi-asserted-by":"publisher","first-page":"4372","DOI":"10.1523\/JNEUROSCI.5851-11.2012","volume":"32","author":"P Marcos-Mond\u00e9jar","year":"2012","unstructured":"Marcos-Mond\u00e9jar, P. et al. The Lhx2 transcription factor controls thalamocortical axonal guidance by specific regulation of Robo1 and Robo2 receptors. J. Neurosci. 32, 4372\u20134385 (2012).","journal-title":"J. Neurosci."},{"key":"2485_CR31","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe8978","volume":"7","author":"\u00c1 Herrero-Navarro","year":"2021","unstructured":"Herrero-Navarro, \u00c1. et al. Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming. Sci. Adv. 7, eabe8978 (2021).","journal-title":"Sci. Adv."},{"key":"2485_CR32","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/nature19770","volume":"538","author":"L Frangeul","year":"2016","unstructured":"Frangeul, L. et al. A cross-modal genetic framework for the development and plasticity of sensory pathways. Nature 538, 96\u201398 (2016).","journal-title":"Nature"},{"key":"2485_CR33","doi-asserted-by":"publisher","first-page":"5621","DOI":"10.1242\/dev.00166","volume":"129","author":"S Garel","year":"2002","unstructured":"Garel, S., Yun, K., Grosschedl, R. & Rubenstein, J. L. R. The early topography of thalamocortical projections is shifted in Ebf1 and Dlx1\/2 mutant mice. Development 129, 5621\u20135634 (2002).","journal-title":"Development"},{"key":"2485_CR34","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/j.brainres.2008.07.096","volume":"1233","author":"WL Chen","year":"2008","unstructured":"Chen, W. L. et al. SCA8 mRNA expression suggests an antisense regulation of KLHL1 and correlates to SCA8 pathology. Brain Res. 1233, 176\u2013184 (2008).","journal-title":"Brain Res."},{"key":"2485_CR35","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1085\/jgp.201912457","volume":"151","author":"CS Bailey","year":"2019","unstructured":"Bailey, C. S., Moldenhauer, H. J., Park, S. M., Keros, S. & Meredith, A. L. KCNMA1-linked channelopathy. J. Gen. Physiol. 151, 1173\u20131189 (2019).","journal-title":"J. Gen. Physiol."},{"key":"2485_CR36","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1038\/s41586-022-05279-8","volume":"621","author":"JS Fleck","year":"2023","unstructured":"Fleck, J. S. et al. Inferring and perturbing cell fate regulomes in human brain organoids. Nature 621, 365\u2013372 (2023).","journal-title":"Nature"},{"key":"2485_CR37","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1038\/s41586-022-05688-9","volume":"614","author":"K Kamimoto","year":"2023","unstructured":"Kamimoto, K. et al. Dissecting cell identity via network inference and in silico gene perturbation. Nature 614, 742\u2013751 (2023).","journal-title":"Nature"},{"key":"2485_CR38","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1038\/s41586-021-04237-0","volume":"601","author":"RC Bandler","year":"2022","unstructured":"Bandler, R. C. et al. Single-cell delineation of lineage and genetic identity in the mouse brain. Nature 601, 404\u2013409 (2022).","journal-title":"Nature"},{"key":"2485_CR39","first-page":"5054","volume":"27","author":"H Gezelius","year":"2017","unstructured":"Gezelius, H. et al. Genetic labeling of nuclei-specific thalamocortical neurons reveals putative sensory-modality specific genes. Cereb. Cortex 27, 5054\u20135069 (2017).","journal-title":"Cereb. Cortex"},{"key":"2485_CR40","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14172","volume":"8","author":"V Moreno-Juan","year":"2017","unstructured":"Moreno-Juan, V. et al. Prenatal thalamic waves regulate cortical area size prior to sensory processing. Nat. Commun. 8, 14172 (2017).","journal-title":"Nat. Commun."},{"key":"2485_CR41","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1146\/annurev-neuro-111020-104222","volume":"45","author":"G L\u00f3pez-Bendito","year":"2022","unstructured":"L\u00f3pez-Bendito, G., An\u00edbal-Mart\u00ednez, M. & Martini, F. J. Cross-modal plasticity in brains deprived of visual input before vision. Annu. Rev. Neurosci. 45, 471\u2013489 (2022).","journal-title":"Annu. Rev. Neurosci."},{"key":"2485_CR42","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1038\/s41583-025-00945-y","volume":"26","author":"T Guillam\u00f3n-Vivancos","year":"2025","unstructured":"Guillam\u00f3n-Vivancos, T., An\u00edbal-Mart\u00ednez, M., Puche-Aroca, L., Martini, F. J. & L\u00f3pez-Bendito, G. Sensory modality-specific wiring of thalamocortical circuits. Nat. Rev. Neurosci. 26, 623\u2013641 (2025).","journal-title":"Nat. Rev. Neurosci."},{"key":"2485_CR43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-57052-w","volume":"16","author":"M An\u00edbal-Mart\u00ednez","year":"2025","unstructured":"An\u00edbal-Mart\u00ednez, M. et al. A prenatal window for enhancing spatial resolution of cortical barrel maps. Nat. Commun. 16, 1955 (2025).","journal-title":"Nat. Commun."},{"key":"2485_CR44","doi-asserted-by":"publisher","first-page":"2653","DOI":"10.1093\/cercor\/bhy133","volume":"29","author":"Z Liu","year":"2019","unstructured":"Liu, Z. et al. Sp9 regulates medial ganglionic eminence-derived cortical interneuron development. Cereb. Cortex 29, 2653\u20132667 (2019).","journal-title":"Cereb. Cortex"},{"key":"2485_CR45","doi-asserted-by":"publisher","DOI":"10.1242\/dev.165456","volume":"145","author":"Z Xu","year":"2018","unstructured":"Xu, Z. et al. SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression. Development 145, dev165456 (2018).","journal-title":"Development"},{"key":"2485_CR46","volume":"530","author":"SL Ding","year":"2021","unstructured":"Ding, S. L. et al. Cellular resolution anatomical and molecular atlases for prenatal human brains. J. Comp. Neurol. 530, 6-503 (2021).","journal-title":"J. Comp. Neurol."},{"key":"2485_CR47","doi-asserted-by":"publisher","first-page":"ENEURO.0367","DOI":"10.1523\/ENEURO.0367-18.2018","volume":"5","author":"Z Li","year":"2018","unstructured":"Li, Z., Pratt, T. & Price, D. J. Zic4-lineage cells increase their contribution to visual thalamic nuclei during murine embryogenesis if they are homozygous or heterozygous for loss of Pax6 function. eNeuro 5, ENEURO.0367 (2018).","journal-title":"eNeuro"},{"key":"2485_CR48","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","volume":"184","author":"Y Hao","year":"2021","unstructured":"Hao, Y. et al. Integrated analysis of multimodal single-cell data. Cell 184, 3573\u20133587 (2021).","journal-title":"Cell"},{"key":"2485_CR49","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.1016\/j.cell.2020.09.056","volume":"183","author":"S Ma","year":"2020","unstructured":"Ma, S. et al. Chromatin potential identified by shared single-cell profiling of RNA and chromatin. Cell 183, 1103\u20131116 (2020).","journal-title":"Cell"},{"key":"2485_CR50","doi-asserted-by":"publisher","first-page":"1531","DOI":"10.1073\/pnas.1008322108","volume":"108","author":"K Yusa","year":"2011","unstructured":"Yusa, K., Zhou, L., Li, M. A., Bradley, A. & Craig, N. L. A hyperactive piggyBac transposase for mammalian applications. Proc. Natl Acad. Sci. USA 108, 1531\u20131536 (2011).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2485_CR51","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1126\/science.aar4362","volume":"360","author":"DE Wagner","year":"2018","unstructured":"Wagner, D. E. et al. Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. Science 360, 981\u2013987 (2018).","journal-title":"Science"},{"key":"2485_CR52","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1038\/nbt.3437","volume":"34","author":"JG Doench","year":"2016","unstructured":"Doench, J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR\u2013Cas9. Nat. Biotechnol. 34, 184\u2013191 (2016).","journal-title":"Nat. Biotechnol."},{"key":"2485_CR53","doi-asserted-by":"publisher","first-page":"1479","DOI":"10.1016\/j.cell.2013.12.001","volume":"155","author":"B Chen","year":"2013","unstructured":"Chen, B. et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR\/Cas system. Cell 155, 1479\u20131491 (2013).","journal-title":"Cell"}],"container-title":["Nature Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41593-026-02485-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41593-026-02485-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41593-026-02485-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T09:02:16Z","timestamp":1792486936000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41593-026-02485-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,20]]},"references-count":53,"alternative-id":["2485"],"URL":"https:\/\/doi.org\/10.1038\/s41593-026-02485-9","relation":{},"ISSN":["1097-6256","1546-1726"],"issn-type":[{"value":"1097-6256","type":"print"},{"value":"1546-1726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,20]]},"assertion":[{"value":"17 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Ethics"}}]}}