{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:27:44Z","timestamp":1793237264328,"version":"4.5.0"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Struct Mol Biol"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1038\/s41594-021-00687-2","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T12:02:55Z","timestamp":1639051375000},"page":"982-988","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["The cryo-EM structure of the human neurofibromin dimer reveals the molecular basis for neurofibromatosis type 1"],"prefix":"10.1038","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4617-4853","authenticated-orcid":false,"given":"Christopher J.","family":"Lupton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7573-7715","authenticated-orcid":false,"given":"Charles","family":"Bayly-Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5765-8717","authenticated-orcid":false,"given":"Laura","family":"D\u2019Andrea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4575-1233","authenticated-orcid":false,"given":"Cheng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8738-1878","authenticated-orcid":false,"given":"Ralf B.","family":"Schittenhelm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari","family":"Venugopal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Whisstock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8555-8959","authenticated-orcid":false,"given":"Michelle L.","family":"Halls","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2248-9914","authenticated-orcid":false,"given":"Andrew M.","family":"Ellisdon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,9]]},"reference":[{"key":"687_CR1","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/s41568-018-0076-6","volume":"18","author":"S Li","year":"2018","unstructured":"Li, S., Balmain, A. & Counter, C. M. A model for RAS mutation patterns in cancers: finding the sweet spot. Nat. Rev. Cancer 18, 767\u2013777 (2018).","journal-title":"Nat. Rev. Cancer"},{"key":"687_CR2","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/nature04869","volume":"441","author":"RJ Shaw","year":"2006","unstructured":"Shaw, R. J. & Cantley, L. C. Ras, PI(3)K and mTOR signalling controls tumour cell growth. Nature 441, 424\u2013430 (2006).","journal-title":"Nature"},{"key":"687_CR3","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1016\/S0092-8674(01)00245-8","volume":"104","author":"K Cichowski","year":"2001","unstructured":"Cichowski, K. & Jacks, T. NF1 tumor suppressor gene function: narrowing the GAP. Cell 104, 593\u2013604 (2001).","journal-title":"Cell"},{"key":"687_CR4","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nrc3911","volume":"15","author":"N Ratner","year":"2015","unstructured":"Ratner, N. & Miller, S. J. A RASopathy gene commonly mutated in cancer: the neurofibromatosis type 1 tumour suppressor. Nat. Rev. Cancer 15, 290\u2013301 (2015).","journal-title":"Nat. Rev. Cancer"},{"key":"687_CR5","doi-asserted-by":"publisher","first-page":"17004","DOI":"10.1038\/nrdp.2017.4","volume":"3","author":"DH Gutmann","year":"2017","unstructured":"Gutmann, D. H. et al. Neurofibromatosis type 1. Nat. Rev. Dis. Prim. 3, 17004 (2017).","journal-title":"Nat. Rev. Dis. Prim."},{"key":"687_CR6","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1038\/s41586-020-1969-6","volume":"578","author":"Consortium, ITP-CAoWG.","year":"2020","unstructured":"Consortium, ITP-CAoWG. Pan-cancer analysis of whole genomes. Nature 578, 82\u201393 (2020).","journal-title":"Nature"},{"key":"687_CR7","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/nature22071","volume":"545","author":"NK Hayward","year":"2017","unstructured":"Hayward, N. K. et al. Whole-genome landscapes of major melanoma subtypes. Nature 545, 175\u2013180 (2017).","journal-title":"Nature"},{"key":"687_CR8","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1038\/nature11412","volume":"490","author":"Cancer Genome Atlas Network.","year":"2012","unstructured":"Cancer Genome Atlas Network. Comprehensive molecular portraits of human breast tumours. Nature 490, 61\u201370 (2012).","journal-title":"Nature"},{"key":"687_CR9","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/nature11404","volume":"489","author":"Cancer Genome Atlas Research Network.","year":"2012","unstructured":"Cancer Genome Atlas Research Network. Comprehensive genomic characterization of squamous cell lung cancers. Nature 489, 519\u2013525 (2012).","journal-title":"Nature"},{"key":"687_CR10","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1016\/j.cell.2013.09.034","volume":"155","author":"CW Brennan","year":"2013","unstructured":"Brennan, C. W. et al. The somatic genomic landscape of glioblastoma. Cell 155, 462\u2013477 (2013).","journal-title":"Cell"},{"key":"687_CR11","doi-asserted-by":"publisher","first-page":"D941","DOI":"10.1093\/nar\/gky1015","volume":"47","author":"JG Tate","year":"2019","unstructured":"Tate, J. G. et al. COSMIC: the catalogue of somatic mutations in cancer. Nucleic Acids Res. 47, D941\u2013D947 (2019).","journal-title":"Nucleic Acids Res."},{"key":"687_CR12","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1038\/s10038-019-0695-0","volume":"65","author":"E Kang","year":"2020","unstructured":"Kang, E. et al. Phenotype categorization of neurofibromatosis type I and correlation to NF1 mutation types. J. Hum. Genet 65, 79\u201389 (2020).","journal-title":"J. Hum. Genet"},{"key":"687_CR13","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1038\/356713a0","volume":"356","author":"TN Basu","year":"1992","unstructured":"Basu, T. N. et al. Aberrant regulation of ras proteins in malignant tumour cells from type 1 neurofibromatosis patients. Nature 356, 713\u2013715 (1992).","journal-title":"Nature"},{"key":"687_CR14","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1016\/S1470-2045(09)70033-6","volume":"10","author":"H Brems","year":"2009","unstructured":"Brems, H., Beert, E., de Ravel, T. & Legius, E. Mechanisms in the pathogenesis of malignant tumours in neurofibromatosis type 1. Lancet Oncol. 10, 508\u2013515 (2009).","journal-title":"Lancet Oncol."},{"key":"687_CR15","doi-asserted-by":"publisher","first-page":"8573","DOI":"10.1073\/pnas.0503224102","volume":"102","author":"CM Johannessen","year":"2005","unstructured":"Johannessen, C. M. et al. The NF1 tumor suppressor critically regulates TSC2 and mTOR. Proc. Natl Acad. Sci. USA 102, 8573\u20138578 (2005).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"687_CR16","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.cell.2008.09.060","volume":"135","author":"Y Cui","year":"2008","unstructured":"Cui, Y. et al. Neurofibromin regulation of ERK signaling modulates GABA release and learning. Cell 135, 549\u2013560 (2008).","journal-title":"Cell"},{"key":"687_CR17","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1016\/j.cell.2010.06.004","volume":"142","author":"M Holzel","year":"2010","unstructured":"Holzel, M. et al. NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome. Cell 142, 218\u2013229 (2010).","journal-title":"Cell"},{"key":"687_CR18","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1016\/j.celrep.2014.07.054","volume":"8","author":"MA Wolman","year":"2014","unstructured":"Wolman, M. A. et al. Modulation of cAMP and ras signaling pathways improves distinct behavioral deficits in a zebrafish model of neurofibromatosis type 1. Cell Rep. 8, 1265\u20131270 (2014).","journal-title":"Cell Rep."},{"key":"687_CR19","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1016\/S0021-9258(17)49919-4","volume":"295","author":"M Sherekar","year":"2020","unstructured":"Sherekar, M. et al. Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer. J. Biol. Chem. 295, 1105\u20131119 (2020).","journal-title":"J. Biol. Chem."},{"key":"687_CR20","doi-asserted-by":"publisher","first-page":"107909","DOI":"10.1016\/j.celrep.2020.107909","volume":"32","author":"W Yan","year":"2020","unstructured":"Yan, W. et al. Structural insights into the SPRED1-neurofibromin-KRAS complex and disruption of SPRED1-neurofibromin interaction by oncogenic EGFR. Cell Rep. 32, 107909 (2020).","journal-title":"Cell Rep."},{"key":"687_CR21","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1038\/sj.embor.7400602","volume":"7","author":"I D\u2019Angelo","year":"2006","unstructured":"D\u2019Angelo, I., Welti, S., Bonneau, F. & Scheffzek, K. A novel bipartite phospholipid-binding module in the neurofibromatosis type 1 protein. EMBO Rep. 7, 174\u2013179 (2006).","journal-title":"EMBO Rep."},{"key":"687_CR22","doi-asserted-by":"publisher","first-page":"22122","DOI":"10.1073\/pnas.1908353116","volume":"116","author":"D Rabara","year":"2019","unstructured":"Rabara, D. et al. KRAS G13D sensitivity to neurofibromin-mediated GTP hydrolysis. Proc. Natl Acad. Sci. USA 116, 22122\u201322131 (2019).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"687_CR23","doi-asserted-by":"publisher","first-page":"4313","DOI":"10.1093\/emboj\/17.15.4313","volume":"17","author":"K Scheffzek","year":"1998","unstructured":"Scheffzek, K. et al. Structural analysis of the GAP-related domain from neurofibromin and its implications. EMBO J. 17, 4313\u20134327 (1998).","journal-title":"EMBO J."},{"key":"687_CR24","doi-asserted-by":"publisher","first-page":"2992","DOI":"10.1016\/j.cub.2016.09.010","volume":"26","author":"K Xie","year":"2016","unstructured":"Xie, K. et al. NF1 is a direct G protein effector essential for opioid signaling to ras in the striatum. Curr. Biol. 26, 2992\u20133003 (2016).","journal-title":"Curr. Biol."},{"key":"687_CR25","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.ccell.2020.02.003","volume":"37","author":"ZY Zheng","year":"2020","unstructured":"Zheng, Z. Y. et al. Neurofibromin is an estrogen receptor-alpha transcriptional co-repressor in breast cancer. Cancer Cell 37, 387\u2013402 e7 (2020).","journal-title":"Cancer Cell"},{"key":"687_CR26","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1111\/jnc.13401","volume":"136","author":"X Koliou","year":"2016","unstructured":"Koliou, X., Fedonidis, C., Kalpachidou, T. & Mangoura, D. Nuclear import mechanism of neurofibromin for localization on the spindle and function in chromosome congression. J. Neurochem. 136, 78\u201391 (2016).","journal-title":"J. Neurochem."},{"key":"687_CR27","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/S0014-5793(03)01507-2","volume":"557","author":"L Feng","year":"2004","unstructured":"Feng, L. et al. PKA phosphorylation and 14-3-3 interaction regulate the function of neurofibromatosis type I tumor suppressor, neurofibromin. FEBS Lett. 557, 275\u2013282 (2004).","journal-title":"FEBS Lett."},{"key":"687_CR28","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1038\/sj.onc.1209113","volume":"25","author":"D Mangoura","year":"2006","unstructured":"Mangoura, D. et al. Phosphorylation of neurofibromin by PKC is a possible molecular switch in EGF receptor signaling in neural cells. Oncogene 25, 735\u2013745 (2006).","journal-title":"Oncogene"},{"key":"687_CR29","doi-asserted-by":"crossref","unstructured":"Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583\u2013589 (2021).","DOI":"10.1038\/s41586-021-03819-2"},{"key":"687_CR30","doi-asserted-by":"crossref","first-page":"3963","DOI":"10.1242\/jcs.185710","volume":"129","author":"SH Yoshimura","year":"2016","unstructured":"Yoshimura, S. H. & Hirano, T. HEAT repeats\u2014versatile arrays of amphiphilic helices working in crowded environments? J. Cell Sci. 129, 3963\u20133970 (2016).","journal-title":"J. Cell Sci."},{"key":"687_CR31","doi-asserted-by":"publisher","first-page":"7497","DOI":"10.1073\/pnas.1607298113","volume":"113","author":"T Dunzendorfer-Matt","year":"2016","unstructured":"Dunzendorfer-Matt, T., Mercado, E. L., Maly, K., McCormick, F. & Scheffzek, K. The neurofibromin recruitment factor Spred1 binds to the GAP related domain without affecting Ras inactivation. Proc. Natl Acad. Sci. USA 113, 7497\u20137502 (2016).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"687_CR32","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.ejmg.2016.11.001","volume":"60","author":"F Cali","year":"2017","unstructured":"Cali, F. et al. Mutation spectrum of NF1 gene in Italian patients with neurofibromatosis type 1 using Ion Torrent PGM platform. Eur. J. Med Genet 60, 93\u201399 (2017).","journal-title":"Eur. J. Med Genet"},{"key":"687_CR33","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1002\/1098-1004(200009)16:3<274::AID-HUMU21>3.0.CO;2-F","volume":"16","author":"E Girodon-Boulandet","year":"2000","unstructured":"Girodon-Boulandet, E. et al. NF1 gene analysis focused on CpG-rich exons in a cohort of 93 patients with neurofibromatosis type 1. Hum. Mutat. 16, 274\u2013275 (2000).","journal-title":"Hum. Mutat."},{"key":"687_CR34","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1093\/brain\/aws368","volume":"136","author":"IR Violante","year":"2013","unstructured":"Violante, I. R. et al. GABA deficit in the visual cortex of patients with neurofibromatosis type 1: genotype\u2013phenotype correlations and functional impact. Brain 136, 918\u2013925 (2013).","journal-title":"Brain"},{"key":"687_CR35","doi-asserted-by":"crossref","unstructured":"Melloni, G. et al. Risk of optic pathway glioma in neurofibromatosis type 1: no evidence of genotype\u2013phenotype correlations in a large independent cohort. Cancers 11, 1838 (2019).","DOI":"10.3390\/cancers11121838"},{"key":"687_CR36","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1111\/cge.12187","volume":"85","author":"R van Minkelen","year":"2014","unstructured":"van Minkelen, R. et al. A clinical and genetic overview of 18 years neurofibromatosis type 1 molecular diagnostics in the Netherlands. Clin. Genet 85, 318\u2013327 (2014).","journal-title":"Clin. Genet"},{"key":"687_CR37","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1158\/2159-8290.CD-12-0095","volume":"2","author":"E Cerami","year":"2012","unstructured":"Cerami, E. et al. The cBio cancer genomics portal: an open platform for exploring multidimensional cancer genomics data. Cancer Discov. 2, 401\u2013404 (2012).","journal-title":"Cancer Discov."},{"key":"687_CR38","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1038\/s41592-019-0580-y","volume":"16","author":"D Tegunov","year":"2019","unstructured":"Tegunov, D. & Cramer, P. Real-time cryo-electron microscopy data preprocessing with Warp. Nat. Methods 16, 1146\u20131152 (2019).","journal-title":"Nat. Methods"},{"key":"687_CR39","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nmeth.4169","volume":"14","author":"A Punjani","year":"2017","unstructured":"Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290\u2013296 (2017).","journal-title":"Nat. Methods"},{"key":"687_CR40","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/nmeth.4193","volume":"14","author":"SQ Zheng","year":"2017","unstructured":"Zheng, S. Q. et al. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat. Methods 14, 331\u2013332 (2017).","journal-title":"Nat. Methods"},{"key":"687_CR41","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","volume":"192","author":"A Rohou","year":"2015","unstructured":"Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216\u2013221 (2015).","journal-title":"J. Struct. Biol."},{"key":"687_CR42","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1038\/s42003-019-0437-z","volume":"2","author":"T Wagner","year":"2019","unstructured":"Wagner, T. et al. SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM. Commun. Biol. 2, 218 (2019).","journal-title":"Commun. Biol."},{"key":"687_CR43","doi-asserted-by":"publisher","first-page":"e42166","DOI":"10.7554\/eLife.42166","volume":"7","author":"J Zivanov","year":"2018","unstructured":"Zivanov, J. et al. New tools for automated high-resolution cryo-EM structure determination in RELION-3. eLife 7, e42166 (2018).","journal-title":"eLife"},{"key":"687_CR44","doi-asserted-by":"publisher","first-page":"107545","DOI":"10.1016\/j.jsb.2020.107545","volume":"211","author":"K Ramlaul","year":"2020","unstructured":"Ramlaul, K., Palmer, C. M., Nakane, T. & Aylett, C. H. S. Mitigating local over-fitting during single particle reconstruction with SIDESPLITTER. J. Struct. Biol. 211, 107545 (2020).","journal-title":"J. Struct. Biol."},{"key":"687_CR45","doi-asserted-by":"crossref","unstructured":"Grant, T., Rohou, A. & Grigorieff, N. cisTEM, user-friendly software for single-particle image processing. eLife 7, e35383 (2018).","DOI":"10.7554\/eLife.35383"},{"key":"687_CR46","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.jsb.2006.05.009","volume":"157","author":"G Tang","year":"2007","unstructured":"Tang, G. et al. EMAN2: an extensible image processing suite for electron microscopy. J. Struct. Biol. 157, 38\u201346 (2007).","journal-title":"J. Struct. Biol."},{"key":"687_CR47","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1107\/S0907444904019158","volume":"60","author":"P Emsley","year":"2004","unstructured":"Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D Biol. Crystallogr. 60, 2126\u20132132 (2004).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"687_CR48","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213\u2013221 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"687_CR49","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1107\/S2059798318002425","volume":"74","author":"TI Croll","year":"2018","unstructured":"Croll, T. I. ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps. Acta Crystallogr. D Struct. Biol. 74, 519\u2013530 (2018).","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"687_CR50","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1038\/nsmb.2235","volume":"19","author":"AM Ellisdon","year":"2012","unstructured":"Ellisdon, A. M., Dimitrova, L., Hurt, E. & Stewart, M. Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nat. Struct. Mol. Biol. 19, 328\u2013336 (2012).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"687_CR51","doi-asserted-by":"crossref","unstructured":"Cramer, P. AlphaFold2 and the future of structural biology. Nat. Struct. Mol. Biol. 28, 704\u2013705 (2021).","DOI":"10.1038\/s41594-021-00650-1"},{"key":"687_CR52","doi-asserted-by":"publisher","first-page":"904","DOI":"10.1038\/nmeth.2099","volume":"9","author":"B Yang","year":"2012","unstructured":"Yang, B. et al. Identification of cross-linked peptides from complex samples. Nat. Methods 9, 904\u2013906 (2012).","journal-title":"Nat. Methods"},{"key":"687_CR53","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1101\/gr.092759.109","volume":"19","author":"M Krzywinski","year":"2009","unstructured":"Krzywinski, M. et al. Circos: an information aesthetic for comparative genomics. Genome Res. 19, 1639\u20131645 (2009).","journal-title":"Genome Res."},{"key":"687_CR54","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1089\/adt.2015.643","volume":"13","author":"S Mondal","year":"2015","unstructured":"Mondal, S., Hsiao, K. & Goueli, S. A. A homogenous bioluminescent system for measuring GTPase, GTPase activating protein, and guanine nucleotide exchange factor activities. Assay Drug Dev. Technol. 13, 444\u2013455 (2015).","journal-title":"Assay Drug Dev. Technol."},{"key":"687_CR55","doi-asserted-by":"publisher","first-page":"1606","DOI":"10.1016\/S0006-3495(00)76713-0","volume":"78","author":"P Schuck","year":"2000","unstructured":"Schuck, P. Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling. Biophys. J. 78, 1606\u20131619 (2000).","journal-title":"Biophys. J."},{"key":"687_CR56","unstructured":"Laue, T. M., Shah, B., Ridgeway, T. M. & Pelletier, S. L. in Analytical Ultracentrifugation in Biochemistry and Polymer Science (eds Harding, S. E., et al.) pp. 90\u2013125 (Royal Society of Chemistry, 1992)."},{"key":"687_CR57","doi-asserted-by":"publisher","first-page":"D442","DOI":"10.1093\/nar\/gky1106","volume":"47","author":"Y Perez-Riverol","year":"2019","unstructured":"Perez-Riverol, Y. et al. The PRIDE database and related tools and resources in 2019: improving support for quantification data. Nucleic Acids Res. 47, D442\u2013D450 (2019).","journal-title":"Nucleic Acids Res."},{"key":"687_CR58","doi-asserted-by":"publisher","first-page":"D512","DOI":"10.1093\/nar\/gku1267","volume":"43","author":"PV Hornbeck","year":"2015","unstructured":"Hornbeck, P. V. et al. PhosphoSitePlus, 2014: mutations, PTMs and recalibrations. Nucleic Acids Res. 43, D512\u2013D520 (2015).","journal-title":"Nucleic Acids Res."},{"key":"687_CR59","doi-asserted-by":"publisher","first-page":"W344","DOI":"10.1093\/nar\/gkw408","volume":"44","author":"H Ashkenazy","year":"2016","unstructured":"Ashkenazy, H. et al. ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules. Nucleic Acids Res. 44, W344\u2013W350 (2016).","journal-title":"Nucleic Acids Res."}],"container-title":["Nature Structural &amp; Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41594-021-00687-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41594-021-00687-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41594-021-00687-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T23:42:07Z","timestamp":1726270927000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41594-021-00687-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":59,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["687"],"URL":"https:\/\/doi.org\/10.1038\/s41594-021-00687-2","relation":{},"ISSN":["1545-9993","1545-9985"],"issn-type":[{"value":"1545-9993","type":"print"},{"value":"1545-9985","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"20 April 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 December 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}