{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T19:58:20Z","timestamp":1772135900844,"version":"3.50.1"},"reference-count":61,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Struct Mol Biol"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1038\/s41594-025-01532-6","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T05:04:06Z","timestamp":1747890246000},"page":"1396-1407","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Structural elucidation of full-length Pfs48\/45 in complex with potent monoclonal antibodies isolated from a naturally exposed individual"],"prefix":"10.1038","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6150-3419","authenticated-orcid":false,"given":"Iga","family":"Kucharska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danton","family":"Ivanochko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Hailemariam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maartje R.","family":"Inklaar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee Ryung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"Teelen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rianne","family":"Stoter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marga","family":"van de Vegte-Bolmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert-Jan","family":"van Gemert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Semesi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon","family":"McLeod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1512-3660","authenticated-orcid":false,"given":"Ahyoung","family":"Ki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4040-1100","authenticated-orcid":false,"given":"Won-Kyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0566-2209","authenticated-orcid":false,"given":"John L.","family":"Rubinstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0686-370X","authenticated-orcid":false,"given":"Matthijs M.","family":"Jore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7602-3995","authenticated-orcid":false,"given":"Jean-Philippe","family":"Julien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"1532_CR1","unstructured":"WHO. World Malaria Report 2024 (WHO, 2024)."},{"key":"1532_CR2","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1086\/646611","volume":"200","author":"BF Hall","year":"2009","unstructured":"Hall, B. F. & Fauci, A. S. Malaria control, elimination, and eradication: the role of the evolving biomedical research agenda. J. Infect. Dis. 200, 1639\u20131643 (2009).","journal-title":"J. Infect. Dis."},{"key":"1532_CR3","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1080\/14760584.2021.1878028","volume":"20","author":"PE Duffy","year":"2021","unstructured":"Duffy, P. E. Transmission-blocking vaccines: harnessing herd immunity for malaria elimination. Expert Rev. Vaccines 20, 185\u2013198 (2021).","journal-title":"Expert Rev. Vaccines"},{"key":"1532_CR4","doi-asserted-by":"publisher","first-page":"102227","DOI":"10.1016\/j.mib.2022.102227","volume":"70","author":"PE Duffy","year":"2022","unstructured":"Duffy, P. E. Current approaches to malaria vaccines. Curr. Opin. Microbiol. 70, 102227 (2022).","journal-title":"Curr. Opin. Microbiol."},{"key":"1532_CR5","doi-asserted-by":"publisher","first-page":"2291","DOI":"10.1136\/bmj.p2291","volume":"383","author":"E Mahase","year":"2023","unstructured":"Mahase, E. WHO recommends second vaccine for malaria prevention in children. Brit. Med. J. 383, 2291 (2023).","journal-title":"Brit. Med. J."},{"key":"1532_CR6","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1080\/21645515.2019.1669415","volume":"16","author":"MB Laurens","year":"2020","unstructured":"Laurens, M. B. RTS,S\/AS01 vaccine (MosquirixTM): an overview. Hum. Vaccines Immunother. 16, 480\u2013489 (2020).","journal-title":"Hum. Vaccines Immunother."},{"key":"1532_CR7","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1056\/NEJMoa2203067","volume":"387","author":"RL Wu","year":"2022","unstructured":"Wu, R. L. et al. Low-dose subcutaneous or intravenous monoclonal antibody to prevent malaria. N. Engl. J. Med. 387, 397\u2013407 (2022).","journal-title":"N. Engl. J. Med."},{"key":"1532_CR8","doi-asserted-by":"publisher","first-page":"1833","DOI":"10.1056\/NEJMoa2206966","volume":"387","author":"K Kayentao","year":"2022","unstructured":"Kayentao, K. et al. Safety and efficacy of a monoclonal antibody against malaria in Mali. N. Engl. J. Med. 387, 1833\u20131842 (2022).","journal-title":"N. Engl. J. Med."},{"key":"1532_CR9","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1016\/j.medj.2023.07.003","volume":"4","author":"SE Silk","year":"2023","unstructured":"Silk, S. E. et al. Superior antibody immunogenicity of a viral-vectored RH5 blood-stage malaria vaccine in Tanzanian infants as compared to adults. Med 4, 668\u2013686 (2023).","journal-title":"Med"},{"key":"1532_CR10","doi-asserted-by":"publisher","first-page":"e2636","DOI":"10.1371\/journal.pone.0002636","volume":"3","author":"Y Wu","year":"2008","unstructured":"Wu, Y. et al. Phase 1 trial of malaria transmission blocking vaccine candidates Pfs25 and Pvs25 formulated with montanide ISA 51. PLoS ONE 3, e2636 (2008).","journal-title":"PLoS ONE"},{"key":"1532_CR11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI146221","volume":"131","author":"SA Healy","year":"2021","unstructured":"Healy, S. A. et al. Pfs230 yields higher malaria transmission-blocking vaccine activity than Pfs25 in humans but not mice. J. Clin. Invest. 131, e146221 (2021).","journal-title":"J. Clin. Invest."},{"key":"1532_CR12","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0092-8674(01)00199-4","volume":"104","author":"MR van Dijk","year":"2001","unstructured":"van Dijk, M. R. et al. A central role for P48\/45 in malaria parasite male gamete fertility. Cell 104, 153\u2013164 (2001).","journal-title":"Cell"},{"key":"1532_CR13","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/0166-6851(93)90158-T","volume":"61","author":"CHM Kocken","year":"1993","unstructured":"Kocken, C. H. M. et al. Cloning and expression of the gene coding for the transmission blocking target antigen Pfs48\/45 of Plasmodium falciparum. Mol. Biochem. Parasitol. 61, 59\u201368 (1993).","journal-title":"Mol. Biochem. Parasitol."},{"key":"1532_CR14","doi-asserted-by":"publisher","first-page":"4301","DOI":"10.1073\/pnas.0800459105","volume":"105","author":"NS Outchkourov","year":"2008","unstructured":"Outchkourov, N. S. et al. Correctly folded Pfs48\/45 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in mice. Proc. Natl Acad. Sci. USA 105, 4301\u20134305 (2008).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"1532_CR15","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1080\/14760584.2017.1276833","volume":"16","author":"M Theisen","year":"2017","unstructured":"Theisen, M., Jore, M. M. & Sauerwein, R. Towards clinical development of a Pfs48\/45-based transmission blocking malaria vaccine. Expert Rev. Vaccines 16, 329\u2013336 (2017).","journal-title":"Expert Rev. Vaccines"},{"key":"1532_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06340-9","volume":"9","author":"F Lennartz","year":"2018","unstructured":"Lennartz, F. et al. Structural basis for recognition of the malaria vaccine candidate Pfs48\/45 by a transmission blocking antibody. Nat. Commun. 9, 3822 (2018).","journal-title":"Nat. Commun."},{"key":"1532_CR17","doi-asserted-by":"publisher","first-page":"1680","DOI":"10.1016\/j.immuni.2022.07.015","volume":"55","author":"B McLeod","year":"2022","unstructured":"McLeod, B. et al. Vaccination with a structure-based stabilized version of malarial antigen Pfs48\/45 elicits ultra-potent transmission-blocking antibody responses. Immunity 55, 1680\u20131692.e8 (2022).","journal-title":"Immunity"},{"key":"1532_CR18","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-023-00619-9","volume":"8","author":"TH Dickey","year":"2023","unstructured":"Dickey, T. H. et al. Design of a stabilized non-glycosylated Pfs48\/45 antigen enables a potent malaria transmission-blocking nanoparticle vaccine. NPJ Vaccines 8, 20 (2023).","journal-title":"NPJ Vaccines"},{"key":"1532_CR19","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1016\/S1473-3099(22)00428-5","volume":"22","author":"SC van der Boor","year":"2022","unstructured":"van der Boor, S. C. et al. Safety, tolerability, and Plasmodium falciparum transmission-reducing activity of monoclonal antibody TB31F: a single-centre, open-label, first-in-human, dose-escalation, phase 1 trial in healthy malaria-naive adults. Lancet Infect. Dis. 22, 1596\u20131605 (2022).","journal-title":"Lancet Infect. Dis."},{"key":"1532_CR20","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06742-9","volume":"9","author":"P Kundu","year":"2018","unstructured":"Kundu, P. et al. Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48\/45. Nat. Commun. 9, 4458 (2018).","journal-title":"Nat. Commun."},{"key":"1532_CR21","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1006\/expr.2000.4586","volume":"97","author":"W Roeffen","year":"2001","unstructured":"Roeffen, W. et al. Plasmodium falciparum: production and characterization of rat monoclonal antibodies specific for the sexual-stage Pfs48\/45 antigen. Exp. Parasitol. 97, 45\u201349 (2001).","journal-title":"Exp. Parasitol."},{"key":"1532_CR22","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1016\/j.immuni.2023.01.009","volume":"56","author":"A Fabra-Garc\u00eda","year":"2023","unstructured":"Fabra-Garc\u00eda, A. et al. Highly potent, naturally acquired human monoclonal antibodies against Pfs48\/45 block Plasmodium falciparum transmission to mosquitoes. Immunity 56, 406\u2013419.e7 (2023).","journal-title":"Immunity"},{"key":"1532_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33379-6","volume":"13","author":"K-T Ko","year":"2022","unstructured":"Ko, K.-T. et al. Structure of the malaria vaccine candidate Pfs48\/45 and its recognition by transmission blocking antibodies. Nat. Commun. 13, 5603 (2022).","journal-title":"Nat. Commun."},{"key":"1532_CR24","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02646-2","volume":"9","author":"WJR Stone","year":"2018","unstructured":"Stone, W. J. R. et al. Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity. Nat. Commun. 9, 558 (2018).","journal-title":"Nat. Commun."},{"key":"1532_CR25","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nmeth.4169","volume":"14","author":"A Punjani","year":"2017","unstructured":"Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290\u2013296 (2017).","journal-title":"Nat. Methods"},{"key":"1532_CR26","doi-asserted-by":"publisher","first-page":"42","DOI":"10.12688\/wellcomeopenres.16168.1","volume":"6","author":"MalariaGEN","year":"2021","unstructured":"MalariaGEN et al. An open dataset of Plasmodium falciparum genome variation in 7,000 worldwide samples. Wellcome Open Res. 6, 42 (2021).","journal-title":"Wellcome Open Res."},{"key":"1532_CR27","doi-asserted-by":"publisher","first-page":"e0158212","DOI":"10.1371\/journal.pone.0158212","volume":"11","author":"Y Cao","year":"2016","unstructured":"Cao, Y., Bansal, G. P., Merino, K. & Kumar, N. Immunological cross-reactivity between malaria vaccine target antigen P48\/45 in Plasmodium vivax and P. falciparum and cross\u2013boosting of immune responses. PLoS ONE 11, e0158212 (2016).","journal-title":"PLoS ONE"},{"key":"1532_CR28","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1111\/j.1365-3024.1987.tb00511.x","volume":"9","author":"N Kumar","year":"1987","unstructured":"Kumar, N. Target antigens of malaria transmission blocking immunity exist as a stable membrane bound complex. Parasite Immunol. 9, 321\u2013335 (1987).","journal-title":"Parasite Immunol."},{"key":"1532_CR29","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1084\/jem.20031274","volume":"199","author":"G Pradel","year":"2004","unstructured":"Pradel, G. et al. A multidomain adhesion protein family expressed in Plasmodium falciparum is essential for transmission to the mosquito. J. Exp. Med. 199, 1533\u20131544 (2004).","journal-title":"J. Exp. Med."},{"key":"1532_CR30","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.parint.2015.09.007","volume":"65","author":"N Simon","year":"2016","unstructured":"Simon, N., Kuehn, A., Williamson, K. C. & Pradel, G. Adhesion protein complexes of malaria gametocytes assemble following parasite transmission to the mosquito. Parasitol. Int. 65, 27\u201330 (2016).","journal-title":"Parasitol. Int."},{"key":"1532_CR31","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28601-4","volume":"13","author":"RJ Ragotte","year":"2022","unstructured":"Ragotte, R. J. et al. Heterotypic interactions drive antibody synergy against a malaria vaccine candidate. Nat. Commun. 13, 933 (2022).","journal-title":"Nat. Commun."},{"key":"1532_CR32","doi-asserted-by":"publisher","first-page":"712538","DOI":"10.3389\/fmicb.2021.712538","volume":"12","author":"F Goerdeler","year":"2021","unstructured":"Goerdeler, F., Seeberger, P. H. & Moscovitz, O. Unveiling the sugary secrets of plasmodium parasites. Front. Microbiol. 12, 712538 (2021).","journal-title":"Front. Microbiol."},{"key":"1532_CR33","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1128\/EC.00197-09","volume":"9","author":"GG Bushkin","year":"2010","unstructured":"Bushkin, G. G. et al. Suggestive evidence for darwinian selection against asparagine-linked glycans of Plasmodium falciparum and Toxoplasma gondii. Eukaryot. Cell 9, 228\u2013241 (2010).","journal-title":"Eukaryot. Cell"},{"key":"1532_CR34","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1146\/annurev-biophys-060414-034156","volume":"47","author":"M Crispin","year":"2018","unstructured":"Crispin, M., Ward, A. B. & Wilson, I. A. Structure and immune recognition of the HIV glycan shield. Annu. Rev. Biophys. 47, 499\u2013523 (2018).","journal-title":"Annu. Rev. Biophys."},{"key":"1532_CR35","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25087-4","volume":"12","author":"A Antanasijevic","year":"2021","unstructured":"Antanasijevic, A. et al. Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM. Nat. Commun. 12, 4817 (2021).","journal-title":"Nat. Commun."},{"key":"1532_CR36","unstructured":"The PyMOL Molecular Graphics System (Schrodinger, 2015)."},{"key":"1532_CR37","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera\u2014a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605\u20131612 (2004).","journal-title":"J. Comput. Chem."},{"key":"1532_CR38","doi-asserted-by":"publisher","first-page":"1877","DOI":"10.1093\/bioinformatics\/btac016","volume":"38","author":"B Abanades","year":"2022","unstructured":"Abanades, B., Georges, G., Bujotzek, A. & Deane, C. M. ABlooper: fast accurate antibody CDR loop structure prediction with accuracy estimation. Bioinforma. Oxf. Engl. 38, 1877\u20131880 (2022).","journal-title":"Bioinforma. Oxf. Engl."},{"key":"1532_CR39","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1126\/science.abj8754","volume":"373","author":"M Baek","year":"2021","unstructured":"Baek, M. et al. Accurate prediction of protein structures and interactions using a three-track neural network. Science 373, 871\u2013876 (2021).","journal-title":"Science"},{"key":"1532_CR40","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1017\/S0031182000062065","volume":"98","author":"T Ponnudurai","year":"1989","unstructured":"Ponnudurai, T. et al. Infectivity of cultured Plasmodium falciparum gametocytes to mosquitoes. Parasitology 98, 165\u2013173 (1989).","journal-title":"Parasitology"},{"key":"1532_CR41","doi-asserted-by":"publisher","first-page":"e57909","DOI":"10.1371\/journal.pone.0057909","volume":"8","author":"K Miura","year":"2013","unstructured":"Miura, K. et al. Qualification of standard membrane-feeding assay with Plasmodium falciparum malaria and potential improvements for future assays. PLoS ONE 8, e57909 (2013).","journal-title":"PLoS ONE"},{"key":"1532_CR42","doi-asserted-by":"publisher","first-page":"2987","DOI":"10.1093\/bioinformatics\/btr509","volume":"27","author":"H Li","year":"2011","unstructured":"Li, H. A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing data. Bioinforma. Oxf. Engl. 27, 2987\u20132993 (2011).","journal-title":"Bioinforma. Oxf. Engl."},{"key":"1532_CR43","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.jsb.2013.11.002","volume":"185","author":"CR Marr","year":"2014","unstructured":"Marr, C. R., Benlekbir, S. & Rubinstein, J. L. Fabrication of carbon films with 500nm holes for cryo-EM with a direct detector device. J. Struct. Biol. 185, 42\u201347 (2014).","journal-title":"J. Struct. Biol."},{"key":"1532_CR44","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.jsb.2015.08.007","volume":"192","author":"JL Rubinstein","year":"2015","unstructured":"Rubinstein, J. L. & Brubaker, M. A. Alignment of cryo-EM movies of individual particles by optimization of image translations. J. Struct. Biol. 192, 188\u2013195 (2015).","journal-title":"J. Struct. Biol."},{"key":"1532_CR45","doi-asserted-by":"publisher","first-page":"1214","DOI":"10.1038\/s41592-020-00990-8","volume":"17","author":"A Punjani","year":"2020","unstructured":"Punjani, A., Zhang, H. & Fleet, D. J. Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction. Nat. Methods 17, 1214\u20131221 (2020).","journal-title":"Nat. Methods"},{"key":"1532_CR46","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39031-1","volume":"14","author":"J He","year":"2023","unstructured":"He, J., Li, T. & Huang, S.-Y. Improvement of cryo-EM maps by simultaneous local and non-local deep learning. Nat. Commun. 14, 3217 (2023).","journal-title":"Nat. Commun."},{"key":"1532_CR47","doi-asserted-by":"publisher","first-page":"107702","DOI":"10.1016\/j.jsb.2021.107702","volume":"213","author":"A Punjani","year":"2021","unstructured":"Punjani, A. & Fleet, D. J. 3D variability analysis: resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM. J. Struct. Biol. 213, 107702 (2021).","journal-title":"J. Struct. Biol."},{"key":"1532_CR48","doi-asserted-by":"publisher","first-page":"W474","DOI":"10.1093\/nar\/gkw361","volume":"44","author":"J Dunbar","year":"2016","unstructured":"Dunbar, J. et al. SAbPred: a structure-based antibody prediction server. Nucleic Acids Res. 44, W474\u2013W478 (2016).","journal-title":"Nucleic Acids Res."},{"key":"1532_CR49","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486\u2013501 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"1532_CR50","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1107\/S2059798318006551","volume":"74","author":"PV Afonine","year":"2018","unstructured":"Afonine, P. V. et al. Real-space refinement in PHENIX for cryo-EM and crystallography. Acta Crystallogr. D Struct. Biol. 74, 531\u2013544 (2018).","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"1532_CR51","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1107\/S0907444909042073","volume":"66","author":"VB Chen","year":"2010","unstructured":"Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr. D Biol. Crystallogr. 66, 12\u201321 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"1532_CR52","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1002\/pro.3235","volume":"27","author":"TD Goddard","year":"2018","unstructured":"Goddard, T. D. et al. UCSF ChimeraX: meeting modern challenges in visualization and analysis. Protein Sci. Publ. Protein Soc. 27, 14\u201325 (2018).","journal-title":"Protein Sci. Publ. Protein Soc."},{"key":"1532_CR53","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","volume":"372","author":"E Krissinel","year":"2007","unstructured":"Krissinel, E. & Henrick, K. Inference of macromolecular assemblies from crystalline state. J. Mol. Biol. 372, 774\u2013797 (2007).","journal-title":"J. Mol. Biol."},{"key":"1532_CR54","doi-asserted-by":"crossref","unstructured":"Kabsch, W. XDS. Acta Crystallogr. Acta Crystallogr. D Biol. Crystallogr. 66, 125\u2013132 (2010).","DOI":"10.1107\/S0907444909047337"},{"key":"1532_CR55","unstructured":"XPREP. X-ray data Preparation and Reciprocal space Exploration Program. (Bruker AXS, 2014)."},{"key":"1532_CR56","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"AJ McCoy","year":"2007","unstructured":"McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658\u2013674 (2007).","journal-title":"J. Appl. Crystallogr."},{"key":"1532_CR57","doi-asserted-by":"publisher","first-page":"1026","DOI":"10.1016\/j.cell.2015.04.028","volume":"161","author":"AG Schmidt","year":"2015","unstructured":"Schmidt, A. G. et al. Viral receptor-binding site antibodies with diverse germline origins. Cell 161, 1026\u20131034 (2015).","journal-title":"Cell"},{"key":"1532_CR58","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213\u2013221 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"1532_CR59","doi-asserted-by":"publisher","first-page":"e0145","DOI":"10.7554\/eLife.01456","volume":"2","author":"AB Morin","year":"2013","unstructured":"Morin, A. B. et al. Cutting edge: collaboration gets the most out of software. eLife 2, e0145 (2013).","journal-title":"eLife"},{"key":"1532_CR60","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","volume":"180","author":"SHW Scheres","year":"2012","unstructured":"Scheres, S. H. W. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519\u2013530 (2012).","journal-title":"J. Struct. Biol."},{"key":"1532_CR61","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1038\/s41592-019-0459-y","volume":"16","author":"GR Masson","year":"2019","unstructured":"Masson, G. R. et al. Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nat. Methods 16, 595\u2013602 (2019).","journal-title":"Nat. Methods"}],"container-title":["Nature Structural & Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41594-025-01532-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41594-025-01532-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41594-025-01532-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T15:47:17Z","timestamp":1757173637000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41594-025-01532-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,22]]},"references-count":61,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["1532"],"URL":"https:\/\/doi.org\/10.1038\/s41594-025-01532-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3605634\/v1","asserted-by":"object"}]},"ISSN":["1545-9993","1545-9985"],"issn-type":[{"value":"1545-9993","type":"print"},{"value":"1545-9985","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,22]]},"assertion":[{"value":"13 November 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}