{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T03:48:07Z","timestamp":1783828087888,"version":"3.55.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T00:00:00Z","timestamp":1579046400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T00:00:00Z","timestamp":1579046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001349","name":"MOH | National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/STaR\/0010\/2012"],"award-info":[{"award-number":["NMRC\/STaR\/0010\/2012"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001349","name":"MOH | National Medical Research Council","doi-asserted-by":"publisher","award":["MOH-000052"],"award-info":[{"award-number":["MOH-000052"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001349","name":"MOH | National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/BNIG\/2036\/2015"],"award-info":[{"award-number":["NMRC\/BNIG\/2036\/2015"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1038\/s41596-019-0270-3","type":"journal-article","created":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T17:05:09Z","timestamp":1579107909000},"page":"694-711","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Chemically defined and xenogeneic-free culture method for human epidermal keratinocytes on laminin-based matrices"],"prefix":"10.1038","volume":"15","author":[{"given":"Monica Suryana","family":"Tjin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvin Wen Choong","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Tryggvason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,15]]},"reference":[{"key":"270_CR1","volume":"9","author":"MS Tjin","year":"2018","unstructured":"Tjin, M. S. et al. Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture. Nat. Commun. 9, 4432 (2018).","journal-title":"Nat. Commun."},{"key":"270_CR2","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/0309-1651(80)90173-3","volume":"4","author":"BA Gilchrest","year":"1980","unstructured":"Gilchrest, B. A., Nemore, R. E. & Maciag, T. Growth of human keratinocytes on fibronectin-coated plates. Cell Biol. Int. Rep. 4, 1009\u20131016 (1980).","journal-title":"Cell Biol. Int. Rep."},{"key":"270_CR3","doi-asserted-by":"crossref","first-page":"e52494","DOI":"10.1371\/journal.pone.0052494","volume":"8","author":"R Lamb","year":"2013","unstructured":"Lamb, R. & Ambler, C. A. Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model. PLOS One 8, e52494 (2013).","journal-title":"PLOS One"},{"key":"270_CR4","first-page":"758","volume":"6","author":"Z-Z Xu","year":"2013","unstructured":"Xu, Z.-Z., Li, Z.-J., Du, L.-X., Li, J. & Wang, L.-Y. Using bovine pituitary extract to increase proliferation of keratinocytes and maintain their phenotype in vitro. Int. J. Opthalmol. 6, 758\u2013765 (2013).","journal-title":"Int. J. Opthalmol."},{"key":"270_CR5","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/j.1067-1927.2004.12609.x","volume":"12","author":"T Sun","year":"2004","unstructured":"Sun, T. et al. Developments in xenobiotic-free culture of human keratinocytes for clinical use. Wound Repair Regen. 12, 626\u2013634 (2004).","journal-title":"Wound Repair Regen."},{"key":"270_CR6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0092-8674(75)80001-8","volume":"6","author":"JG Rheinwald","year":"1975","unstructured":"Rheinwald, J. G. & Green, H. Serial cultivation of strains of human epidemal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6, 331\u2013343 (1975).","journal-title":"Cell"},{"key":"270_CR7","doi-asserted-by":"crossref","first-page":"4684","DOI":"10.3390\/ijms14034684","volume":"14","author":"F Bisson","year":"2013","unstructured":"Bisson, F. et al. Irradiated human dermal fibroblasts are as efficient as mouse fibroblasts as a feeder layer to improve human epidermal cell culture lifespan. Int. J. Mol. Sci. 14, 4684\u20134704 (2013).","journal-title":"Int. J. Mol. Sci."},{"issue":"1 Suppl","key":"270_CR8","doi-asserted-by":"crossref","first-page":"33s","DOI":"10.1111\/1523-1747.ep12540422","volume":"81","author":"S Boyce","year":"1983","unstructured":"Boyce, S. & Ham, R. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J. Invest. Dermatol. 81(1 Suppl), 33s\u201340s (1983).","journal-title":"J. Invest. Dermatol."},{"key":"270_CR9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1089\/ten.2006.12.245","volume":"12","author":"AJ Bullock","year":"2006","unstructured":"Bullock, A. J., Higham, M. C. & MacNeil, S. Use of human fibroblasts in the development of a xenobiotic-free culture and delivery system for human keratinocytes. Tissue Eng. 12, 245\u2013255 (2006).","journal-title":"Tissue Eng."},{"key":"270_CR10","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s10561-011-9247-3","volume":"13","author":"P De Corte","year":"2012","unstructured":"De Corte, P. et al. Feeder layer- and animal product-free culture of neonatal foreskin keratinocytes: improved performance, usability, quality and safety. Cell Tissue Bank 13, 175\u2013189 (2012).","journal-title":"Cell Tissue Bank"},{"key":"270_CR11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1159\/000225956","volume":"191","author":"L Panacchia","year":"2010","unstructured":"Panacchia, L. et al. Nonirradiated human fibroblasts and irradiated 3T3-J2 murine fibroblasts as a feeder layer for keratinocyte growth and differentiation in vitro on a fibrin substrate. Cells Tissues Organs 191, 21\u201335 (2010).","journal-title":"Cells Tissues Organs"},{"key":"270_CR12","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0140-6736(81)90006-4","volume":"1","author":"NE O\u2019Connor","year":"1981","unstructured":"O\u2019Connor, N. E., Mulliken, J. B., Banks-Schlegel, S., Kehinde, O. & Green, H. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1, 75\u201378 (1981).","journal-title":"Lancet"},{"key":"270_CR13","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1089\/107632703322495565","volume":"9","author":"MC Higham","year":"2003","unstructured":"Higham, M. C. et al. Development of a stable chemically defined surface for the culture of human keratinocytes under serum-free conditions for clinical use. Tissue Eng. 9, 919\u2013930 (2003).","journal-title":"Tissue Eng."},{"key":"270_CR14","doi-asserted-by":"crossref","first-page":"139","DOI":"10.15252\/emmm.201708213","volume":"10","author":"RE Hynds","year":"2018","unstructured":"Hynds, R. E., Bonfanti, P. & Janes, S. M. Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond. EMBO Mol. Med. 10, 139\u2013150 (2018).","journal-title":"EMBO Mol. Med."},{"key":"270_CR15","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1016\/j.burns.2015.08.011","volume":"41","author":"RM Chugh","year":"2015","unstructured":"Chugh, R. M., Chaturvedi, M. & Yerneni, L. K. An evaluation of the choice of feeder cell growth arrest for the production of cultured epidermis. Burns 41, 1788\u20131795 (2015).","journal-title":"Burns"},{"issue":"Suppl 1","key":"270_CR16","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1016\/0305-4179(92)90104-3","volume":"18","author":"ML Tenchini","year":"1992","unstructured":"Tenchini, M. L., Ranzati, C. & Malcovati, M. Culture techniques for human keratinocytes. Burns 18(Suppl 1), S11\u201315 (1992).","journal-title":"Burns"},{"key":"270_CR17","doi-asserted-by":"crossref","first-page":"12367","DOI":"10.1016\/S0021-9258(18)37764-0","volume":"263","author":"GA Weisman","year":"1988","unstructured":"Weisman, G. A. et al. Growth inhibition of transformed mouse fibroblasts by adenine nucleotides occurs via generation of extracellular adenosine. J. Biol. Chem. 263, 12367\u201312372 (1988).","journal-title":"J. Biol. Chem."},{"key":"270_CR18","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1002\/bies.20797","volume":"30","author":"H Green","year":"2008","unstructured":"Green, H. The birth of therapy with cultured cells. BioEssays 30, 897\u2013903 (2008).","journal-title":"BioEssays"},{"key":"270_CR19","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/cm.970020302","volume":"2","author":"JCR Jones","year":"1982","unstructured":"Jones, J. C. R., Goldman, A. E., Steinert, P. M., Yuspa, S. & Goldman, R. D. Dynamic aspects of the supramolecular organization of intermediate filament networks in cultural epidermal cells. Cell Motil. 2, 197\u2013213 (1982).","journal-title":"Cell Motil."},{"key":"270_CR20","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0305-4179(95)00092-5","volume":"22","author":"RA Dawson","year":"1996","unstructured":"Dawson, R. A., Goberdhan, N. J., Freedlander, E. & MacNeil, S. Influence of extracellular matrix proteins on human keratinocyte attachment, proliferation and transfer to a dermal wound model. Burns 22, 93\u2013100 (1996).","journal-title":"Burns"},{"key":"270_CR21","doi-asserted-by":"crossref","first-page":"649","DOI":"10.3727\/000000007783465046","volume":"16","author":"NA Coolen","year":"2007","unstructured":"Coolen, N. A. et al. Culture of keratinocytes for transplantation without the need of feeder layer cells. Cell Transplant. 16, 649\u2013661 (2007).","journal-title":"Cell Transplant."},{"key":"270_CR22","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1089\/ten.tea.2009.0251","volume":"16","author":"S Mujaj","year":"2010","unstructured":"Mujaj, S., Manton, K., Upton, Z. & Richards, S. Serum-free primary human fibroblast and keratinocyte coculture. Tissue Eng. Part A 16, 1407\u20131420 (2010).","journal-title":"Tissue Eng. Part A"},{"key":"270_CR23","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1002\/jbm.820221008","volume":"12","author":"ST Boyce","year":"1988","unstructured":"Boyce, S. T., Christianson, D. J. & Hansbrough, J. F. Structure of collagen-GAG dermal skin substitute optimized for cultured human epidermal keratinocytes. J. Biomed. Mater. Res. 12, 939\u2013957 (1988).","journal-title":"J. Biomed. Mater. Res."},{"key":"270_CR24","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/0022-202X(89)90196-6","volume":"92","author":"A Limat","year":"1989","unstructured":"Limat, A., Hunziker, T., Boillat, C., Bayreuther, K. & Noser, F. Post-mitotic human dermal fibroblasts efficiently support the growth of human follicular keratinocytes. J. Invest. Dermatol. 92, 758\u2013762 (1989).","journal-title":"J. Invest. Dermatol."},{"key":"270_CR25","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s10616-011-9382-5","volume":"63","author":"K Jubin","year":"2011","unstructured":"Jubin, K., Martin, Y., Lawrence-Watt, D. J. & Sharpe, J. R. A fully autologous co-culture system utilising non-irradiated autologous fibroblasts to support the expansion of human keratinocytes for clinical use. Cytotechnology 63, 655\u2013662 (2011).","journal-title":"Cytotechnology"},{"key":"270_CR26","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/1523-1747.ep12499961","volume":"98","author":"ER Waelti","year":"1992","unstructured":"Waelti, E. R. et al. Co-culture of human keratinocytes on post-mitotic human dermal fibroblast feeder cells: production of large amounts of interleukin 6. J. Invest. Dermatol. 98, 805\u2013808 (1992).","journal-title":"J. Invest. Dermatol."},{"key":"270_CR27","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.addr.2017.06.012","volume":"123","author":"B Ter Horst","year":"2018","unstructured":"Ter Horst, B., Chouhan, G., Moiemen, N. S. & Grover, L. M. Advances in keratinocyte delivery in burn wound care. Adv. Drug Deliv. Rev. 123, 18\u201332 (2018).","journal-title":"Adv. Drug Deliv. Rev."},{"key":"270_CR28","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/1523-1747.ep12475646","volume":"91","author":"Y Barrandon","year":"1988","unstructured":"Barrandon, Y., Li, V. & Green, H. New techniques for the grafting of cultured human epidermal cells onto athymic animals. J. Invest. Dermatol. 91, 315\u2013318 (1988).","journal-title":"J. Invest. Dermatol."},{"key":"270_CR29","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev.cellbio.20.010403.094555","volume":"20","author":"JH Miner","year":"2004","unstructured":"Miner, J. H. & Yurchenco, P. D. Laminin functions in tissue morphogenesis. Annu. Rev. Cell Dev. Biol. 20, 255\u2013284 (2004).","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"270_CR30","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1146\/annurev-cellbio-101011-155750","volume":"28","author":"A Domogatskaya","year":"2012","unstructured":"Domogatskaya, A., Rodin, S. & Tryggvason, K. Functional diversity of laminins. Annu. Rev. Cell Dev. Biol. 28, 523\u2013553 (2012).","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"270_CR31","doi-asserted-by":"crossref","first-page":"a004911","DOI":"10.1101\/cshperspect.a004911","volume":"3","author":"PD Yurchenco","year":"2011","unstructured":"Yurchenco, P. D. Basement membranes: cell scaffoldings and signaling platforms. Cold Spring Harb. Perspect. Biol. 3, a004911 (2011).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"270_CR32","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S0092-8674(02)00971-6","volume":"110","author":"RO Hynes","year":"2002","unstructured":"Hynes, R. O. Integrins: bidirectional, allosteric signaling machines. Cell 110, 673\u2013687 (2002).","journal-title":"Cell"},{"key":"270_CR33","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/ng0394-299","volume":"6","author":"D Aberdam","year":"1994","unstructured":"Aberdam, D. et al. Herlitz\u2019s junctional epidermolysis bullosa is linked to mutations in the gene (LAMC2) for the \u03b32 subunit of nicein\/kalinin (LAMININ-5). Nat. Genet. 6, 299\u2013304 (1994).","journal-title":"Nat. Genet."},{"key":"270_CR34","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/ng0394-293","volume":"6","author":"L Pulkkinen","year":"1994","unstructured":"Pulkkinen, L. et al. Mutations in the gamma 2 chain gene (LAMC2) of kalinin\/laminin 5 in the junctional forms of epidermolysis bullosa. Nat. Genet. 6, 293\u2013297 (1994).","journal-title":"Nat. Genet."},{"key":"270_CR35","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1034\/j.1600-0625.2002.110501.x","volume":"11","author":"N Pouliot","year":"2002","unstructured":"Pouliot, N., Saunders, N. A. & Kaur, P. Laminin 10\/11: an alternative adhesive ligand for epidermal keratinocytes with a functional role in promoting proliferation and migration. Exp. Dermatol. 11, 387\u2013397 (2002).","journal-title":"Exp. Dermatol."},{"key":"270_CR36","doi-asserted-by":"crossref","first-page":"14853","DOI":"10.1074\/jbc.275.20.14853","volume":"275","author":"J Kortesmaa","year":"2000","unstructured":"Kortesmaa, J., Yurchenco, P. & Tryggvason, K. Recombinant laminin-8 (\u03b14\u03b21\u03b31). Production, purification,and interactions with integrins. J. Biol. Chem. 275, 14853\u201314859 (2000).","journal-title":"J. Biol. Chem."},{"key":"270_CR37","doi-asserted-by":"crossref","first-page":"12741","DOI":"10.1074\/jbc.M111228200","volume":"277","author":"M Doi","year":"2002","unstructured":"Doi, M. et al. Recombinant human laminin-10 (\u03b15\u03b21\u03b31). Production, purification, and migration-promoting activity on vascular endothelial cells. J. Biol. Chem. 277, 12741\u201312748 (2002).","journal-title":"J. Biol. Chem."},{"key":"270_CR38","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms4195","volume":"5","author":"S Rodin","year":"2014","unstructured":"Rodin, S. et al. Clonal culturing of human embryonic stem cells on laminin-521\/E-cadherin matrix in defined and xeno-free environment. Nat. Commun. 5, 3195 (2014).","journal-title":"Nat. Commun."},{"key":"270_CR39","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1182\/blood.V97.1.312","volume":"97","author":"SF Parsons","year":"2001","unstructured":"Parsons, S. F. et al. Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind \u03b15 chain-containing human laminin with high affinity. Blood 97, 312 (2001).","journal-title":"Blood"},{"key":"270_CR40","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1038\/nprot.2014.159","volume":"9","author":"S Rodin","year":"2014","unstructured":"Rodin, S., Antonsson, L., Hovatta, O. & Tryggvason, K. Monolayer culturing and cloning of human pluripotent stem cells on laminin-521\u2013based matrices under xeno-free and chemically defined conditions. Nat. Protoc. 9, 2354\u20132368 (2014).","journal-title":"Nat. Protoc."},{"key":"270_CR41","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1038\/nbt.1620","volume":"28","author":"S Rodin","year":"2010","unstructured":"Rodin, S. et al. Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat. Biotech. 28, 611\u2013615 (2010).","journal-title":"Nat. Biotech."},{"key":"270_CR42","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1097\/00007890-199909270-00021","volume":"68","author":"G Pellegrini","year":"1999","unstructured":"Pellegrini, G. et al. The control of epidermal stem cells (holoclones) in the treatment of massive full-thickness burns with autologous keratinocytes cultured on fibrin. Transplantation 68, 868\u2013879 (1999).","journal-title":"Transplantation"},{"key":"270_CR43","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1177\/096368979800700309","volume":"7","author":"RE Horch","year":"1998","unstructured":"Horch, R. E., Bannasch, H., Kopp, J., Andree, C. & Stark, G. B. Single-cell suspensions of cultured human keratinocytes in fibrin-glue reconstitute the epidermis. Cell Transplant. 7, 309\u2013317 (1998).","journal-title":"Cell Transplant."},{"key":"270_CR44","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.burns.2007.07.009","volume":"34","author":"AWC Chua","year":"2008","unstructured":"Chua, A. W. C. et al. In vitro evaluation of fibrin mat and Tegaderm\u2122 wound dressing for the delivery of keratinocytes\u2014implications of their use to treat burns. Burns 34, 175\u2013180 (2008).","journal-title":"Burns"},{"key":"270_CR45","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1016\/j.burns.2018.01.021","volume":"44","author":"AWC Chua","year":"2018","unstructured":"Chua, A. W. C. et al. From skin allograft coverage to allograft\u2013micrograft sandwich method: a retrospective review of severe burn patients who received conjunctive application of cultured epithelial autografts. Burns 44, 1302\u20131307 (2018).","journal-title":"Burns"},{"key":"270_CR46","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1097\/00005373-198807000-00023","volume":"28","author":"AA Meyer","year":"1988","unstructured":"Meyer, A. A. et al. Antibody response to xenogeneic proteins in burned patients receiving cultured keratinocyte grafts. J. Trauma 28, 1054\u20131059 (1988).","journal-title":"J. Trauma"},{"key":"270_CR47","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nm1181","volume":"11","author":"MJ Martin","year":"2005","unstructured":"Martin, M. J., Muotri, A., Gage, F. & Varki, A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat. Med. 11, 228\u2013232 (2005).","journal-title":"Nat. Med."},{"key":"270_CR48","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1097\/00007890-200012150-00009","volume":"70","author":"V Ronfard","year":"2000","unstructured":"Ronfard, V., Rives, J. M., Neveux, Y., Carsin, H. & Barrandon, Y. Long-term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix. Transplantation 70, 1588\u20131598 (2000).","journal-title":"Transplantation"},{"key":"270_CR49","doi-asserted-by":"crossref","first-page":"553","DOI":"10.2217\/rme.13.43","volume":"8","author":"G Pellegrini","year":"2013","unstructured":"Pellegrini, G. et al. Biological parameters determining the clinical outcome of autologous cultures of limbal stem cells. Regen. Med. 8, 553\u2013567 (2013).","journal-title":"Regen. Med."},{"key":"270_CR50","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1172\/JCI200419140","volume":"113","author":"A Li","year":"2004","unstructured":"Li, A., Pouliot, N., Redvers, R. & Kaur, P. Extensive tissue-regenerative capacity of neonatal human keratinocyte stem cells and their progeny. J. Clin. Invest. 113, 390\u2013400 (2004).","journal-title":"J. Clin. Invest."}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/s41596-019-0270-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41596-019-0270-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41596-019-0270-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T22:29:32Z","timestamp":1684621772000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41596-019-0270-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,15]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["270"],"URL":"https:\/\/doi.org\/10.1038\/s41596-019-0270-3","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,15]]},"assertion":[{"value":"17 June 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 November 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 January 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"K.T. is a shareholder of BioLamina.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}