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In trypanosomatid parasites, conversely, gene regulation occurs mainly at the post-transcriptional level. However, our group has recently shown that hPTMs are abundant and varied in <jats:italic>Trypanosoma cruzi<\/jats:italic>, the etiological agent of Chagas Disease, signaling for possible conserved epigenetic functions. Here, we applied an optimized mass spectrometry-based proteomic workflow to provide a high-confidence comprehensive map of hPTMs, distributed in all canonical, variant and linker histones of <jats:italic>T. cruzi<\/jats:italic>. Our work expands the number of known <jats:italic>T. cruzi<\/jats:italic> hPTMs by almost 2-fold, representing the largest dataset of hPTMs available to any trypanosomatid to date, and can be used as a basis for functional studies on the dynamic regulation of chromatin by epigenetic mechanisms and the selection of candidates for the development of epigenetic drugs against trypanosomatids.<\/jats:p>","DOI":"10.1038\/s41597-021-00818-w","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T11:06:58Z","timestamp":1616670418000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["An updated map of Trypanosoma cruzi histone post-translational modifications"],"prefix":"10.1038","volume":"8","author":[{"given":"Rafael Foga\u00e7a","family":"de Almeida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4316-8412","authenticated-orcid":false,"given":"Matheus","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyris Martins Franco","family":"de Godoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,25]]},"reference":[{"key":"818_CR1","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1590\/S0074-02761909000200008","volume":"1","author":"C Chagas","year":"1909","unstructured":"Chagas, C. 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