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The mosquito midgut is the principal organ for blood meal digestion, nutrient absorption and the initial site of arbovirus infection. Although a previous study delineated midgut\u2019s transcriptome of <jats:italic>Ae. aegypti<\/jats:italic> at the single-nucleus resolution, there still lacks an established protocol for isolating and RNA sequencing of single cells of <jats:italic>Ae. aegypti<\/jats:italic> midgut, which is required for investigating arbovirus-midgut interaction at the single-cell level. Here, we established an atlas of the midgut cells for <jats:italic>Ae. aegypti<\/jats:italic> by single-cell RNA sequencing. We annotated the cell clusters including intestinal stem cells\/enteroblasts (ISC\/EB), cardia cells (Cardia), enterocytes (EC, EC-like), enteroendocrine cells (EE), visceral muscle (VM), fat body cells (FBC) and hemocyte cells (HC). This study will provide a foundation for further studies of arbovirus infection in mosquito midgut at the single-cell level.<\/jats:p>","DOI":"10.1038\/s41597-024-03432-8","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T12:17:13Z","timestamp":1717589833000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["A cell atlas of the adult female Aedes aegypti midgut revealed by single-cell RNA sequencing"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1310-9817","authenticated-orcid":false,"given":"Shunlong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6411-497X","authenticated-orcid":false,"given":"Ying","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6245-5252","authenticated-orcid":false,"given":"Qian","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanjie","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjun","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-6040","authenticated-orcid":false,"given":"Han","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,5]]},"reference":[{"key":"3432_CR1","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.meegid.2018.11.009","volume":"67","author":"JA Souza-Neto","year":"2019","unstructured":"Souza-Neto, J. 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