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Here, we present a chromosome-level reference genome of <jats:italic>T. foetus<\/jats:italic> -KV-1 (ATCC 30924) using short-read (Illumina Miseq), long-read (Oxford Nanopore) and chromatin-linked (Hi-C) sequencing. This is the first chromosome-level genome of a parasitic protist of the order Tritrichomonadida and the second within the Parabasalia lineage, after <jats:italic>Trichomonas vaginalis<\/jats:italic>, the human-associated causative agent of the sexually transmitted infection in humans. Our constructed genome is 148\u2009Mb in size, with a N50 length of the scaffolds of 22.9\u2009Mb. The contigs are anchored in five super-scaffolds, corresponding to the expected five chromosomes of the species and covering 78% of the genome assembly. We predict 41,341 protein-coding genes, of which 95.10% have been functionally annotated. This high-quality genome assembly serves as a valuable reference genome for <jats:italic>T. foetus<\/jats:italic> to support future studies in functional genomics, genetic conservation and taxonomy.<\/jats:p>","DOI":"10.1038\/s41597-024-03818-8","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T09:24:55Z","timestamp":1727169895000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Chromosome-level genome assembly of Tritrichomonas foetus, the causative agent of Bovine Trichomonosis"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2068-6081","authenticated-orcid":false,"given":"Mostafa Y.","family":"Abdel-Glil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Solle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Wibberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinrich","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa D.","family":"Sprague","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,20]]},"reference":[{"key":"3818_CR1","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1016\/j.theriogenology.2015.10.037","volume":"85","author":"AN Michi","year":"2016","unstructured":"Michi, A. 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