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In both spermatogenesis and oogenesis, post-transcriptional gene expression regulation is crucial. Essentially, all mRNAs possess non-templated poly(A) tails, whose composition and dynamics (elongation, shortening, and modifications) determine the fate of mRNA. Moreover, gametogenesis, especially oogenesis, represents a unique instance of the complexity of poly(A) tails metabolism, with oocyte-specific waves of cytoplasmic polyadenylation. In this context, we provide a comprehensive transcriptomic dataset focusing on mRNA poly(A) tail composition and dynamics in murine testes and ovaries. It consists of RNA samples isolated from wild-type and transgenic mice lacking TENT5 polymerases, which can extend poly(A) tails in the cytoplasm. TENT5 deficiencies have serious consequences. For instance, the defect of TENT5D causes infertility in humans. The data described here are generated mainly using the Oxford Nanopore Direct RNA Sequencing (DRS) method, which provides ground-truth information about mRNA molecules, including poly(A) tail length and nucleotide content. For instance, we show the prevalence of uridilated tails in testicular mRNAs.<\/jats:p>","DOI":"10.1038\/s41597-024-04226-8","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T09:29:11Z","timestamp":1736501351000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing"],"prefix":"10.1038","volume":"12","author":[{"given":"Agnieszka","family":"Czarnocka-Cieciura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Brouze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4599-2897","authenticated-orcid":false,"given":"Natalia","family":"Gumi\u0144ska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seweryn","family":"Mroczek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Gewartowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9531-1298","authenticated-orcid":false,"given":"Pawe\u0142 S.","family":"Krawczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8492-7572","authenticated-orcid":false,"given":"Andrzej","family":"Dziembowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,10]]},"reference":[{"key":"4226_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49479-4","volume":"15","author":"M Brouze","year":"2024","unstructured":"Brouze, M. et al. TENT5-mediated polyadenylation of mRNAs encoding secreted proteins is essential for gametogenesis in mice. Nat Commun 15, 5331, https:\/\/doi.org\/10.1038\/s41467-024-49479-4 (2024).","journal-title":"Nat Commun"},{"key":"4226_CR2","doi-asserted-by":"publisher","unstructured":"Zhang, Y. T. et al. Novel variations in TENT5D lead to teratozoospermia in infertile patients. Andrology-Us, https:\/\/doi.org\/10.1111\/andr.13589 (2024).","DOI":"10.1111\/andr.13589"},{"key":"4226_CR3","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.1111\/andr.13407","volume":"11","author":"Y Sha","year":"2023","unstructured":"Sha, Y. et al. TENT5D disruption causes oligoasthenoteratozoospermia and male infertility. 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