{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:48:15Z","timestamp":1789757295798,"version":"4.0.1"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T00:00:00Z","timestamp":1736899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T00:00:00Z","timestamp":1736899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Data"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Chinese hamster ovary (CHO) cells play a pivotal role in the production of recombinant therapeutics. In the present study, we conducted a genome-scale pooled CRISPR knockout (KO) screening using a virus-free, recombinase-mediated cassette exchange-based platform in CHO-K1 host and CHO-K1 derived recombinant cells. Genome-wide guide RNA (gRNA) amplicon sequencing data were generated from cell libraries, as well as short- and long-term KO libraries, and validated through phenotypic assessment and gRNA read count distribution. Additionally, we obtained gRNA amplicon sequencing data from the highly productive recombinant cell populations. By analyzing these datasets, essential genes involved in cell fitness as well as functional target genes associated with therapeutic protein production can be identified. Collectively, our next-generation sequencing datasets, derived from a robust and reliable CRISPR screening method, provide valuable insights into CHO genomic functions, advancing the development of next-generation CHO factories.<\/jats:p>","DOI":"10.1038\/s41597-025-04438-6","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T07:24:54Z","timestamp":1736925894000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive genome-scale CRISPR knockout screening of CHO cells"],"prefix":"10.1038","volume":"12","author":[{"given":"Sung Wook","family":"Shin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Hyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aghiles","family":"Gasselin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9876-7378","authenticated-orcid":false,"given":"Gyun Min","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3291-2093","authenticated-orcid":false,"given":"Jae Seong","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,15]]},"reference":[{"key":"4438_CR1","doi-asserted-by":"publisher","first-page":"1722","DOI":"10.1038\/s41587-022-01582-x","volume":"40","author":"G Walsh","year":"2022","unstructured":"Walsh, G. & Walsh, E. Biopharmaceutical benchmarks 2022. Nat Biotechnol 40, 1722\u20131760, https:\/\/doi.org\/10.1038\/s41587-022-01582-x (2022).","journal-title":"Nat Biotechnol"},{"key":"4438_CR2","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1016\/j.biotechadv.2011.08.022","volume":"30","author":"J Zhu","year":"2012","unstructured":"Zhu, J. Mammalian cell protein expression for biopharmaceutical production. Biotechnology advances 30, 1158\u20131170 (2012).","journal-title":"Biotechnology advances"},{"key":"4438_CR3","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/j.coche.2018.09.011","volume":"22","author":"NK Hamaker","year":"2018","unstructured":"Hamaker, N. K. & Lee, K. H. Site-specific Integration Ushers in a New Era of Precise CHO Cell Line Engineering. 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