{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T11:11:58Z","timestamp":1772017918768,"version":"3.50.1"},"reference-count":74,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Data"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>In this study, we infected Holstein calves with <jats:italic>Ostertagia ostertagi<\/jats:italic> stage 3 larvae (L3) and determined gene expression profiles of abomasal fundic and pyloric mucosa by RNA sequencing (RNA-seq) at 3\u20135, 7\u20139, 10, and 21 days post-infection (dpi), which represent late L3 and early L4 (between 3\u20135 dpi), mid to late L4 (between 7\u20139 and 10 dpi) and adult stages (21 dpi) of the parasitic stage. Bioinformatics analyses were performed to profile the transcriptomic changes over time as well as between cattle abomasal tissues. The results will help understand the gastric responses of the host, especially the immune responses, at different phases of early nematode infection. Such an undertaking is crucial for an in-depth comprehension of host responses, where immunologic reagents for cattle are highly limited. The RNA-seq datasets generated in this study provide a vital data resource, allowing for future comparative analyses to similar data resources or using more advanced analytical technologies in mechanisms of host-parasite interactions.<\/jats:p>","DOI":"10.1038\/s41597-025-04640-6","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T15:19:12Z","timestamp":1740151152000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Time-dependent RNA transcriptional profiling of abomasal mucosa in cattle infected with Ostertagia ostertagi"],"prefix":"10.1038","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6575-5266","authenticated-orcid":false,"given":"Clarissa","family":"Boschiero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ethiopia","family":"Beshah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariam","family":"Bakshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0192-6705","authenticated-orcid":false,"given":"George E.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-8981","authenticated-orcid":false,"given":"Wenbin","family":"Tuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"4640_CR1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.3389\/fvets.2018.00255","volume":"5","author":"F Vande Velde","year":"2018","unstructured":"Vande Velde F., Charlier J., Claerebout E. Farmer Behavior and Gastrointestinal Nematodes in Ruminant Livestock-Uptake of Sustainable Control Approaches. Front Vet Sci. 5, 255 (2018).","journal-title":"Front Vet Sci."},{"issue":"13\u201314","key":"4640_CR2","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1016\/j.ijpara.2021.10.007","volume":"51","author":"JS Gilleard","year":"2021","unstructured":"Gilleard, J. S. et al. A journey through 50 years of research relevant to the control of gastrointestinal nematodes in ruminant livestock and thoughts on future directions. Int J Parasitol. 51(13\u201314), 1133\u20131151 (2021).","journal-title":"Int J Parasitol."},{"issue":"3-4","key":"4640_CR3","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/S0304-4017(97)00102-7","volume":"72","author":"MT Fox","year":"1997","unstructured":"Fox, M. T. Pathophysiology of infection with gastrointestinal nematodes in domestic ruminants: recent developments. Vet. Parasitol. 72(3-4), 285\u2013308 (1997).","journal-title":"Vet. Parasitol."},{"key":"4640_CR4","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.vetpar.2016.10.005","volume":"229","author":"W Tuo","year":"2016","unstructured":"Tuo, W. et al. Abomasal mucosal immune responses of cattle with limited or continuous exposure to pasture-borne gastrointestinal nematode parasite infection. Vet. Parasitol. 229, 118\u2013125 (2016).","journal-title":"Vet. Parasitol."},{"issue":"5","key":"4640_CR5","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1101\/gr.250704.119","volume":"30","author":"L Fang","year":"2020","unstructured":"Fang, L. et al. Comprehensive analyses of 723 transcriptomes enhance genetic and biological interpretations for complex traits in cattle. Genome Res. 30(5), 790\u2013801 (2020).","journal-title":"Genome Res."},{"issue":"9","key":"4640_CR6","doi-asserted-by":"publisher","first-page":"1438","DOI":"10.1038\/s41588-022-01153-5","volume":"54","author":"S Liu","year":"2022","unstructured":"Liu, S. et al. A multi-tissue atlas of regulatory variants in cattle. Nature Genetics 54(9), 1438\u20131447 (2022).","journal-title":"Nature Genetics"},{"issue":"1","key":"4640_CR7","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1186\/1297-9716-42-114","volume":"42","author":"RW Li","year":"2011","unstructured":"Li, R. W., Rinaldi, M. & Capuco, A. V. Characterization of the abomasal transcriptome for mechanisms of resistance to gastrointestinal nematodes in cattle. Vet Res. 42(1), 114 (2011).","journal-title":"Vet Res."},{"issue":"9-10","key":"4640_CR8","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.ijpara.2015.03.007","volume":"45","author":"F Van Meulder","year":"2015","unstructured":"Van Meulder, F. et al. Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora. Int J Parasitol. 45(9-10), 637\u201346 (2015).","journal-title":"Int J Parasitol."},{"issue":"1","key":"4640_CR9","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1017\/S0022149X00004004","volume":"43","author":"JH Rose","year":"1969","unstructured":"Rose, J. H. The development of the parasitic stages of Ostertagia ostertagi. J Helminthol. 43(1), 173\u201384 (1969).","journal-title":"J Helminthol."},{"key":"4640_CR10","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/0304-4017(94)90060-4","volume":"55","author":"LC Gasbarre","year":"1994","unstructured":"Gasbarre, L. C. Ostertagia ostertagi: changes in lymphoid populations in the local lymphoid tissues after primary or secondary infection. Vet Parasitol. 55, 105\u201314 (1994).","journal-title":"Vet Parasitol."},{"key":"4640_CR11","doi-asserted-by":"crossref","unstructured":"Conesa, A. et al. A survey of best practices for RNA-seq data analysis. Genome Biol. 17, 13 (2016). Erratum in: Genome Biol. 17(1), 181 (2016).","DOI":"10.1186\/s13059-016-1047-4"},{"issue":"16","key":"4640_CR12","doi-asserted-by":"publisher","first-page":"2184","DOI":"10.1093\/bioinformatics\/bts356","volume":"28","author":"L Wang","year":"2012","unstructured":"Wang, L., Wang, S. & Li, W. RSeQC: quality control of RNA-seq experiments. Bioinformatics 28(16), 2184\u20135 (2012).","journal-title":"Bioinformatics"},{"issue":"15","key":"4640_CR13","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30(15), 2114\u201320 (2014).","journal-title":"Bioinformatics"},{"issue":"3","key":"4640_CR14","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giaa021","volume":"9","author":"BD Rosen","year":"2020","unstructured":"Rosen, B. D. et al. De novo assembly of the cattle reference genome with single-molecule sequencing. Gigascience 9(3), giaa021 (2020).","journal-title":"Gigascience"},{"issue":"8","key":"4640_CR15","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","volume":"37","author":"D Kim","year":"2019","unstructured":"Kim, D., Paggi, J. M., Park, C., Bennett, C. & Salzberg, S. L. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat. Biotechnol. 37(8), 907\u2013915 (2019).","journal-title":"Nat. Biotechnol."},{"issue":"16","key":"4640_CR16","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li, H. et al. The Sequence alignment\/map (SAM) format and SAMtools. Bioinformatics 25(16), 2078\u20139 (2009).","journal-title":"Bioinformatics"},{"issue":"2","key":"4640_CR17","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","volume":"31","author":"S Anders","year":"2015","unstructured":"Anders, S., Pyl, P. T. & Huber, W. HTSeq\u2013a Python framework to work with high-throughput sequencing data. Bioinformatics 31(2), 166\u20139 (2015).","journal-title":"Bioinformatics"},{"key":"4640_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15, 550 (2014).","journal-title":"Genome Biol"},{"key":"4640_CR19","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106","volume":"11","author":"S Anders","year":"2010","unstructured":"Anders, S. & Huber, W. Differential expression analysis for sequence count data. Genome Biol 11, R106 (2010).","journal-title":"Genome Biol"},{"key":"4640_CR20","doi-asserted-by":"crossref","unstructured":"Borg, I. & Groenen, P. Modern Multidimensional Scaling. Theory and Applications (Springer, 1997).","DOI":"10.1007\/978-1-4757-2711-1"},{"issue":"5","key":"4640_CR21","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1093\/bioinformatics\/bti042","volume":"21","author":"I Yanai","year":"2005","unstructured":"Yanai, I. et al. Genome-wide midrange transcription profiles reveal expression level relationships in human tissue specification. Bioinformatics 21(5), 650\u20139 (2005).","journal-title":"Bioinformatics"},{"key":"4640_CR22","doi-asserted-by":"crossref","unstructured":"Camargo, A. P., Vasconcelos, A. A., Fiamenghi, M. B., Pereira, G. A. G. & Carazzolle, M. F. Tspex: a tissue-specificity calculator for gene expression data. Preprint at https:\/\/www.researchsquare.com\/article\/rs-51998\/v1 (2020).","DOI":"10.21203\/rs.3.rs-51998\/v1"},{"issue":"8","key":"4640_CR23","doi-asserted-by":"publisher","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","volume":"36","author":"SX Ge","year":"2020","unstructured":"Ge, S. X., Jung, D. & Yao, R. ShinyGO: a graphical gene-set enrichment tool for animals and plants. Bioinformatics 36(8), 2628\u20132629 (2020).","journal-title":"Bioinformatics"},{"issue":"9","key":"4640_CR24","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1093\/bioinformatics\/btl056","volume":"22","author":"A Conesa","year":"2006","unstructured":"Conesa, A., Nueda, M. J., Ferrer, A. & Tal\u00f3n, M. maSigPro: a method to identify significantly differential expression profiles in time-course microarray experiments. Bioinformatics 22(9), 1096\u2013102 (2006).","journal-title":"Bioinformatics"},{"key":"4640_CR25","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247730 (2024)."},{"key":"4640_CR26","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247731 (2024)."},{"key":"4640_CR27","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247732 (2024)."},{"key":"4640_CR28","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247733 (2024)."},{"key":"4640_CR29","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247734 (2024)."},{"key":"4640_CR30","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247735 (2024)."},{"key":"4640_CR31","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247736 (2024)."},{"key":"4640_CR32","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247737 (2024)."},{"key":"4640_CR33","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247738 (2024)."},{"key":"4640_CR34","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247739 (2024)."},{"key":"4640_CR35","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247740 (2024)."},{"key":"4640_CR36","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247741 (2024)."},{"key":"4640_CR37","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247742 (2024)."},{"key":"4640_CR38","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247743 (2024)."},{"key":"4640_CR39","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247744 (2024)."},{"key":"4640_CR40","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247745 (2024)."},{"key":"4640_CR41","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247746 (2024)."},{"key":"4640_CR42","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247747 (2024)."},{"key":"4640_CR43","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247748 (2024)."},{"key":"4640_CR44","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247749 (2024)."},{"key":"4640_CR45","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247750 (2024)."},{"key":"4640_CR46","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247751 (2024)."},{"key":"4640_CR47","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247752 (2024)."},{"key":"4640_CR48","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247753 (2024)."},{"key":"4640_CR49","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247754 (2024)."},{"key":"4640_CR50","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247755 (2024)."},{"key":"4640_CR51","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247756 (2024)."},{"key":"4640_CR52","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247757 (2024)."},{"key":"4640_CR53","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247758 (2024)."},{"key":"4640_CR54","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247759 (2024)."},{"key":"4640_CR55","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247760 (2024)."},{"key":"4640_CR56","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247761 (2024)."},{"key":"4640_CR57","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247762 (2024)."},{"key":"4640_CR58","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247763 (2024)."},{"key":"4640_CR59","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247764 (2024)."},{"key":"4640_CR60","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247765 (2024)."},{"key":"4640_CR61","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247766 (2024)."},{"key":"4640_CR62","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247767 (2024)."},{"key":"4640_CR63","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247768 (2024)."},{"key":"4640_CR64","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247769 (2024)."},{"key":"4640_CR65","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247770 (2024)."},{"key":"4640_CR66","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247771 (2024)."},{"key":"4640_CR67","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247772 (2024)."},{"key":"4640_CR68","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247773 (2024)."},{"key":"4640_CR69","year":"2024","unstructured":"NCBI Sequence Read Archive https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247774 (2024)."},{"key":"4640_CR70","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.25684446","author":"C Boschiero","year":"2024","unstructured":"Boschiero, C. et al. Mapping summary statistics of 45 samples from cattle abomasal fundic and pyloric tissues. Figshare https:\/\/doi.org\/10.6084\/m9.figshare.25684446 (2024)."},{"key":"4640_CR71","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.25684455","author":"C Boschiero","year":"2024","unstructured":"Boschiero, C. et al. Time specificity gene list with Tau index (\u03c4) in cattle abomasal fundic and pyloric tissues considering four time points (3\u20135, 7\u20139, 10, and 21 dpi) vs. 0 dpi (control). Figshare https:\/\/doi.org\/10.6084\/m9.figshare.25684455 (2024)."},{"key":"4640_CR72","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.25684452","author":"C Boschiero","year":"2024","unstructured":"Boschiero, C. et al. Enriched GO pathways (FDR <0.05) for time-specific genes (\u03c4 \u2265 0.9) in cattle abomasal fundic and pyloric tissues. Figshare https:\/\/doi.org\/10.6084\/m9.figshare.25684452 (2024)."},{"key":"4640_CR73","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.25684449","author":"C Boschiero","year":"2024","unstructured":"Boschiero, C. et al. List of expressed genes present in four clusters for each cattle abomasal tissue (fundic and pyloric) with significant changes in expression over time. Figshare https:\/\/doi.org\/10.6084\/m9.figshare.25684449 (2024)."},{"key":"4640_CR74","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.25686084","author":"C Boschiero","year":"2024","unstructured":"Boschiero, C. et al. Enriched GO pathways (FDR <0.05) of expressed genes present in four clusters for each cattle abomasal tissue (fundic and pyloric) with significant changes in expression over time. Figshare https:\/\/doi.org\/10.6084\/m9.figshare.25686084 (2024)."}],"container-title":["Scientific Data"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41597-025-04640-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41597-025-04640-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41597-025-04640-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T15:19:23Z","timestamp":1740151163000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41597-025-04640-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,21]]},"references-count":74,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["4640"],"URL":"https:\/\/doi.org\/10.1038\/s41597-025-04640-6","relation":{"references":[{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247730","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247731","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247732","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247733","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247734","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247735","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247736","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247737","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247738","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247739","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247740","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247741","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247742","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247743","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247744","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247745","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247746","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247747","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247748","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247749","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247750","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247751","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247752","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247753","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247754","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247755","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247756","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247757","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247758","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247759","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247760","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247761","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247762","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247763","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247764","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247765","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247766","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247767","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247768","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247769","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247770","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247771","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247772","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247773","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/identifiers.org\/ncbi\/insdc.sra:SRR25247774","asserted-by":"subject"},{"id-type":"doi","id":"10.6084\/m9.figshare.25684446","asserted-by":"subject"},{"id-type":"doi","id":"10.6084\/m9.figshare.25684455","asserted-by":"subject"},{"id-type":"doi","id":"10.6084\/m9.figshare.25684452","asserted-by":"subject"},{"id-type":"doi","id":"10.6084\/m9.figshare.25684449","asserted-by":"subject"},{"id-type":"doi","id":"10.6084\/m9.figshare.25686084","asserted-by":"subject"}]},"ISSN":["2052-4463"],"issn-type":[{"value":"2052-4463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,21]]},"assertion":[{"value":"27 September 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"315"}}