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Integrated Genomic Profiling (IGP) utilising DNA\/RNA with tumour\/normal comparisons in a Clinical Laboratory Improvement Amendments (CLIA) compliant setting enables a single assay to provide: therapeutic target prioritisation, novel target discovery\/application and comprehensive germline assessment. A prospective study in 35 advanced\/refractory cancer patients was conducted using CLIA-compliant IGP. Feasibility was assessed by estimating time to results (TTR), prioritising\/assigning putative therapeutic targets, assessing drug access, ascertaining germline alterations, and assessing patient preferences\/perspectives on data use\/reporting. Therapeutic targets were identified using biointelligence\/pathway analyses and interpreted by a Genomic Tumour Board. Seventy-five percent of cases harboured 1\u20133 therapeutically targetable mutations\/case (median 79 mutations of potential functional significance\/case). Median time to CLIA-validated results was 116 days with CLIA-validation of targets achieved in 21\/22 patients. IGP directed treatment was instituted in 13 patients utilising on\/off label FDA approved drugs (n\u2009=\u20099), clinical trials (n\u2009=\u20093) and single patient IND (n\u2009=\u20091). Preliminary clinical efficacy was noted in five patients (two partial response, three stable disease). Although barriers to broader application exist, including the need for wider availability of therapies, IGP in a CLIA-framework is feasible and valuable in selection\/prioritisation of anti-cancer therapeutic targets.<\/jats:p>","DOI":"10.1038\/s41598-016-0021-4","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T14:51:27Z","timestamp":1482159087000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Clinical Implementation of Integrated Genomic Profiling in Patients with Advanced Cancers"],"prefix":"10.1038","volume":"6","author":[{"given":"Mitesh J.","family":"Borad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan B.","family":"Egan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel 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