{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T12:54:28Z","timestamp":1773665668425,"version":"3.50.1"},"reference-count":64,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T00:00:00Z","timestamp":1491955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T00:00:00Z","timestamp":1491955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Artificial plasma expanders (PEs) are widely used in modern transfusion medicine. PEs do not contain components of the coagulation system, so their infusion in large volumes causes haemodilution and affects haemostasis. However, the existing information on this effect is contradictory. We studied the effect of the very process of plasma dilution on coagulation and tested the hypothesis that moderate dilution with a PE should accelerate clotting owing to a decrease in concentration of coagulation inhibitors. The standard clotting times, a thrombin generation test, and the spatial rate of clot growth (test of thrombodynamics) were used to assess donor plasma diluted<jats:italic>in vitro<\/jats:italic>with various PEs. The pH value and Ca<jats:sup>+2<\/jats:sup>concentration were maintained strictly constant in all samples. The effect of thrombin inhibitors on dilution-induced hypercoagulation was also examined. It was shown that coagulation was enhanced in plasma diluted up to 2.0\u20132.5-fold with any PE. This enhancement was due to the dilution of coagulation inhibitors in plasma. Their addition to plasma or PE could partially prevent the hypercoagulation shift.<\/jats:p>","DOI":"10.1038\/s41598-017-00927-w","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T16:10:41Z","timestamp":1491495041000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5948-3444","authenticated-orcid":false,"given":"Elena I.","family":"Sinauridze","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander S.","family":"Gorbatenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena A.","family":"Seregina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena N.","family":"Lipets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fazoil I.","family":"Ataullakhanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,4,12]]},"reference":[{"key":"927_CR1","unstructured":"Volume replacement. In Alternatives to Blood Transfusion in Transfusion Medicine 2nd edn (Maniatis, A., Van der Linden, P., Hardy, J.-F. eds), Part 3 83\u2013202 (Blackwell Publishing Ltd, Oxford, UK, 2011)."},{"issue":"61\u201362","key":"927_CR2","first-page":"67","volume":"95","author":"GR Phillips","year":"1994","unstructured":"Phillips, G. R., Kauder, D. R. & Schwab, C. W. Massive blood loss in trauma patients. The benefits and dangers of transfusion therapy. Postgrad. Med. 95 61\u201372\u00a0 (1994).","journal-title":"The benefits and dangers of transfusion therapy. Postgrad. Med"},{"key":"927_CR3","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1097\/00044067-200205000-00005","volume":"13","author":"LA Lapointe","year":"2002","unstructured":"Lapointe, L. A. & Von Rueden, K. T. Coagulopathies in trauma patients. AACN Clinical Issues 13, 192\u2013203, doi:10.1097\/00044067-200205000-00005 (2002).","journal-title":"AACN Clinical Issues"},{"key":"927_CR4","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1007\/BF03018233","volume":"51","author":"JF Hardy","year":"2004","unstructured":"Hardy, J. F., De, M. P. & Samama, M. Groupe d\u2019int\u00e9r\u00eat en H\u00e9mostase P\u00e9riop\u00e9ratoire. Massive transfusion and coagulopathy: pathophysiology and implications for clinical management. Can. J. Anaesth. 51, 293\u2013310, doi:10.1007\/BF03018233 (2004).","journal-title":"Can. J. Anaesth."},{"key":"927_CR5","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1097\/00000658-197111000-00010","volume":"174","author":"RD Miller","year":"1971","unstructured":"Miller, R. D., Robbins, T. O., Tong, M. J. & Barton, S. L. Coagulation defects associated with massive blood transfusions. Ann. Surg. 174, 794\u2013801, doi:10.1097\/00000658-197111000-00010 (1971).","journal-title":"Ann. Surg."},{"key":"927_CR6","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1213\/01.ANE.0000052711.68903.5D","volume":"96","author":"K Singbartl","year":"2003","unstructured":"Singbartl, K. et al. Hemostasis and hemodilution: mathematical guide for clinical practice. Anesth. Analg. 96, 929\u2013935, doi:10.1213\/01.ANE.0000052711.68903.5D (2003).","journal-title":"Anesth. Analg."},{"issue":"4 Pt. 2","key":"927_CR7","first-page":"H1239","volume":"264","author":"WS Uijttewaal","year":"1993","unstructured":"Uijttewaal, W. S., Nijhof, E. J., Bronkhorst, P. J., Den Hartog, E. & Heethaar, R. M. Near-wall excess of platelets induced by lateral migration of erythrocytes in flowing blood. Am. J. Physiol. 264(4 Pt. 2), H1239\u2013H1244 (1993).","journal-title":"Am. J. Physiol."},{"key":"927_CR8","doi-asserted-by":"publisher","first-page":"1454","DOI":"10.1097\/00000542-199905000-00031","volume":"90","author":"B Quaknine-Orlando","year":"1999","unstructured":"Quaknine-Orlando, B. et al. Role of the hematocrit in a rabbit model of arterial thrombosis and bleeding. Anesthesiology 90, 1454\u20131461, doi:10.1097\/00000542-199905000-00031 (1999).","journal-title":"Anesthesiology"},{"key":"927_CR9","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1172\/JCI115032","volume":"87","author":"MT Santos","year":"1991","unstructured":"Santos, M. T. et al. Enhancement of platelet reactivity and modulation of eicosanoid production by intact erythrocytes. A new approach to platelet activation and recruitment. J. Clin. Invest. 87, 571\u2013580, doi:10.1172\/JCI115032 (1991).","journal-title":"J. Clin. Invest."},{"key":"927_CR10","first-page":"63","volume":"95","author":"MT Santos","year":"1997","unstructured":"Santos, M. T. et al. Prothrombotic effects of erythrocytes on platelet reactivity. Reduction by aspirin. Circulation 95, 63\u201368 (1997).","journal-title":"Reduction by aspirin. Circulation"},{"key":"927_CR11","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/S0022-5223(19)34842-1","volume":"104","author":"CR Valeri","year":"1992","unstructured":"Valeri, C. R. et al. Effect of skin temperature on platelet function in patients undergoing extacorporeal bypass. J. Thorac. Cardiovasc. Surg. 104, 108\u2013116 (1992).","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"927_CR12","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/S0002-9610(05)81018-9","volume":"160","author":"A Ferrara","year":"1990","unstructured":"Ferrara, A., MacArthur, J. D., Wright, H. K., Modlin, I. M. & McMillen, M. A. Hypothermia and acidosis worsen coagulopathy in the patient requiring massive transfusion. Am. J. Surg. 160, 515\u2013518, doi:10.1016\/S0002-9610(05)81018-9 (1990).","journal-title":"Am. J. Surg."},{"key":"927_CR13","doi-asserted-by":"publisher","unstructured":"Cosgriff, N. et al. Predicting life-threatening coagulopathy in the massive transfused trauma patient: hypothermia and acidoses revisited. J. Trauma 42, 857\u2013861, discussion 861-862, doi:10.1097\/00005373-199705000-00016 (1997).","DOI":"10.1097\/00005373-199705000-00016"},{"key":"927_CR14","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1055\/s-0037-1615025","volume":"79","author":"S Gando","year":"1998","unstructured":"Gando, S., Nanzaki, S., Sasaki, S. & Kemmotsu, O. Significant correlations between tissue factor and thrombin markers in trauma and septic patients with disseminated intravascular coagulation. Thromb. Haemost. 79, 1111\u20131115 (1998).","journal-title":"Thromb. Haemost."},{"key":"927_CR15","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1309\/AJCP3WPOYSKK6BFE","volume":"134","author":"WL Chandler","year":"2010","unstructured":"Chandler, W. L. Procoagulant activity in trauma patients. Am. J. Clin. Pathol. 134, 90\u201396, doi:10.1309\/AJCP3WPOYSKK6BFE (2010).","journal-title":"Am. J. Clin. Pathol."},{"key":"927_CR16","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1002\/bjs.7770","volume":"99","author":"B Sorensen","year":"2012","unstructured":"Sorensen, B. & Fries, D. Emerging treatment strategies for trauma-induced coagulopathy. Br. J. Surg. 99 (Suppl. 1), 40\u201350, doi:10.1002\/bjs.7770 (2012).","journal-title":"Br. J. Surg."},{"key":"927_CR17","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1097\/TA.0b013e31821266e0","volume":"70","author":"BH Shaz","year":"2011","unstructured":"Shaz, B. H., Winkler, A. M., James, A. B., Hillyer, C. D. & MacLeod, J. B. Pathophysiology of early trauma-induced coagulopathy: emerging evidence for hemodilution and coagulation factor depletion. J. Trauma 70, 1401\u20131407, doi:10.1097\/TA.0b013e31821266e0 (2011).","journal-title":"J. Trauma"},{"key":"927_CR18","doi-asserted-by":"publisher","first-page":"952","DOI":"10.1017\/S0022215111000879","volume":"125","author":"G Kontorinis","year":"2011","unstructured":"Kontorinis, G. & Schwab, B. Significance of advanced haemostasis investigation in recurrent, severe post-tonsillectomy bleeding. J. Laryngol. Otol. 125, 952\u2013957, doi:10.1017\/S0022215111000879 (2011).","journal-title":"J. Laryngol. Otol."},{"key":"927_CR19","doi-asserted-by":"crossref","unstructured":"Scalea, T. M. Hemostatic resuscitation for acute traumatic coagulopathy. Scand. J. Trauma Resusc. Emerg. Med. 19, 2 Available at: http:\/\/www.sjtrem.com\/content\/19\/1\/2 (Accessed: 12 March, 2017) (2011).","DOI":"10.1186\/1757-7241-19-2"},{"key":"927_CR20","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1097\/00003246-198505000-00003","volume":"13","author":"JR Hewson","year":"1985","unstructured":"Hewson, J. R. et al. Coagulopathy related to dilution and hypotension during massive transfusion. Crit. Care Med. 13, 387\u2013391, doi:10.1097\/00003246-198505000-00003 (1985).","journal-title":"Crit. Care Med."},{"key":"927_CR21","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1093\/bja\/80.5.612","volume":"80","author":"TG Ruttmann","year":"1998","unstructured":"Ruttmann, T. G., James, M. F. & Aronson, I. In vivo investigation into the effects of haemodilution with hydroxyethyl starch (200\/0.5) and normal saline on coagulation. Br. J. Anaesth. 80, 612\u2013616, doi:10.1093\/bja\/80.5.612 (1998).","journal-title":"Br. J. Anaesth."},{"key":"927_CR22","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1002\/bjs.1800671004","volume":"67","author":"SB Janvrin","year":"1980","unstructured":"Janvrin, S. B., Davies, G. & Greenhalgh, R. M. Postoperative deep vein thrombosis caused by intravenous fluids during surgery. Br. J. Surg. 67, 690\u2013693, doi:10.1002\/bjs.1800671004 (1980).","journal-title":"Br. J. Surg."},{"key":"927_CR23","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1213\/00000539-198709000-00009","volume":"66","author":"KJ Tuman","year":"1987","unstructured":"Tuman, K. J., Spiess, B. D., McCarthy, R. J. & Ivankovich, A. D. Effects of progressive blood loss on coagulation as measured by thrombelastography. Anesth. Analg. 66, 856\u2013863, doi:10.1213\/00000539-198709000-00009 (1987).","journal-title":"Anesth. Analg."},{"key":"927_CR24","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1093\/bja\/88.4.475","volume":"88","author":"KFJ Ng","year":"2002","unstructured":"Ng, K. F. J., Lam, C. C. K. & Chan, L. C. In vivo effect of haemodilution with saline on coagulation: a randomized controlled trial. Br. J. Anaesth 88, 475\u2013480, doi:10.1093\/bja\/88.4.475 (2002).","journal-title":"Br. J. Anaesth"},{"key":"927_CR25","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1111\/j.1365-2141.1987.tb02359.x","volume":"67","author":"D Ciavarella","year":"1987","unstructured":"Ciavarella, D. et al. Clotting factor levels and the risk of diffuse microvascular bleeding in the massively transfused patient. Br. J. Haematol. 67, 365\u2013368, doi:10.1111\/j.1365-2141.1987.tb02359.x (1987).","journal-title":"Br. J. Haematol."},{"key":"927_CR26","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1097\/00000658-197907000-00020","volume":"190","author":"RB Counts","year":"1979","unstructured":"Counts, R. B. et al. Hemostasis in massively transfused trauma patients. Ann. Surg. 190, 91\u201399, doi:10.1097\/00000658-197907000-00020 (1979).","journal-title":"Ann. Surg."},{"key":"927_CR27","doi-asserted-by":"publisher","unstructured":"Hellstern, P. & Haubelt, H. Indications for plasma in massive transfusion. Thromb. Res. 107 (Suppl. 1), S19\u2013S22, doi:10.1016\/S0049-3848(02)00147-0 (2002).","DOI":"10.1016\/S0049-3848(02)00147-0"},{"key":"927_CR28","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1182\/blood.V66.5.1189.1189","volume":"66","author":"J Jesty","year":"1985","unstructured":"Jesty, J. The kinetics of inhibition of thrombin by antithrombin in the presence of components of the hemostatic system. Blood 66, 1189\u20131195 (1985).","journal-title":"Blood"},{"key":"927_CR29","doi-asserted-by":"crossref","first-page":"10313","DOI":"10.1016\/S0021-9258(18)67526-X","volume":"261","author":"J Jesty","year":"1986","unstructured":"Jesty, J. The kinetics of inhibition of \u03b1-thrombin in human plasma. J. Biol. Chem. 261, 10313\u201310318 (1986).","journal-title":"J. Biol. Chem."},{"key":"927_CR30","first-page":"360","volume":"81","author":"ST Hiippala","year":"1995","unstructured":"Hiippala, S. T., Myllyl\u00e4, G. J. & Vahtera, E. M. Hemostatic factors and replacement of major blood loss with plasma-poor red cell concentrates. Anesth. Analg. 81, 360\u2013365 (1995).","journal-title":"Anesth. Analg."},{"key":"927_CR31","first-page":"336","volume":"80","author":"DJ Murray","year":"1995","unstructured":"Murray, D. J., Pennell, B. J., Weinstein, S. L. & Olson, J. D. Packed red cells in acute blood loss: dilutional coagulopathy as a cause of surgical bleeding. Anesth. Analg. 80, 336\u2013342 (1995).","journal-title":"Anesth. Analg."},{"key":"927_CR32","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1111\/j.1423-0410.1982.tb01080.x","volume":"42","author":"PM Mannucci","year":"1982","unstructured":"Mannucci, P. M., Federici, A. B. & Sirchia, G. Hemostasis testing during massive blood replacement. A study of 172 cases. Vox Sang 42, 113\u2013123, http:\/\/onlinelibrary.wiley.com.sci-hub,io\/doi\/10.1111\/vox.1982.42.issue-3\/issuetoc (1982).","journal-title":"Vox Sang"},{"key":"927_CR33","first-page":"125","volume":"47","author":"CE Lucas","year":"1981","unstructured":"Lucas, C. E. & Ledgerwood, A. M. Clinical significance of altered coagulation tests after massive transfusion for trauma. Am. Surg. 47, 125\u2013130 (1981).","journal-title":"Am. Surg."},{"key":"927_CR34","first-page":"455","volume":"169","author":"RL Simmons","year":"1969","unstructured":"Simmons, R. L. et al. Coagulation disorders in combat casualties. I. Acute changes after wounding. II. Effects of massive transfusion. III. Post-resuscitative changes. Ann. Surg. 169, 455\u2013482 (1969).","journal-title":"Post-resuscitative changes. Ann. Surg."},{"key":"927_CR35","unstructured":"Sinauridze, E. I. et al. New anticoagulant compounds, pharmaceutical compositions on their basis to tread thrombotic conditions, and plasma-substituting solution to correct hypercoagulation defects of hemodilution. US Patent \u2116 8426433 (23.04.2013). Patent holder: Bionika, Moscow, Russia."},{"key":"927_CR36","doi-asserted-by":"publisher","unstructured":"Sinauridze, E. I. et al. New synthetic thrombin inhibitors: Molecular design and experimental verification. PLoS ONE 6 ( 5 ), e19969 (2011) doi: 10.1371\/journal.pone.0019969 (Accessed: 12 March, 2017).","DOI":"10.1371\/journal.pone.0019969"},{"key":"927_CR37","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1111\/j.1538-7933.2003.00617.x","volume":"2","author":"GA Allen","year":"2004","unstructured":"Allen, G. A. et al. Impact of procoagulant concentration on rate, peak and total thrombin generation in a model system. J. Thromb. Haemost. 2, 402\u2013413, doi:10.1111\/jth.2004.2.issue-3 (2004).","journal-title":"J. Thromb. Haemost."},{"key":"927_CR38","unstructured":"Michetti, C. P. & Fakhry, S. M. Management of coagulation disorders in the surgical intensive care unit. In Current Therapy of Trauma and Surgical Critical Care 2nd edn (eds Asensio, J. A., Trunkey, D. D.) 650\u2013658 (Elsevier Health Sciences, 2015)."},{"key":"927_CR39","doi-asserted-by":"publisher","first-page":"S90","DOI":"10.1097\/00003246-200107001-00028","volume":"29","author":"M Levi","year":"2001","unstructured":"Levi, M., de Jonge, E. & van der Poll, T. Rationale for restoration of physiological anticoagulant pathways in patients with sepsis and disseminated intravascular coagulation. Crit. Care Med. 29, S90\u2013S94, doi:10.1097\/00003246-200107001-00028 (2001).","journal-title":"Crit. Care Med."},{"key":"927_CR40","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1046\/j.1365-2044.2002.02782_1.x","volume":"57","author":"K Ekseth","year":"2002","unstructured":"Ekseth, K., Abildgaard, L., Vegfors, M., Berg-Johnsen, J. & Engdahl, O. The in vitro effects of crystalloids and colloids on coagulation. Anaesthesia 57, 1102\u20131108, doi:10.1046\/j.1365-2044.2002.02782_1.x (2002).","journal-title":"Anaesthesia"},{"key":"927_CR41","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1093\/bja\/76.3.412","volume":"76","author":"TG Ruttmann","year":"1996","unstructured":"Ruttmann, T. G., James, M. F. & Viljoen, J. F. Haemodilution induces a hypercoagulable state. Br. J. Anaesth. 76, 412\u2013414, doi:10.1093\/bja\/76.3.412 (1996).","journal-title":"Br. J. Anaesth."},{"key":"927_CR42","first-page":"165","volume":"101","author":"E De Smedt","year":"2009","unstructured":"De Smedt, E., Wagenvoord, R. & Hemker, H. C. The technique of measuring thrombin generation with fluorogenic substrates: 3. The effects of sample dilution. Thromb. Haemost 101, 165\u2013170 (2009).","journal-title":"The effects of sample dilution. Thromb. Haemost"},{"key":"927_CR43","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1055\/s-2006-939552","volume":"32","author":"HR Roberts","year":"2006","unstructured":"Roberts, H. R., Hoffman, M. & Monroe, D. M. A cell-based model of thrombin generation. Semin. Thromb. Hemost. 32, 32\u201338, doi:10.1055\/s-2006-939552 (2006).","journal-title":"Semin. Thromb. Hemost."},{"key":"927_CR44","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/0925-4439(94)90086-8","volume":"1227","author":"M Kalafatis","year":"1994","unstructured":"Kalafatis, M., Swords, N. A., Rand, M. D. & Mann, K. G. Membrane-dependent reactions in blood coagulation: role of the vitamin K-dependent enzyme complexes. Biochim. Biophys. Acta 1227, 113\u2013129, doi:10.1016\/0925-4439(94)90086-8 (1994).","journal-title":"Biochim. Biophys. Acta"},{"key":"927_CR45","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/j.1423-0410.2000.tb00058.x","volume":"78","author":"P Rebulla","year":"2000","unstructured":"Rebulla, P. Trigger for platelet transfusion. Vox Sang. 78 (Suppl. 2), 179\u2013182 (2000).","journal-title":"Vox Sang."},{"key":"927_CR46","doi-asserted-by":"publisher","unstructured":"Hiippala, S. Replacement of massive blood loss. Vox Sang. 74 (Suppl. 2), 399\u2013407, doi:10.1111\/vox.1998.74.issue-S2 (1998).","DOI":"10.1111\/vox.1998.74.issue-S2"},{"key":"927_CR47","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1097\/00000658-197111000-00010","volume":"174","author":"RD Miller","year":"1971","unstructured":"Miller, R. D., Robbins, T. O., Tong, M. J. & Barton, S. L. Coagulation defects associated with massive blood transfusions. Ann. Surg. 174, 794\u2013801, doi:10.1097\/00000658-197111000-00010 (1971).","journal-title":"Ann. Surg."},{"key":"927_CR48","first-page":"720","volume":"6","author":"LM Tocantins","year":"1951","unstructured":"Tocantins, L. M., Carroll, R. T. & Holburn, R. H. The clot accelerating effect of dilution on blood and plasma. Relation to the mechanism of coagulation of normal and hemophilic plasma. Blood 6, 720\u2013739 (1951).","journal-title":"Relation to the mechanism of coagulation of normal and hemophilic plasma. Blood"},{"key":"927_CR49","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1160\/TH06-06-0313","volume":"97","author":"EI Sinauridze","year":"2007","unstructured":"Sinauridze, E. I. et al. Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets. Thromb. Haemost. 97, 425\u2013434, doi:10.1160\/TH06-06-0313 (2007).","journal-title":"Thromb. Haemost."},{"key":"927_CR50","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1093\/bja\/69.3.307","volume":"69","author":"SV Mallett","year":"1992","unstructured":"Mallett, S. V. & Cox, D. J. Thrombelastography. Br. J. Anaesth. 69, 307\u2013313, doi:10.1093\/bja\/69.3.307 (1992).","journal-title":"Br. J. Anaesth."},{"key":"927_CR51","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1055\/s-0037-1614109","volume":"84","author":"HC Hemker","year":"2000","unstructured":"Hemker, H. C. & Beguin, S. Phenotyping the clotting system. Thromb. Haemost. 84, 747\u2013751 (2000).","journal-title":"Thromb. Haemost."},{"key":"927_CR52","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1177\/0310057X0102900507","volume":"29","author":"TG Ruttmann","year":"2001","unstructured":"Ruttmann, T. G., James, M. F. M. & Lombard, E. H. Haemodilution-induced enhancement of coagulation is attenuated iv vitro by restoring antithrombin III to pre-dilution concentrations. Anesth. Intensive Care 29, 489\u2013493 (2001).","journal-title":"Anesth. Intensive Care"},{"key":"927_CR53","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1093\/bja\/88.4.470","volume":"88","author":"TG Ruttmann","year":"2002","unstructured":"Ruttmann, T. G. Haemodilution enhances coagulation. Br. J. Anaesth 88, 470\u2013472, doi:10.1093\/bja\/88.4.470 (2002).","journal-title":"Br. J. Anaesth"},{"key":"927_CR54","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1097\/00000539-200003000-00008","volume":"90","author":"VG Nielsen","year":"2000","unstructured":"Nielsen, V. G. & Baird, M. S. Extreme hemodilution in rabbits: an in vitro and in vivo thrombelastographic analysis. Anesth. Analg. 90, 541\u2013545, doi:10.1097\/00000539-200003000-00008 (2000).","journal-title":"Anesth. Analg."},{"key":"927_CR55","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1097\/00001721-200401000-00009","volume":"15","author":"VG Nielsen","year":"2004","unstructured":"Nielsen, V. G. Hemodilution with lactated Ringer\u2019s solution causes hypercoagulability in rabbits. Blood Coagul. Fibrinolysis 15, 55\u201359, doi:10.1097\/00001721-200401000-00009 (2004).","journal-title":"Blood Coagul. Fibrinolysis"},{"key":"927_CR56","doi-asserted-by":"publisher","first-page":"1587","DOI":"10.1213\/01.ANE.0000136843.58799.AB","volume":"99","author":"VG Nielsen","year":"2004","unstructured":"Nielsen, V. G., Lyerly, R. T. 3rd & Gurley, W. Q. The effect of dilution on plasma coagulation kinetics determined by thrombelastography is dependent on antithrombin activity and mode of activation. Anesth. Analg. 99, 1587\u20131592, doi:10.1213\/01.ANE.0000136843.58799.AB (2004).","journal-title":"Anesth. Analg."},{"key":"927_CR57","doi-asserted-by":"crossref","unstructured":"Hemker, H. C. et al. The calibrated automated thrombogram (CAT): a universal routine test for hyper- and hypocoagulability. Pathophysiol. Haemost. Thromb. 32, 249\u2013253, 73575 (2002).","DOI":"10.1159\/000073575"},{"key":"927_CR58","doi-asserted-by":"crossref","unstructured":"Hemker, H. C. et al. Calibrated automated thrombin generation measurement in clotting plasma. Pathophysiol. Haemost. Thromb. 33, 4\u201315, 71636 (2003).","DOI":"10.1159\/000071636"},{"key":"927_CR59","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1111\/j.1432-1033.1988.tb13849.x","volume":"172","author":"S Kawabata","year":"1988","unstructured":"Kawabata, S. et al. Highly sensitive peptide-4-methylcoumaryl-7-amide substrates for blood-clotting proteases and trypsin. Eur. J. Biochem. 172, 17\u201325, doi:10.1111\/ejb.1988.172.issue-1 (1988).","journal-title":"Eur. J. Biochem."},{"key":"927_CR60","doi-asserted-by":"publisher","unstructured":"Gribkova, I. et al. The modification of the thrombin generation test for the clinical assessment of dabigatran etexilate efficiency. Sci. Rep. 6, 29242, doi: 10.1038\/srep29242 (2016) (Accessed: 12 March, 2017).","DOI":"10.1038\/srep29242"},{"key":"927_CR61","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/S0304-4165(02)00278-7","volume":"1572","author":"MV Ovanesov","year":"2002","unstructured":"Ovanesov, M. V. et al. Hemophilia A and B are associated with abnormal spatial dynamics of clot growth. Biochim. Biophys. Acta 1572, 45\u201357, doi:10.1016\/S0304-4165(02)00278-7 (2002).","journal-title":"Biochim. Biophys. Acta"},{"key":"927_CR62","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1016\/j.thromres.2013.12.014","volume":"133","author":"NM Dashkevich","year":"2014","unstructured":"Dashkevich, N. M. et al. Effect of pre-analytical conditions on the thrombodynamics assay. Thromb. Res. 133, 472\u2013476, doi:10.1016\/j.thromres.2013.12.014 (2014).","journal-title":"Thromb. Res."},{"key":"927_CR63","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1097\/MBC.0b013e328352e90e","volume":"23","author":"NP Soshitova","year":"2012","unstructured":"Soshitova, N. P. et al. Predicting prothrombotic tendencies in sepsis using spatial clot growth dynamics. Blood Coagul. Fibrinolysis 23, 498\u2013507, doi:10.1097\/MBC.0b013e328352e90e (2012).","journal-title":"Blood Coagul. Fibrinolysis"},{"key":"927_CR64","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1134\/S0006297910060088","volume":"75","author":"OA Fadeeva","year":"2010","unstructured":"Fadeeva, O. A. et al. Thromboplastin immobilized on polystyrene surface exhibits kinetic characteristics close to those for the native protein and activates in vitro blood coagulation similarly to thromboplastin on fibroblasts. Biochemistry (Mosc.) 75, 734\u2013743, doi:10.1134\/S0006297910060088 (2010).","journal-title":"Biochemistry (Mosc.)"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-00927-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-00927-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-00927-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T21:12:46Z","timestamp":1750194766000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-00927-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,12]]},"references-count":64,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["927"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-00927-w","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,12]]},"assertion":[{"value":"10 June 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 March 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 April 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"843"}}