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Both PCR-based and MLVA methods are limited in discriminating <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> strains. A new MLVA-13Bc method for <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a <jats:italic>B<\/jats:italic>. <jats:italic>canis<\/jats:italic> outbreak.<\/jats:p>","DOI":"10.1038\/s41598-017-01114-7","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T14:23:20Z","timestamp":1492093400000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay"],"prefix":"10.1038","volume":"7","author":[{"given":"Yi","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Poulsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russell","family":"Ransburgh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoyan","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanyan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0021-3036","authenticated-orcid":false,"given":"Jianfa","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,4,21]]},"reference":[{"key":"1114_CR1","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.anireprosci.2004.05.005","volume":"82\u201383","author":"MM Wanke","year":"2004","unstructured":"Wanke, M. 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