{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T16:30:16Z","timestamp":1782491416990,"version":"3.54.5"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Grass carp (<jats:italic>Ctenopharyngodon idella<\/jats:italic>) is an important economic species in freshwater aquaculture and its industry has been confined due to variety degeneration and frequent diseases. Marker-assisted selection is a feasible method for selective breeding of new varieties. Transcriptome data have greatly facilitated high-throughput single nucleotide polymorphism (SNP) marker discovery and phenotype association study. In this study, we gained a total of 25,981 and 5,775 high quality SNPs in two transcriptomes from individuals and cell lines, respectively. Comparative transcriptome analysis identified 413 and 832 grass carp reovirus (GCRV)-resistant-association SNPs as well as 1,381 and 1,606 GCRV-susceptible-association SNPs in individuals and cell lines, respectively. Integrated analysis indicated 22 genes with single SNP share common resistant\/susceptible traits in two transcriptomes. Furthermore, we infected grass carp with GCRV, genotyping and association analyses were performed, and 9 in 22 SNPs were confirmed by PCR-RFLP. Meanwhile, mRNA expression profiles of 6 genes containing confirmed SNPs were examined by qRT-PCR. The results demonstrated that mRNA expressions were significant differences in resistant\/susceptible individuals and cell lines. The present study develops an important strategy for high throughput screening of phenotype association genetic markers and the results will serve in grass carp breeding for GCRV resistance.<\/jats:p>","DOI":"10.1038\/s41598-017-01338-7","type":"journal-article","created":{"date-parts":[[2017,4,20]],"date-time":"2017-04-20T10:22:31Z","timestamp":1492683751000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Large-scale SNP screenings identify markers linked with GCRV resistant traits through transcriptomes of individuals and cell lines in Ctenopharyngodon idella"],"prefix":"10.1038","volume":"7","author":[{"given":"Zhiwei","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanyuan","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueying","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1340-6144","authenticated-orcid":false,"given":"Jianguo","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"1338_CR1","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1038\/ng.3280","volume":"47","author":"Y Wang","year":"2015","unstructured":"Wang, Y. et al. The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation. Nat. genet. \n                           47, 625\u2013631, doi:10.1038\/ng.3280 (2015).","journal-title":"Nat. genet."},{"key":"1338_CR2","doi-asserted-by":"publisher","first-page":"35433","DOI":"10.18632\/oncotarget.5945","volume":"6","author":"R Huang","year":"2015","unstructured":"Huang, R. et al. Genetic variations of body weight and GCRV resistance in a random mating population of grass carp. Oncotarget \n                           6, 35433\u201335442, doi:10.18632\/oncotarget.5945 (2015).","journal-title":"Oncotarget"},{"key":"1338_CR3","doi-asserted-by":"publisher","first-page":"670437","DOI":"10.1155\/2015\/670437","volume":"2015","author":"Y Rao","year":"2015","unstructured":"Rao, Y. & Su, J. Insights into the antiviral immunity against grass carp (Ctenopharyngodon idella) reovirus (GCRV) in grass carp. J. Immunol. Res. \n                           2015, 670437, doi:10.1155\/2015\/670437 (2015).","journal-title":"J. Immunol. Res."},{"key":"1338_CR4","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.jmb.2008.06.075","volume":"382","author":"L Cheng","year":"2008","unstructured":"Cheng, L., Fang, Q., Shah, S., Atanasov, I. C. & Zhou, Z. H. Subnanometer-resolution structures of the grass carp reovirus core and virion. J. Mol. Biol. \n                           382, 213\u2013222, doi:10.1016\/j.jmb.2008.06.075 (2008).","journal-title":"J. Mol. Biol."},{"key":"1338_CR5","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1016\/j.fsi.2008.12.007","volume":"26","author":"J Su","year":"2009","unstructured":"Su, J. et al. Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus). Fish Shellfish Immunol. \n                           26, 828\u2013835, doi:10.1016\/j.fsi.2008.12.007 (2009).","journal-title":"Fish Shellfish Immunol."},{"key":"1338_CR6","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1016\/j.dci.2013.08.022","volume":"41","author":"Q Wan","year":"2013","unstructured":"Wan, Q., Su, J., Chen, X. & Yang, C. Gene-based polymorphisms, genomic organization of interferon-beta promoter stimulator 1 (IPS-1) gene and association study with the natural resistance to grass carp reovirus in grass carp Ctenopharyngodon idella. Dev. Comp. Immunol. \n                           41, 756\u2013765, doi:10.1016\/j.dci.2013.08.022 (2013).","journal-title":"Dev. Comp. Immunol."},{"key":"1338_CR7","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.aquaculture.2007.07.210","volume":"272","author":"K Fuji","year":"2007","unstructured":"Fuji, K. et al. Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus). Aquaculture \n                           272, 291\u2013295, doi:10.1016\/j.aquaculture.2007.07.210 (2007).","journal-title":"Aquaculture"},{"key":"1338_CR8","doi-asserted-by":"publisher","first-page":"12946","DOI":"10.1038\/srep12946","volume":"5","author":"Q Wan","year":"2015","unstructured":"Wan, Q. & Su, J. Transcriptome analysis provides insights into the regulatory function of alternative splicing in antiviral immunity in grass carp (Ctenopharyngodon idella). Sci. Rep \n                           5, 12946, doi:10.1038\/srep12946 (2015).","journal-title":"Sci. Rep"},{"key":"1338_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.aquaculture.2004.05.027","volume":"238","author":"Z Liu","year":"2004","unstructured":"Liu, Z. & Cordes, J. DNA marker technologies and their applications in aquaculture genetics. Aquaculture \n                           238, 1\u201337, doi:10.1016\/j.aquaculture.2004.05.027 (2004).","journal-title":"Aquaculture"},{"key":"1338_CR10","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1126\/science.1105436","volume":"307","author":"AH David","year":"2005","unstructured":"David, A. H. et al. Whole-genome patterns of common DNA variation in three human populations. Science \n                           307, 1072\u20131079, doi:10.1126\/science.1105436 (2005).","journal-title":"Science"},{"key":"1338_CR11","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1093\/carcin\/bgs334","volume":"34","author":"EA Ruiz-Narvaez","year":"2013","unstructured":"Ruiz-Narvaez, E. A. et al. Fine-mapping of the 6q25 locus identifies a novel SNP associated with breast cancer risk in African-American women. Carcinogenesis \n                           34, 287\u2013291, doi:10.1093\/carcin\/bgs334 (2013).","journal-title":"Carcinogenesis"},{"key":"1338_CR12","doi-asserted-by":"publisher","first-page":"e1003212","DOI":"10.1371\/journal.pgen.1003212","volume":"9","author":"JC Fergus","year":"2013","unstructured":"Fergus, J. C. Genome-wide association study in BRCA1 mutation carriers identifies novel loci associated with breast and ovarian cancer risk. PLoS Genet. \n                           9, e1003212, doi:10.1371\/journal.pgen.1003212 (2013).","journal-title":"PLoS Genet."},{"key":"1338_CR13","doi-asserted-by":"publisher","first-page":"e70051","DOI":"10.1371\/journal.pone.0070051","volume":"8","author":"I Montes","year":"2013","unstructured":"Montes, I. et al. SNP discovery in European anchovy (Engraulis encrasicolus, L) by high-throughput transcriptome and genome sequencing. PLoS One \n                           8, e70051, doi:10.1371\/journal.pone.0070051 (2013).","journal-title":"PLoS One"},{"key":"1338_CR14","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1186\/s12864-015-2117-9","volume":"16","author":"HY Tsai","year":"2015","unstructured":"Tsai, H. Y. et al. Genome wide association and genomic prediction for growth traits in juvenile farmed Atlantic salmon using a high density SNP array. BMC genomics \n                           16, 969, doi:10.1186\/s12864-015-2117-9 (2015).","journal-title":"BMC genomics"},{"key":"1338_CR15","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/s11032-015-0240-6","volume":"35","author":"X Xu","year":"2015","unstructured":"Xu, X. & Bai, G. Whole-genome resequencing: changing the paradigms of SNP detection, molecular mapping and gene discovery. Mol. Breed. \n                           35, 33, doi:10.1007\/s11032-015-0240-6 (2015).","journal-title":"Mol. Breed."},{"key":"1338_CR16","doi-asserted-by":"publisher","first-page":"e87218","DOI":"10.1371\/journal.pone.0087218","volume":"9","author":"Y Yu","year":"2014","unstructured":"Yu, Y. et al. SNP discovery in the transcriptome of white pacific shrimp Litopenaeus vannamei by next generation sequencing. PLoS One \n                           9, e87218, doi:10.1371\/journal.pone.0087218 (2014).","journal-title":"PLoS One"},{"key":"1338_CR17","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li, H. et al. The sequence alignment\/map format and SAMtools. Bioinformatics \n                           25, 2078\u20132079, doi:10.1093\/bioinformatics\/btp352 (2009).","journal-title":"Bioinformatics"},{"key":"1338_CR18","doi-asserted-by":"publisher","first-page":"e58627","DOI":"10.1371\/journal.pone.0058627","volume":"8","author":"S Li","year":"2013","unstructured":"Li, S., Zhang, X., Sun, Z., Li, F. & Xiang, J. Transcriptome analysis on Chinese shrimp Fenneropenaeus chinensis during WSSV acute infection. PLoS One \n                           8, e58627, doi:10.1371\/journal.pone.0058627 (2013).","journal-title":"PLoS One"},{"key":"1338_CR19","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/1471-2164-12-53","volume":"12","author":"S Liu","year":"2011","unstructured":"Liu, S. et al. Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array. BMC genomics \n                           12, 53, doi:10.1186\/1471-2164-12-53 (2011).","journal-title":"BMC genomics"},{"key":"1338_CR20","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1007\/s11427-012-4425-5","volume":"56","author":"C Chen","year":"2013","unstructured":"Chen, C. et al. Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella. Sci. China Life Sci. \n                           56, 59\u201365, doi:10.1007\/s11427-012-4425-5 (2013).","journal-title":"Sci. China Life Sci."},{"key":"1338_CR21","doi-asserted-by":"publisher","first-page":"24984","DOI":"10.1038\/srep24984","volume":"6","author":"D Li","year":"2016","unstructured":"Li, D. et al. Gene expression analysis and SNP\/InDel discovery to investigate yield heterosis of two rubber tree F1 hybrids. Sci. Rep. \n                           6, 24984, doi:10.1038\/srep24984 (2016).","journal-title":"Sci. Rep."},{"key":"1338_CR22","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1093\/bfgp\/elt046","volume":"13","author":"HP Spaink","year":"2014","unstructured":"Spaink, H. P., Jansen, H. J. & Dirks, R. P. Advances in genomics of bony fish. Brief. Funct. Genomics \n                           13, 144\u2013156, doi:10.1093\/bfgp\/elt046 (2014).","journal-title":"Brief. Funct. Genomics"},{"key":"1338_CR23","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1038\/nbt.1883","volume":"29","author":"MG Grabherr","year":"2011","unstructured":"Grabherr, M. G. et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. biotechnol. \n                           29, 644\u2013652, doi:10.1038\/nbt.1883 (2011).","journal-title":"Nat. biotechnol."},{"key":"1338_CR24","doi-asserted-by":"publisher","first-page":"e13574","DOI":"10.1371\/journal.pone.0013574","volume":"5","author":"R Chen","year":"2010","unstructured":"Chen, R., Davydov, E. V., Sirota, M. & Butte, A. J. Non-synonymous and synonymous coding SNPs show similar likelihood and effect size of human disease association. Plos One \n                           5, e13574, doi:10.1371\/journal.pone.0013574 (2010).","journal-title":"Plos One"},{"key":"1338_CR25","doi-asserted-by":"publisher","first-page":"e1003373","DOI":"10.1371\/journal.pcbi.1003373","volume":"9","author":"T Warnecke","year":"2013","unstructured":"Warnecke, T., Becker, E. A., Facciotti, M. T., Nislow, C. & Lehner, B. Conserved substitution patterns around nucleosome footprints in eukaryotes and archaea derive from frequent nucleosome repositioning through evolution. PLoS Comput. Biol. \n                           9, e1003373, doi:10.1371\/journal.pcbi.1003373 (2013).","journal-title":"PLoS Comput. Biol."},{"key":"1338_CR26","doi-asserted-by":"publisher","first-page":"e92502","DOI":"10.1371\/journal.pone.0092502","volume":"9","author":"J Cui","year":"2014","unstructured":"Cui, J. et al. SNP discovery from transcriptome of the swimbladder of Takifugu rubripes. PLoS One \n                           9, e92502, doi:10.1371\/journal.pone.0092502 (2014).","journal-title":"PLoS One"},{"key":"1338_CR27","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.1101\/gr.4565806","volume":"16","author":"RE Mills","year":"2006","unstructured":"Mills, R. E. et al. An initial map of insertion and deletion (INDEL) variation in the human genome. Genome Res. \n                           16, 1182\u20131190, doi:10.1101\/gr.4565806 (2006).","journal-title":"Genome Res."},{"key":"1338_CR28","doi-asserted-by":"publisher","first-page":"e31613","DOI":"10.1371\/journal.pone.0031613","volume":"7","author":"C Zakas","year":"2012","unstructured":"Zakas, C., Schult, N., McHugh, D., Jones, K. L. & Wares, J. P. Transcriptome analysis and SNP development can resolve population differentiation of Streblospio benedicti, a developmentally dimorphic marine annelid. PLoS One \n                           7, e31613, doi:10.1371\/journal.pone.0031613 (2012).","journal-title":"PLoS One"},{"key":"1338_CR29","doi-asserted-by":"publisher","first-page":"e90485","DOI":"10.1371\/journal.pone.0090485","volume":"9","author":"JZ Li","year":"2014","unstructured":"Li, J. Z. et al. Comparison of Illumina and 454 deep sequencing in participants failing raltegravir-based antiretroviral therapy. PLoS One \n                           9, e90485, doi:10.1371\/journal.pone.0090485 (2014).","journal-title":"PLoS One"},{"key":"1338_CR30","doi-asserted-by":"publisher","first-page":"e58815","DOI":"10.1371\/journal.pone.0058815","volume":"8","author":"EM Quinn","year":"2013","unstructured":"Quinn, E. M. et al. Development of strategies for SNP detection in RNA-seq data: application to lymphoblastoid cell lines and evaluation using 1000 Genomes data. PLoS One \n                           8, e58815, doi:10.1371\/journal.pone.0058815 (2013).","journal-title":"PLoS One"},{"key":"1338_CR31","doi-asserted-by":"publisher","first-page":"e42089","DOI":"10.1371\/journal.pone.0042089","volume":"7","author":"SJ Helyar","year":"2012","unstructured":"Helyar, S. J. et al. SNP discovery using next generation transcriptomic sequencing in Atlantic herring (Clupea harengus). PLoS One \n                           7, e42089, doi:10.1371\/journal.pone.0042089 (2012).","journal-title":"PLoS One"},{"key":"1338_CR32","doi-asserted-by":"publisher","first-page":"3674","DOI":"10.1093\/bioinformatics\/bti610","volume":"21","author":"A Conesa","year":"2005","unstructured":"Conesa, A. et al. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics \n                           21, 3674\u20133676, doi:10.1093\/bioinformatics\/bti610 (2005).","journal-title":"Bioinformatics"},{"key":"1338_CR33","doi-asserted-by":"publisher","first-page":"3420","DOI":"10.1093\/nar\/gkn176","volume":"36","author":"S Gotz","year":"2008","unstructured":"Gotz, S. et al. High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res. \n                           36, 3420\u20133435, doi:10.1093\/nar\/gkn176 (2008).","journal-title":"Nucleic Acids Res."},{"key":"1338_CR34","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1186\/1471-2164-10-122","volume":"10","author":"K Laurila","year":"2009","unstructured":"Laurila, K. & Vihinen, M. Prediction of disease-related mutations affecting protein localization. BMC genomics \n                           10, 122, doi:10.1186\/1471-2164-10-122 (2009).","journal-title":"BMC genomics"},{"key":"1338_CR35","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1038\/msb.2009.80","volume":"5","author":"Q Zhong","year":"2009","unstructured":"Zhong, Q. et al. Edgetic perturbation models of human inherited disorders. Mol. Syst. Biol. \n                           5, 321, doi:10.1038\/msb.2009.80 (2009).","journal-title":"Mol. Syst. Biol."},{"key":"1338_CR36","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1038\/nmeth.1394","volume":"6","author":"M Dreze","year":"2009","unstructured":"Dreze, M. et al. \u2018Edgetic\u2019 perturbation of a C. elegans BCL2 ortholog. Nat. Methods \n                           6, 843\u2013849, doi:10.1038\/nmeth.1394 (2009).","journal-title":"Nat. Methods"},{"key":"1338_CR37","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1104\/pp.112.200311","volume":"160","author":"B Hartwig","year":"2012","unstructured":"Hartwig, B., James, G. V., Konrad, K., Schneeberger, K. & Turck, F. Fast isogenic mapping-by-sequencing of ethyl methanesulfonate-induced mutant bulks. Plant Physiol. \n                           160, 591\u2013600, doi:10.1104\/pp.112.200311 (2012).","journal-title":"Plant Physiol."},{"key":"1338_CR38","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1186\/s12870-016-0779-3","volume":"16","author":"W Tang","year":"2016","unstructured":"Tang, W. et al. SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice. BMC Plant Biol \n                           16, 93, doi:10.1186\/s12870-016-0779-3 (2016).","journal-title":"BMC Plant Biol"},{"key":"1338_CR39","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/nrg2398","volume":"9","author":"L Lopez-Maury","year":"2008","unstructured":"Lopez-Maury, L., Marguerat, S. & Bahler, J. Tuning gene expression to changing environments: from rapid responses to evolutionary adaptation. Nat. Rev. Genet. \n                           9, 583\u2013593, doi:10.1038\/nrg2398 (2008).","journal-title":"Nat. Rev. Genet."},{"key":"1338_CR40","doi-asserted-by":"publisher","first-page":"9033","DOI":"10.1073\/pnas.0402591101","volume":"101","author":"HB Fraser","year":"2004","unstructured":"Fraser, H. B., Hirsh, A. E., Wall, D. P. & Eisen, M. B. Coevolution of gene expression among interacting proteins. Proc. Natl. Acad. Sci. USA \n                           101, 9033\u20139038, doi:10.1073\/pnas.0402591101 (2004).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"1338_CR41","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1093\/molbev\/msu328","volume":"32","author":"EH Leder","year":"2015","unstructured":"Leder, E. H. et al. The evolution and adaptive potential of transcriptional variation in Sticklebacks-signatures of selection and widespread heritability. Mol. Biol. Evol. \n                           32, 674\u2013689, doi:10.1093\/molbev\/msu328 (2015).","journal-title":"Mol. Biol. Evol."},{"key":"1338_CR42","doi-asserted-by":"publisher","first-page":"5389","DOI":"10.1111\/j.1365-294X.2010.04934.x","volume":"19","author":"J Jeukens","year":"2010","unstructured":"Jeukens, J., Renaut, S., St-Cyr, J., Nolte, A. W. & Bernatchez, L. The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next-generation sequencing.\u00a0Mol. Ecol.\u00a019, 5389\u20135403, doi:10.1111\/j.1365-294X.2010.04934.x (2010).","journal-title":"Mol. Ecol."},{"key":"1338_CR43","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1038\/nbt.2302","volume":"30","author":"AL Harper","year":"2012","unstructured":"Harper, A. L. et al. Associative transcriptomics of traits in the polyploid crop species Brassica napus. Nat. biotechnol. \n                           30, 798\u2013802, doi:10.1038\/nbt.2302 (2012).","journal-title":"Nat. biotechnol."},{"key":"1338_CR44","doi-asserted-by":"publisher","first-page":"2310","DOI":"10.1111\/mec.13165","volume":"24","author":"P De Wit","year":"2015","unstructured":"De Wit, P., Pespeni, M. H. & Palumbi, S. R. SNP genotyping and population genomics from expressed sequences-current advances and future possibilities. Mol. Ecol. \n                           24, 2310\u20132323, doi:10.1111\/mec.13165 (2015).","journal-title":"Mol. Ecol."},{"key":"1338_CR45","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.fob.2014.01.004","volume":"4","author":"CY-Y Szeto","year":"2014","unstructured":"Szeto, C. Y.-Y. et al. Integrated mRNA and microRNA transcriptome sequencing characterizes sequence variants and mRNA-microRNA regulatory network in nasopharyngeal carcinoma model systems. FEBS Open Bio. \n                           4, 128\u2013140, doi:10.1016\/j.fob.2014.01.004 (2014).","journal-title":"FEBS Open Bio."},{"key":"1338_CR46","doi-asserted-by":"publisher","first-page":"R136","DOI":"10.1186\/gb-2013-14-12-r136","volume":"14","author":"E Loire","year":"2013","unstructured":"Loire, E. et al. Population genomics of the endangered giant Gal\u00e1pagos tortoise. Genome Biol. \n                           14, R136, doi:10.1186\/gb-2013-14-12-r136 (2013).","journal-title":"Genome Biol."},{"key":"1338_CR47","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1038\/nature13685","volume":"515","author":"J Romiguier","year":"2014","unstructured":"Romiguier, J. et al. Comparative population genomics in animals uncovers the determinants of genetic diversity. Nature \n                           515, 261\u2013263, doi:10.1038\/nature13685 (2014).","journal-title":"Nature"},{"key":"1338_CR48","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1007\/s11427-015-4864-x","volume":"58","author":"W Hu","year":"2015","unstructured":"Hu, W. & Chen, J. Whole-genome sequencing opens a new era for molecular breeding of grass carp (Ctenopharyngodon idellus). Sci. China Life Sci. \n                           58, 619\u2013620, doi:10.1007\/s11427-015-4864-x (2015).","journal-title":"Sci. China Life Sci."},{"key":"1338_CR49","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1186\/1471-2105-10-421","volume":"10","author":"C Camacho","year":"2009","unstructured":"Camacho, C. et al. BLAST+: architecture and applications. BMC Bioinformatics \n                           10, 421, doi:10.1186\/1471-2105-10-421 (2009).","journal-title":"BMC Bioinformatics"},{"key":"1338_CR50","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1515\/CCLM.2004.004","volume":"42","author":"N Okayama","year":"2004","unstructured":"Okayama, N. et al. Evaluation of a new efficient procedure for single nucleotide polymorphism genotyping: tetra-primer amplification refractory mutation system polymerase chain reaction. Clin. Chem. Lab. Med. \n                           42, 13\u201316, doi:10.1515\/CCLM.2004.004 (2004).","journal-title":"Clin. Chem. Lab. Med."},{"key":"1338_CR51","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1016\/j.fsi.2011.01.006","volume":"30","author":"J Su","year":"2011","unstructured":"Su, J., Zhang, R., Dong, J. & Yang, C. Evaluation of internal control genes for qRT-PCR normalization in tissues and cell culture for antiviral studies of grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. \n                           30, 830\u2013835, doi:10.1016\/j.fsi.2011.01.006 (2011).","journal-title":"Fish Shellfish Immunol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-01338-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-01338-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-01338-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T03:52:21Z","timestamp":1671767541000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-01338-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,26]]},"references-count":51,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["1338"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-01338-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,26]]},"assertion":[{"value":"10 November 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 March 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 April 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"1184"}}