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We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding\n                    <jats:italic>Artemia<\/jats:italic>\n                    . Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercially available fish food using an automated feeding system. We monitored the fasting blood glucose levels in the normal-fed group (one feed\/day) and overfed group (six feeds\/day) over an 8-week period. The fasting blood glucose level was significantly increased in DIO zebrafish compared with that of normal-fed zebrafish. Intraperitoneal and oral glucose tolerance tests showed impaired glucose tolerance by overfeeding. Insulin production, which was determined indirectly by measuring the EGFP signal strength in overfed\n                    <jats:italic>Tg(\u22121.0ins:EGFP)<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>sc1<\/jats:italic>\n                    <\/jats:sup>\n                    zebrafish, was increased in DIO zebrafish. The anti-diabetic drugs metformin and glimepiride ameliorated hyperglycaemia in the overfed group, suggesting that this zebrafish can be used as a model of human T2DM. Finally, we conducted RNA deep sequencing and found that the gene expression profiling of liver-pancreas revealed pathways common to human T2DM. In summary, we developed a zebrafish model of T2DM that shows promise as a platform for mechanistic and therapeutic studies of diet-induced glucose intolerance and insulin resistance.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-01432-w","type":"journal-article","created":{"date-parts":[[2017,4,27]],"date-time":"2017-04-27T08:03:14Z","timestamp":1493280194000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":144,"title":["Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6936-795X","authenticated-orcid":false,"given":"Liqing","family":"Zang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiro","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,5,3]]},"reference":[{"key":"1432_CR1","unstructured":"Federation, I. 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