{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:12:32Z","timestamp":1761581552312,"version":"3.37.3"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Neisseria meningitidis<\/jats:italic> infections are a major issue for global health. The invasive MenC ST-103 clonal complex (CC103) has been the most prevalent in meningococcal outbreaks in Brazil, occurring also in several countries worldwide. Here we have analysed the population structure and accessory genome of MenC CC103 strains from a global perspective. An in-depth phylogenomic analysis revealed a lineage of <jats:italic>N. meningitidis<\/jats:italic> causing meningitis in Brazil and the United Kingdom. This lineage was also characterized as harbouring a particular accessory genome composed of CRISPR\/Cas and restriction modification systems. This lineage was also characterized by a genomic island resembling an integrative and conjugative element. This island carried genes potentially associated with virulence and fitness. We propose this accessory gene repertoire could be contributing to the spatial-temporal persistence of the invasive MenC CC103 lineage.<\/jats:p>","DOI":"10.1038\/s41598-017-01671-x","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T11:04:36Z","timestamp":1493809476000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The invasive Neisseria meningitidis MenC CC103 from Brazil is characterized by an accessory gene repertoire"],"prefix":"10.1038","volume":"7","author":[{"given":"Michel Abanto","family":"Marin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Encinas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhian Almeida","family":"Camargo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roney Santos","family":"Coimbra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0610-8648","authenticated-orcid":false,"given":"Ivano","family":"de Filippis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,5,9]]},"reference":[{"key":"1671_CR1","doi-asserted-by":"publisher","first-page":"566","DOI":"10.3201\/eid1904.111799","volume":"19","author":"OB Harrison","year":"2013","unstructured":"Harrison, O. B. et al. Description and nomenclature of Neisseria meningitidis capsule locus. Emerging infectious diseases \n                           19, 566\u201373, doi:10.3201\/eid1904.111799 (2013).","journal-title":"Emerging infectious diseases"},{"key":"1671_CR2","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/s40121-016-0107-0","volume":"5","author":"N Crum-Cianflone","year":"2016","unstructured":"Crum-Cianflone, N. & Sullivan, E. Meningococcal Vaccinations. Infectious diseases and therapy \n                           5, 89\u2013112, doi:10.1007\/s40121-016-0107-0 (2016).","journal-title":"Infectious diseases and therapy"},{"key":"1671_CR3","doi-asserted-by":"publisher","first-page":"e44102","DOI":"10.1371\/journal.pone.0044102","volume":"7","author":"AB Ibarz-Pav\u00f3n","year":"2012","unstructured":"Ibarz-Pav\u00f3n, A. B. et al. Laboratory-based surveillance of Neisseria meningitidis isolates from disease cases in Latin American and Caribbean countries, SIREVA II 2006\u20132010. PloS one \n                           7, e44102, doi:10.1371\/journal.pone.0044102 (2012).","journal-title":"PloS one"},{"key":"1671_CR4","doi-asserted-by":"publisher","first-page":"e33016","DOI":"10.1371\/journal.pone.0033016","volume":"7","author":"I de Filippis","year":"2012","unstructured":"de Filippis, I. et al. Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. PloS one \n                           7, e33016, doi:10.1371\/journal.pone.0033016 (2012).","journal-title":"PloS one"},{"key":"1671_CR5","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.eimc.2011.07.022","volume":"30","author":"MCO Gorla","year":"2012","unstructured":"Gorla, M. C. O. et al. Phenotypic and molecular characterization of serogroup C Neisseria meningitidis associated with an outbreak in Bahia, Brazil. Enfermedades infecciosas y microbiologia clinica \n                           30, 56\u20139, doi:10.1016\/j.eimc.2011.07.022 (2012).","journal-title":"Enfermedades infecciosas y microbiologia clinica"},{"key":"1671_CR6","doi-asserted-by":"publisher","first-page":"806","DOI":"10.3201\/eid2005.130948","volume":"20","author":"MAP S\u00e1fadi","year":"2014","unstructured":"S\u00e1fadi, M. A. P. et al. Carriage rate and effects of vaccination after outbreaks of serogroup C meningococcal disease, Brazil, 2010. Emerging infectious diseases \n                           20, 806\u201311, doi:10.3201\/eid2005.130948 (2014).","journal-title":"Emerging infectious diseases"},{"key":"1671_CR7","unstructured":"Brasil. Guia de Vigil\u00e2ncia Epidemiol\u00f3gica. Ministerio da Saude (2015)."},{"key":"1671_CR8","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.jinf.2016.05.003","volume":"73","author":"P Stefanelli","year":"2016","unstructured":"Stefanelli, P. et al. Genome-based study of a spatio-temporal cluster of invasive meningococcal disease due to Neisseria meningitidis serogroup C, clonal complex 11. The Journal of infection \n                           73, 136\u201344, doi:10.1016\/j.jinf.2016.05.003 (2016).","journal-title":"The Journal of infection"},{"key":"1671_CR9","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1146\/annurev-genet-120213-092007","volume":"48","author":"E Rotman","year":"2014","unstructured":"Rotman, E. & Seifert, H. S. The genetics of Neisseria species. Annual review of genetics \n                           48, 405\u201331, doi:10.1146\/annurev-genet-120213-092007 (2014).","journal-title":"Annual review of genetics"},{"key":"1671_CR10","doi-asserted-by":"publisher","first-page":"4494","DOI":"10.1073\/pnas.1019751108","volume":"108","author":"S Budroni","year":"2011","unstructured":"Budroni, S. et al. Neisseria meningitidis is structured in clades associated with restriction modification systems that modulate homologous recombination. Proceedings of the National Academy of Sciences of the United States of America \n                           108, 4494\u20139, doi:10.1073\/pnas.1019751108 (2011).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"1671_CR11","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.molcel.2013.05.001","volume":"50","author":"Y Zhang","year":"2013","unstructured":"Zhang, Y. et al. Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis. Molecular cell \n                           50, 488\u2013503, doi:10.1016\/j.molcel.2013.05.001 (2013).","journal-title":"Molecular cell"},{"key":"1671_CR12","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.ebiom.2015.01.004","volume":"2","author":"OB Harrison","year":"2015","unstructured":"Harrison, O. B., Bray, J. E., Maiden, M. C. J. & Caugant, D. A. Genomic Analysis of the Evolution and Global Spread of Hyper-invasive Meningococcal Lineage 5. EBioMedicine \n                           2, 234\u2013243, doi:10.1016\/j.ebiom.2015.01.004 (2015).","journal-title":"EBioMedicine"},{"key":"1671_CR13","doi-asserted-by":"publisher","first-page":"e18441","DOI":"10.1371\/journal.pone.0018441","volume":"6","author":"B Joseph","year":"2011","unstructured":"Joseph, B. et al. Virulence evolution of the human pathogen Neisseria meningitidis by recombination in the core and accessory genome. PloS one \n                           6, e18441, doi:10.1371\/journal.pone.0018441 (2011).","journal-title":"PloS one"},{"key":"1671_CR14","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1046\/j.1365-2958.2001.02520.x","volume":"41","author":"JP Dillard","year":"2001","unstructured":"Dillard, J. P. & Seifert, H. S. A variable genetic island specific for Neisseria gonorrhoeae is involved in providing DNA for natural transformation and is found more often in disseminated infection isolates. Molecular microbiology \n                           41, 263\u201377, doi:10.1046\/j.1365-2958.2001.02520.x (2001).","journal-title":"Molecular microbiology"},{"key":"1671_CR15","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.1111\/j.1365-2958.2005.04521.x","volume":"55","author":"HL Hamilton","year":"2005","unstructured":"Hamilton, H. L., Dom\u00ednguez, N. M., Schwartz, K. J., Hackett, K. T. & Dillard, J. P. Neisseria gonorrhoeae secretes chromosomal DNA via a novel type IV secretion system. 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Infection and immunity \n                           78, 2429\u20132437, doi:10.1128\/IAI.00228-10 (2010).","journal-title":"Infection and immunity"},{"key":"1671_CR18","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.diagmicrobio.2013.04.008","volume":"76","author":"G Sardinha","year":"2013","unstructured":"Sardinha, G. et al. Replacement of Neisseria meningitidis C cc11\/ET-15 variant by a cc103 hypervirulent clone, Brazil 2005\u20132011. Diagnostic microbiology and infectious disease \n                           76, 524\u20135, doi:10.1016\/j.diagmicrobio.2013.04.008 (2013).","journal-title":"Diagnostic microbiology and infectious disease"},{"key":"1671_CR19","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1093\/dnares\/dsi021","volume":"12","author":"M Kawai","year":"2005","unstructured":"Kawai, M., Uchiyama, I. & Kobayashi, I. Genome comparison in silico in Neisseria suggests integration of filamentous bacteriophages by their own transposase. 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