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A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are indirectly calculated through the changes in intensity, band-shape, bandwidth, lifetime or polarization anisotropy of the fluorescence spectra recorded from the nano-sized fluorescent materials pre-injected into the target cell. Unfortunately, the optical properties of the fluorescent nano-materials may be affected by complicated intracellular environment, leading to unexpected measurement errors and controversial arguments. Here, we attempted to offer an alternative approach for measuring the absolute increments of local temperature in micro-Testing Zones induced by live cells. In this method, built-in high-performance micro-thermocouple arrays and double-stabilized system with a stability of 10\u2009mK were applied. Increments of local temperature close to adherent human hepatoblastoma (HepG2) cells were continuously recorded for days without stimulus, showing frequent fluctuations within 60\u2009mK and a maximum increment by 285\u2009mK. This method may open a door for real-time recording of the absolute local temperature increments of individual cells, therefore offering valuable information for cell biology and clinical therapy in the field of cancer research.<\/jats:p>","DOI":"10.1038\/s41598-017-01891-1","type":"journal-article","created":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T15:48:47Z","timestamp":1493912927000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system"],"prefix":"10.1038","volume":"7","author":[{"given":"Fan","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danhong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjie","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,5,11]]},"reference":[{"key":"1891_CR1","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1023\/A:1011993019385","volume":"15","author":"MA Paulik","year":"1998","unstructured":"Paulik, M. A. et al. Development of infrared imaging to measure thermogenesis in cell culture: thermogenic effects of uncoupling protein-2, troglitazone, and beta-adrenoceptor agonists. Pharmaceutical Research. \n                           15, 944\u2013949, doi:10.1023\/A:1011993019385 (1998).","journal-title":"Pharmaceutical Research."},{"key":"1891_CR2","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/S0006-3495(98)77769-0","volume":"74","author":"O Zohar","year":"1998","unstructured":"Zohar, O. et al. Thermal imaging of receptor-activated heat production in single cells. Biophysical Journal. \n                           74, 82\u201389, doi:10.1016\/S0006-3495(98)77769-0 (1998).","journal-title":"Biophysical Journal."},{"key":"1891_CR3","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.copbio.2014.07.013","volume":"31","author":"R Sakaguchi","year":"2015","unstructured":"Sakaguchi, R., Kiyonaka, S. & Mori, Y. 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