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In this experiment, we investigated the function of ANO1 in cardiac fibrosis after myocardial infraction (MI) with methods of Western blotting, Quantitative real-time PCR (qRT-PCR), metabolic reduction of 3-(4,5-dimethylthiozol-2-yl)-2, 5-diphenyltetrazo-lium bromide (MTT), immunofluorescence and confocal imaging, and Masson\u2019s trichrome staining. The results showed that the expression of ANO1 significantly increased in neonatal rats\u2019 cardiac fibroblasts after hypoxia and in cardiac tissues after MI. After ANO1 over-expression, cardiac fibrosis was reduced <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. Moreover, the expression of TGF-\u03b2 and p-smad3 declined after ANO1over-expression in cardiac fiborblasts. In conclusion, ANO1 inhibits cardiac fibrosis after MI via TGF-\u03b2\/smad3 pathway in rats.<\/jats:p>","DOI":"10.1038\/s41598-017-02585-4","type":"journal-article","created":{"date-parts":[[2017,5,18]],"date-time":"2017-05-18T15:13:51Z","timestamp":1495120431000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["ANO1 inhibits cardiac fibrosis after myocardial infraction via TGF-\u03b2\/smad3 pathway"],"prefix":"10.1038","volume":"7","author":[{"given":"Yao","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan Mei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-2065","authenticated-orcid":false,"given":"Li Jun","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,5,24]]},"reference":[{"key":"2585_CR1","unstructured":"Zarrabi, K. et al. 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