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In this study, the ability of the Lab4 probiotic consortium to hydrolyse bile salts, assimilate cholesterol and regulate cholesterol transport by polarised Caco-2 enterocytes was demonstrated. Furthermore, in wild-type C57BL\/6J mice fed a high fat diet, 2-weeks supplementation with Lab4 probiotic consortium plus <jats:italic>Lactobacillus<\/jats:italic><jats:italic>plantarum<\/jats:italic> CUL66 resulted in significant reductions in plasma total cholesterol levels and suppression of diet-induced weight gain. No changes in plasma levels of very low-density lipoprotein\/low-density lipoprotein, high-density lipoprotein, triglycerides, cytokines or bile acids were observed. Increased amounts of total and unconjugated bile acids in the faeces of the probiotic-fed mice, together with modulation of hepatic small heterodimer partner and cholesterol-7\u03b1-hydroxylase mRNA expression, implicates bile salt hydrolase activity as a potential mechanism of action. In summary, this study demonstrates the cholesterol-lowering efficacy of short-term feeding of the Lab4 probiotic consortium plus <jats:italic>L. plantarum<\/jats:italic> CUL66 in wild-type mice and supports further assessment in human trials.<\/jats:p>","DOI":"10.1038\/s41598-017-02889-5","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T11:22:25Z","timestamp":1496229745000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL\/6J mice"],"prefix":"10.1038","volume":"7","author":[{"given":"D. R.","family":"Michael","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. S.","family":"Davies","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. W. 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