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We expressed recombinant salivary proteins from <jats:italic>Lutzomyia intermedi<\/jats:italic>a, a vector of <jats:italic>Leishmania braziliensis<\/jats:italic>, and evaluated the seroreactivity in exposed individuals in search for exposure markers. We found a strong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary proteins: rLinB-13 was the top performing molecule; IgG4 was the most predominant antibody subclass and antibodies to rLinB-13 did not cross react with <jats:italic>Lu. longipalpis<\/jats:italic> salivary proteins. By evaluating a cohort of contacts of CL patients, we confirmed that rLinB-13, an antigen 5-related protein, is a marker of exposure to <jats:italic>Lu. intermedia<\/jats:italic> with high degree of accuracy. In a 5-year follow up, we determined that individuals who developed CL presented higher anti-rLinB13 IgG responses, before the appearance of clinical symptoms. They also presented a lower frequency of cellular responses to the parasite (DTH). Our results show that seroconversion to a salivary molecule, rLinB-13, is a marker of risk for CL development caused by <jats:italic>Leishmania braziliensis<\/jats:italic>. This highlight the possibility of developing tools based on vector molecules to manage the disease in endemic areas.<\/jats:p>","DOI":"10.1038\/s41598-017-03345-0","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T09:40:41Z","timestamp":1496655641000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis"],"prefix":"10.1038","volume":"7","author":[{"given":"Augusto M.","family":"Carvalho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2223-0918","authenticated-orcid":false,"given":"Kiyoshi F.","family":"Fukutani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca P.","family":"Curvelo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Carlos","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldina","family":"Barral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar M.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus G.","family":"Valenzuela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7924-8038","authenticated-orcid":false,"given":"Fabiano","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7868-5164","authenticated-orcid":false,"given":"Camila I.","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,6,9]]},"reference":[{"key":"3345_CR1","doi-asserted-by":"publisher","first-page":"375","DOI":"10.3389\/fimmu.2013.00375","volume":"4","author":"F Oliveira","year":"2013","unstructured":"Oliveira, F., de Carvalho, A. 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