{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:40:25Z","timestamp":1781541625635,"version":"3.54.5"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T00:00:00Z","timestamp":1498435200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T00:00:00Z","timestamp":1498435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tomato wilt pathogen <jats:italic>Fusarium oxysporum<\/jats:italic> f. sp. <jats:italic>lycopersici<\/jats:italic> (<jats:italic>Fol<\/jats:italic>) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of <jats:italic>Fol<\/jats:italic> races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in <jats:italic>secreted in xylem 3<\/jats:italic> (<jats:italic>SIX3<\/jats:italic>) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in <jats:italic>SIX3<\/jats:italic> of <jats:italic>Fol<\/jats:italic>. Loop-mediated isothermal amplification (LAMP) of <jats:italic>SIX3<\/jats:italic> gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect <jats:italic>Fol<\/jats:italic> and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene.<\/jats:p>","DOI":"10.1038\/s41598-017-04084-y","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T13:33:33Z","timestamp":1497965613000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3123-6406","authenticated-orcid":false,"given":"Yu","family":"Ayukawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeri","family":"Hanyuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3323-7314","authenticated-orcid":false,"given":"Ken","family":"Komatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsutomu","family":"Arie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,6,26]]},"reference":[{"key":"4084_CR1","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1111\/j.1364-3703.2009.00605.x","volume":"11","author":"FLW Takken","year":"2010","unstructured":"Takken, F. 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