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Their exact mode of action is an ongoing matter of debate, and several factors have recently been reported to affect an early stage of artemisinin resistance of the most important human malaria parasite <jats:italic>Plasmodium falciparum<\/jats:italic>. Here, we identified a locus on chromosome 7 that affects the artemisinin susceptibility of <jats:italic>P. falciparum<\/jats:italic> in a quantitative trait locus analysis of a genetic cross between strains 7G8 and GB4. This locus includes the peroxiredoxin gene <jats:italic>PFAOP<\/jats:italic>. However, steady-state kinetic data with recombinant <jats:italic>Pf<\/jats:italic>AOP do not support a direct interaction between this peroxidase and the endoperoxide artemisinin. Furthermore, neither the overexpression nor the deletion of the encoding gene affected the IC<jats:sub>50<\/jats:sub> values for artemisinin or the oxidants diamide and <jats:italic>tert<\/jats:italic>-butyl hydroperoxide. Thus, <jats:italic>Pf<\/jats:italic>AOP is dispensable for blood stage parasite survival, and the correlation between the artemisinin susceptibility and chromosome 7 is probably based on another gene within the identified locus.<\/jats:p>","DOI":"10.1038\/s41598-017-04277-5","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T09:12:07Z","timestamp":1498468327000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Knockout of the peroxiredoxin 5 homologue PFAOP does not affect the artemisinin susceptibility of Plasmodium falciparum"],"prefix":"10.1038","volume":"7","author":[{"given":"Carine F.","family":"Djuika","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Staudacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia P.","family":"Sanchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Lanzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Deponte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,6,30]]},"reference":[{"key":"4277_CR1","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1126\/science.3887571","volume":"228","author":"DL Klayman","year":"1985","unstructured":"Klayman, D. L. Qinghaosu (artemisinin): an antimalarial drug from China. Science \n                           228, 1049\u201355 (1985).","journal-title":"Science"},{"key":"4277_CR2","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1038\/nm.2471","volume":"17","author":"Y Tu","year":"2011","unstructured":"Tu, Y. The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine. Nat Med \n                           17, 1217\u201320 (2011).","journal-title":"Nat Med"},{"key":"4277_CR3","unstructured":"W.H.O. Global report on antimalarial efficacy and drug resistance: 2000-2010. http:\/\/www.who.int\/malaria\/publications\/atoz\/9789241500470\/en\/ (2010)."},{"key":"4277_CR4","unstructured":"W.H.O. 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A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Nature \n                           505, 50\u20135 (2014).","journal-title":"Nature"},{"key":"4277_CR8","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1126\/science.1260867","volume":"347","author":"J Straimer","year":"2015","unstructured":"Straimer, J. et al. Drug resistance. K13-propeller mutations confer artemisinin resistance in Plasmodium falciparum clinical isolates. Science \n                           347, 428\u201331 (2015).","journal-title":"Science"},{"key":"4277_CR9","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.mehy.2016.02.022","volume":"90","author":"P Wilairat","year":"2016","unstructured":"Wilairat, P., Kumpornsin, K. & Chookajorn, T. Plasmodium falciparum malaria: Convergent evolutionary trajectories towards delayed clearance following artemisinin treatment. 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D. & Lanzer, M. Genetic predisposition favors the acquisition of stable artemisinin resistance in malaria parasites. Antimicrob Agents Chemother \n                           55, 50\u20135 (2011).","journal-title":"Antimicrob Agents Chemother"},{"key":"4277_CR13","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1586\/eri.09.68","volume":"7","author":"L Cui","year":"2009","unstructured":"Cui, L. & Su, X. Z. Discovery, mechanisms of action and combination therapy of artemisinin. Expert Rev Anti Infect Ther \n                           7, 999\u20131013 (2009).","journal-title":"Expert Rev Anti Infect Ther"},{"key":"4277_CR14","doi-asserted-by":"publisher","first-page":"1705","DOI":"10.3390\/molecules15031705","volume":"15","author":"PM O\u2019Neill","year":"2010","unstructured":"O\u2019Neill, P. M., Barton, V. E. & Ward, S. A. The molecular mechanism of action of artemisinin\u2013the debate continues. 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