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In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations,\n                    <jats:italic>jointless<\/jats:italic>\n                    (\n                    <jats:italic>j<\/jats:italic>\n                    ) and\n                    <jats:italic>jointless-2<\/jats:italic>\n                    (\n                    <jats:italic>j-2<\/jats:italic>\n                    ), controlling pedicel abscission zone formation have been documented but only\n                    <jats:italic>j-2<\/jats:italic>\n                    has been extensively used in breeding.\n                    <jats:italic>J<\/jats:italic>\n                    was shown to encode a MADS-box protein. Using a combination of physical mapping and gene expression analysis we identified a positional candidate,\n                    <jats:italic>Solyc12g038510<\/jats:italic>\n                    , associated with\n                    <jats:italic>j-2<\/jats:italic>\n                    phenotype. Targeted knockout of\n                    <jats:italic>Solyc12g038510<\/jats:italic>\n                    , using CRISPR\/Cas9 system, validated our hypothesis.\n                    <jats:italic>Solyc12g038510<\/jats:italic>\n                    encodes the MADS-box protein SlMBP21. Molecular analysis of\n                    <jats:italic>j-2<\/jats:italic>\n                    natural variation revealed two independent loss-of-function mutants. The first results of an insertion of a\n                    <jats:italic>Rider<\/jats:italic>\n                    retrotransposable element. The second results of a stop codon mutation that leads to a truncated protein form. To bring new insights into the role of\n                    <jats:italic>J<\/jats:italic>\n                    and\n                    <jats:italic>J-2<\/jats:italic>\n                    in abscission zone formation, we phenotyped the single and the double mutants and the engineered alleles. We showed that\n                    <jats:italic>J<\/jats:italic>\n                    is epistatic to\n                    <jats:italic>J-2<\/jats:italic>\n                    and that the branched inflorescences and the leafy sepals observed in accessions harboring\n                    <jats:italic>j-2<\/jats:italic>\n                    alleles are likely the consequences of linkage drags.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-04556-1","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T04:51:45Z","timestamp":1498452705000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":95,"title":["Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato"],"prefix":"10.1038","volume":"7","author":[{"given":"Maria Victoria Gomez","family":"Roldan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7653-2431","authenticated-orcid":false,"given":"Claire","family":"P\u00e9rilleux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halima","family":"Morin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Huerga-Fernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Latrasse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moussa","family":"Benhamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelhafid","family":"Bendahmane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,6,30]]},"reference":[{"key":"4556_CR1","doi-asserted-by":"crossref","unstructured":"van Nocker, S. 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