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This study aimed to introduce\n                    <jats:italic>A. geminatus<\/jats:italic>\n                    into a polymicrobial biofilm model of relevance to periodontitis, and monitor the proteomic responses exerted to the rest of the biofilm community.\n                    <jats:italic>A. geminatus<\/jats:italic>\n                    was grown together with another 10-species in a well-established \u201csubgingival\u201d\n                    <jats:italic>in vitro<\/jats:italic>\n                    biofilm model. Its effects on the other species were quantitatively evaluated by qPCR and label-free proteomics.\n                    <jats:italic>A. geminatus<\/jats:italic>\n                    caused a significant increase in\n                    <jats:italic>P. intermedia<\/jats:italic>\n                    numbers, but not the other species in the biofilm. Whole cell proteome profiling of the biofilms by LC-MS\/MS identified a total of 3213 proteins. Label-free quantitative proteomics revealed that 187 proteins belonging to the other 10 species were differentially abundant when\n                    <jats:italic>A. geminatus<\/jats:italic>\n                    was present in the biofilm. The\u00a0species with most up-regulated and down-regulated proteins were\n                    <jats:italic>P. intermedia<\/jats:italic>\n                    and\n                    <jats:italic>S. oralis<\/jats:italic>\n                    , respectively. Regulated proteins were of primarily of ribosomal origin, and other affected categories involved proteolysis, carbon metabolism and iron transport. In conclusion,\n                    <jats:italic>A. geminatus<\/jats:italic>\n                    can be successfully grown in a polymicrobial biofilm community, causing quantitative proteomic shifts commensurate with increased virulence properties.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-04594-9","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T05:06:51Z","timestamp":1498453611000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Proteomic shifts in multi-species oral biofilms caused by Anaeroglobus geminatus"],"prefix":"10.1038","volume":"7","author":[{"given":"Kai","family":"Bao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagihan","family":"Bostanci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Thurnheer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios N.","family":"Belibasakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,6,30]]},"reference":[{"key":"4594_CR1","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1902\/jop.2011.100638","volume":"82","author":"JM Albandar","year":"2011","unstructured":"Albandar, J. 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