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However, the regulation of their biosynthesis is not fully understood yet. Herein the function of a TetR family regulator GdmRIII, which is located in the biosynthetic gene cluster of geldanamycin, was studied to understand the regulatory mechanism of geldanamycin biosynthesis in <jats:italic>Streptomyces autolyticus<\/jats:italic> CGMCC0516. The production of geldanamycin decreased substantially in a \u0394<jats:italic>gdmRIII<\/jats:italic> mutant and the yield of three compounds which were thought to be geldanamycin congeners greatly increased. Surprisingly, the structural elucidation of these compounds showed that they were elaiophylin and its analogues, which implied that GdmRIII not only played a positive regulatory role in the biosynthesis of geldanamycin, but also played a negative role in elaiophylin biosynthesis. GdmRIII affected the expression of multiple genes in both gene clusters, and directly regulated the expression of <jats:italic>gdmM<\/jats:italic>, <jats:italic>gdmN<\/jats:italic>, and <jats:italic>elaF<\/jats:italic> by binding to the promoter regions of these three genes. A conserved non-palindromic sequence was found among the binding sites of <jats:italic>elaF<\/jats:italic>. Our findings suggested that the biosynthetic pathways of geldanamycin and elaiophylin were connected through GdmRIII, which might provide a way for <jats:italic>Streptomyces<\/jats:italic> to coordinate the biosynthesis of these compounds for better adapting to environment changes.<\/jats:p>","DOI":"10.1038\/s41598-017-05073-x","type":"journal-article","created":{"date-parts":[[2017,6,30]],"date-time":"2017-06-30T11:57:57Z","timestamp":1498823877000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["GdmRIII, a TetR Family Transcriptional Regulator, Controls Geldanamycin and Elaiophylin Biosynthesis in Streptomyces autolyticus CGMCC0516"],"prefix":"10.1038","volume":"7","author":[{"given":"MingXing","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ShaoHua","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiuLin","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KaiYan","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MengLiang","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,6]]},"reference":[{"key":"5073_CR1","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.mib.2005.02.016","volume":"8","author":"MJ Bibb","year":"2005","unstructured":"Bibb, M. 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