{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T22:51:02Z","timestamp":1790549462623,"version":"4.1.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T00:00:00Z","timestamp":1499644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T00:00:00Z","timestamp":1499644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Brain neural stem cells and transit amplifying cells in the subventricular zone (SVZ) of the lateral ventricles are in direct contact with the microvascular endothelium. The mechanisms\/molecules of direct cell contact in the SVZ neurovascular niche are not fully understood. We previously showed that neural stem\/progenitor (NS\/P) cells induce brain endothelial signaling in direct cell contact through matriptase (MTP) on NS\/P cell surface. In the present study, using pull-down and LC-MS\/MS, we identified melanoma cell adhesion molecule (MCAM) the brain endothelial molecule that interacts with MTP. MCAM physically binds to the CUB domains of MTP and induces a chain of brain endothelial signaling including p38MAPK activation, GSK3\u03b2 inactivation and subsequently \u03b2-catenin activation; none of these signaling events occurred when either MTP or MCAM is deleted. MTP-MCAM binding and induction of endothelial signaling were all sensitive to cholera toxin. Together, we identified key molecules that may represent a mechanism in neural stem cell vascular niche regulation.<\/jats:p>","DOI":"10.1038\/s41598-017-05131-4","type":"journal-article","created":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T09:23:33Z","timestamp":1499160213000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase\u2014Novel Cell Adhesion in Neural Stem cell Vascular Niche"],"prefix":"10.1038","volume":"7","author":[{"given":"Hsiu-Hui","family":"Tung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheau-Ling","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,10]]},"reference":[{"key":"5131_CR1","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/j.stem.2008.07.026","volume":"3","author":"Q Shen","year":"2008","unstructured":"Shen, Q. et al. Adult SVZ Stem Cells Lie in a Vascular Niche: A Quantitative Analysis of Niche Cell-Cell Interactions. Cell Stem Cell \n                           3, 289\u2013300 (2008).","journal-title":"Cell Stem Cell"},{"key":"5131_CR2","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.stem.2008.07.025","volume":"3","author":"M Tavazoie","year":"2008","unstructured":"Tavazoie, M. et al. A Specialized Vascular Niche for Adult Neural Stem Cells. Cell Stem Cell \n                           3, 279\u2013288 (2008).","journal-title":"Cell Stem Cell"},{"key":"5131_CR3","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.stem.2008.07.004","volume":"3","author":"Z Mirzadeh","year":"2008","unstructured":"Mirzadeh, Z., Merkle, F. T., Soriano-Navarro, M., Garcia-Verdugo, J. M. & Alvarez-Buylla, A. Neural Stem Cells Confer Unique Pinwheel Architecture to the Ventricular Surface in Neurogenic Regions of the Adult Brain. 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