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The tegument that surrounds the worm is critical to the schistosomes survival. The tegument apical membrane undergoes a continuous process of rupture and repair owing to membranous vacuoles fusing with the plasma membrane.<jats:underline>V<\/jats:underline>esicle-<jats:underline>a<\/jats:underline>ssociated<jats:underline>m<\/jats:underline>embrane<jats:underline>p<\/jats:underline>rotein<jats:underline>2<\/jats:underline>(VAMP2), a member of<jats:underline>s<\/jats:underline>oluble<jats:underline>N<\/jats:underline>-ethylmaleimide sensitive factor<jats:underline>a<\/jats:underline>ttachment protein<jats:underline>re<\/jats:underline>ceptor (SNAREs) is required for membrane fusion. Here, we used RNA interference (RNAi) to knock down the expression of VAMP2 of<jats:italic>Schistosoma japonicum<\/jats:italic>(<jats:italic>SjVAMP2<\/jats:italic>), and both real-time PCR and western blot analysis confirmed the suppression of this molecule, as well as the suppression of the transcript levels of schistosome glucose transporters (<jats:italic>SGTP1<\/jats:italic>and<jats:italic>SGTP4<\/jats:italic>), and insulin receptors (<jats:italic>SjIR1<\/jats:italic>and<jats:italic>SjIR2<\/jats:italic>).<jats:italic>SjVAMP2<\/jats:italic>-suppressed worms exhibited a lower viability, and phenotypic alterations were also observed in the tegument. Moreover, the glucose consumption of<jats:italic>SjVAMP2<\/jats:italic>-suppressed worms decreased significantly in 4 and 6 days, respectively, as well as a significant reduction in egg production. We also observed a significant reduction in worm burden and hepatic eggs burden in two independent RNAi experiment<jats:italic>in vivo<\/jats:italic>, and minor pathological changes in mice treated with<jats:italic>SjVAMP2<\/jats:italic>specific small interfering (si)RNA. These findings reveal that<jats:italic>SjVAMP2<\/jats:italic>may play important roles in the maintenance of tegument, glucose uptake, worm development and egg production in schistosomes.<\/jats:p>","DOI":"10.1038\/s41598-017-05602-8","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T10:12:15Z","timestamp":1499335935000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum"],"prefix":"10.1038","volume":"7","author":[{"given":"Qian","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingguang","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuangang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,12]]},"reference":[{"key":"5602_CR1","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.1056\/NEJMra012396","volume":"346","author":"AG Ross","year":"2002","unstructured":"Ross, A. 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