{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:04:08Z","timestamp":1791281048118,"version":"4.1.0"},"reference-count":70,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,7,14]],"date-time":"2017-07-14T00:00:00Z","timestamp":1499990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,7,14]],"date-time":"2017-07-14T00:00:00Z","timestamp":1499990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ageing is a broad cellular process, largely affecting the immune system, especially T-lymphocytes. Additionally to immunosenescence alone, cytomegalovirus (CMV) infection is thought to have major impacts on T-cell subset composition and exhaustion. These impacts have been studied extensively in TCR\u03b1\u03b2+ T-cells, with reduction in naive, increase in effector (memory) subsets and shifts in CD4\/CD8-ratios, in conjunction with morbidity and mortality in elderly. Effects of both ageing and CMV on the TCR\u03b3\u03b4+ T-cell compartment remain largely elusive. In the current study we investigated V\u03b3- and V\u03b4-usage, maturation, differentiation and exhaustion marker profiles of both CD4 and CD8 double-negative (DN) and CD8+TCR\u03b3\u03b4+ T-cells in 157 individuals, age range 20\u201395. We observed a progressive decrease in absolute numbers of total TCR\u03b3\u03b4+ T-cells in blood, affecting the predominant V\u03b39\/V\u03b42 population. Aged TCR\u03b3\u03b4+ T-cells appeared to shift from naive to more (late-stage) effector phenotypes, which appeared more prominent in case of persistent CMV infections. In addition, we found effects of both ageing and CMV on the absolute counts of exhausted TCR\u03b3\u03b4+ T-cells. Collectively, our data show a clear impact of ageing and CMV persistence on DN and CD8+TCR\u03b3\u03b4+ T-cells, similar to what has been reported in CD8+TCR\u03b1\u03b2+ T-cells, indicating that they undergo similar ageing processes.<\/jats:p>","DOI":"10.1038\/s41598-017-05849-1","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T15:16:23Z","timestamp":1499699783000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells"],"prefix":"10.1038","volume":"7","author":[{"given":"Martine J.","family":"Kallemeijn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Mieke H.","family":"Boots","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le Y.","family":"van der Klift","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Brouwer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayel H.","family":"Abdulahad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A. N.","family":"Verhaar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J. M.","family":"van Dongen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton W.","family":"Langerak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,14]]},"reference":[{"key":"5849_CR1","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1007\/978-94-007-2869-1_14","volume":"942","author":"HC Lee","year":"2012","unstructured":"Lee, H. C. & Wei, Y. H. Mitochondria and ageing. Adv. Exp. Med. Biol. \n                           942, 311\u2013327 (2012).","journal-title":"Adv. Exp. Med. Biol."},{"key":"5849_CR2","unstructured":"Gilbert, S. F. 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