{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:39:12Z","timestamp":1790055552453,"version":"4.0.1"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T00:00:00Z","timestamp":1502841600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T00:00:00Z","timestamp":1502841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diverticular disease is commonly associated with the older population in the United States. As individual\u2019s age, diverticulae, or herniation of the mucosa through the colonic wall, develop. In 10\u201325% of individuals, the diverticulae become inflamed, resulting in diverticulitis. The gut ecosystem relies on the interaction of bacteria and fungi to maintain homeostasis. Although bacterial dysbiosis has been implicated in the pathogenesis of diverticulitis, associations between the microbial ecosystem and diverticulitis remain largely unstudied. This study investigated how the cooperative network of bacteria and fungi differ between a diseased area of the sigmoid colon chronically affected by diverticulitis and adjacent non-affected tissue. To identify mucosa-associated microbes, bacterial 16S rRNA and fungal ITS sequencing were performed on chronically diseased sigmoid colon tissue (DT) and adjacent tissue (AT) from the same colonic segment. We found that <jats:italic>Pseudomonas<\/jats:italic> and <jats:italic>Basidiomycota<\/jats:italic> OTUs were associated with AT while <jats:italic>Microbacteriaceae<\/jats:italic> and <jats:italic>Ascomycota<\/jats:italic> were enriched in DT. Bipartite co-occurrence networks were constructed for each tissue type. The DT and AT networks were distinct for each tissue type, with no microbial relationships maintained after intersection merge of the groups. Our findings indicate that the microbial ecosystem distinguishes chronically diseased tissue from adjacent tissue.<\/jats:p>","DOI":"10.1038\/s41598-017-06787-8","type":"journal-article","created":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T15:06:00Z","timestamp":1502377560000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["The Microbial Ecosystem Distinguishes Chronically Diseased Tissue from Adjacent Tissue in the Sigmoid Colon of Chronic, Recurrent Diverticulitis Patients"],"prefix":"10.1038","volume":"7","author":[{"given":"Kathleen M.","family":"Schieffer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Sabey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin R.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Toole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3919-6525","authenticated-orcid":false,"given":"Rebecca","family":"Drucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasily","family":"Tokarev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard R.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Deiling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3814-5286","authenticated-orcid":false,"given":"Melanie A.","family":"Eshelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Hegarty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory S.","family":"Yochum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter A.","family":"Koltun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Lamendella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Sr.","given":"David B.","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,16]]},"reference":[{"key":"6787_CR1","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1128\/MMBR.00020-11","volume":"75","author":"P Frey-Klett","year":"2011","unstructured":"Frey-Klett, P. et al. Bacterial-fungal interactions: hyphens between agricultural, clinical, environmental, and food microbiologists. Microbiol. Mol. Biol. Rev. \n                           75, 583\u2013609 (2011).","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"6787_CR2","doi-asserted-by":"publisher","first-page":"6ra14","DOI":"10.1126\/scitranslmed.3000322","volume":"1","author":"P Turnbaugh","year":"2009","unstructured":"Turnbaugh, P. et al. The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice. Sci. Transl. Med. \n                           1, 6ra14 (2009).","journal-title":"Sci. Transl. Med."},{"key":"6787_CR3","doi-asserted-by":"publisher","first-page":"R79","DOI":"10.1186\/gb-2012-13-9-r79","volume":"13","author":"XC Morgan","year":"2012","unstructured":"Morgan, X. C. et al. Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment. 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A fungal signature in the gut microbiota of pediatric patients with inflammatory bowel disease. Inflamm. Bowel Dis. \n                           21, 1948\u20131956 (2015).","journal-title":"Inflamm. Bowel Dis."},{"key":"6787_CR7","first-page":"21","volume":"9","author":"VB Reddy","year":"2013","unstructured":"Reddy, V. B. & Longo, W. E. The burden of diverticular disease on patients and healthcare systems. Gastroenterol. Hepatol. (N Y) \n                           9, 21\u201327 (2013).","journal-title":"Gastroenterol. Hepatol. (N Y)"},{"key":"6787_CR8","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1055\/s-2004-832695","volume":"17","author":"KG Hobson","year":"2004","unstructured":"Hobson, K. G. & Roberts, P. L. Etiology and pathophysiology of diverticular disease. Clin. Colon Rectal Surg. \n                           17, 147\u2013153 (2004).","journal-title":"Clin. Colon Rectal Surg."},{"key":"6787_CR9","unstructured":"Thorson, A. & Beaty, J. 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