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However, their impact on the gut\u2019s bacterial microbiota remains uncertain. We characterised the intestinal microbiota in 6\u201311 month-old infants in India who received a 3-day course of azithromycin or placebo during a randomised trial of oral poliovirus vaccine immunogenicity (CTRI\/2014\/05\/004588). In 60 infants per study arm, we sequenced the V4 region of the bacterial 16S rRNA gene in stool samples collected before and 12 days after finishing treatment. We also tested for the presence of common bacterial, viral, and eukaryotic enteropathogens in the same samples using real-time PCR in a Taqman array card (TAC) format. Azithromycin induced a modest decline in microbiota richness and a shift in taxonomic composition driven by a reduction in the relative abundance of Proteobacteria and Verrucomicrobia (specifically<jats:italic>Akkermansia muciniphila<\/jats:italic>). The former phylum includes pathogenic strains of<jats:italic>Escherichia coli<\/jats:italic>and<jats:italic>Campylobacter<\/jats:italic>spp. that declined in prevalence based on the TAC assay. These findings differ from previous observations among older children and adults in Europe and North America, suggesting that the effects of azithromycin on the bacterial microbiota may be specific to the age and geographic setting of its recipients.<\/jats:p>","DOI":"10.1038\/s41598-017-06862-0","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T07:51:43Z","timestamp":1502956303000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Changes in the intestinal microbiota following the administration of azithromycin in a randomised placebo-controlled trial among infants in south India"],"prefix":"10.1038","volume":"7","author":[{"given":"Edward P. 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