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Fifth-passage renal interstitial fibroblasts (RIFs) from the three groups were analyzed for senescence and proliferation. The effect of <jats:italic>Bmi<\/jats:italic>-<jats:italic>1<\/jats:italic> deficiency on epithelial-to-mesenchymal transition (EMT) was examined in <jats:italic>Bmi<\/jats:italic>-<jats:italic>1<\/jats:italic>-knockdown human renal proximal tubular epithelial (HK2) cells, which were treated with concentrated conditioned medium (CM) from the fifth-passage renal interstitial fibroblasts (RIFs) of above three group mice or with exogenous TGF-\u03b21. Our results demonstrated that <jats:italic>p16<\/jats:italic>\n            <jats:sup>\n              <jats:italic>INK4a<\/jats:italic>\n            <\/jats:sup> deletion largely rescued renal aging phenotypes caused by <jats:italic>Bmi<\/jats:italic>-<jats:italic>1<\/jats:italic> deficiency, including impaired renal structure and function, decreased proliferation, increased apoptosis, senescence and SASP, DNA damage, NF-\u03baB and TGF-\u03b21\/Smad signal activation, inflammatory cell infiltration, and tubulointerstitial fibrosis and tubular atrophy. <jats:italic>P16<\/jats:italic>\n            <jats:sup>\n              <jats:italic>INK4a<\/jats:italic>\n            <\/jats:sup> deletion also promoted proliferation, reduced senescence and SASP of RIFs and subsequently inhibited EMT of <jats:italic>Bmi<\/jats:italic>-<jats:italic>1<\/jats:italic>-knockdown HK2 cells. TGF-\u03b21 further induced the EMT of <jats:italic>Bmi<\/jats:italic>-<jats:italic>1<\/jats:italic>-knockdown HK2 cells. Thus, <jats:italic>p16<\/jats:italic>\n            <jats:sup>\n              <jats:italic>INK4a<\/jats:italic>\n            <\/jats:sup> positive senescent cells would be a therapeutic target for preventing renal tubulointerstitial injury.<\/jats:p>","DOI":"10.1038\/s41598-017-06868-8","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T08:13:33Z","timestamp":1501661613000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["RETRACTED ARTICLE: P16INK4a Deletion Ameliorated Renal Tubulointerstitial Injury in a Stress-induced Premature Senescence Model of Bmi-1 Deficiency"],"prefix":"10.1038","volume":"7","author":[{"given":"Jianliang","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhui","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengshun","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,8]]},"reference":[{"key":"6868_CR1","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1007\/s00467-011-1992-9","volume":"27","author":"KS Hodgkins","year":"2012","unstructured":"Hodgkins, K. 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