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We investigated the ability of <jats:italic>P. acnes<\/jats:italic> surface proteins to recognize ECM proteins and showed that a 58\u2009kDa\u2009<jats:italic>P. acnes<\/jats:italic> surface protein was specifically recognized by human fibrinogen (hFg). The 58\u2009kDa protein was further characterized by two-dimensional (2-D) electrophoresis and MALDI-ToF as a <jats:italic>P. acnes<\/jats:italic> host cell-surface attachment protein, PA25957, recognizing dermatan sulfate (DsA1). This protein sequence contains 432 amino acids with the presence of three structurally different domains: an N-terminal signal peptide, a C-terminal LPXTG motif, and a PT repeat region. DsA1 is mostly produced during stationary phase. It appears to be highly glycosylated, containing GalNAc residues. Purified DsA1 strongly recognizes the A\u03b1 and B\u03b2 subunits of hFg, and specific enzymatic deglycosylation of hFg demonstrated the involvement of the protein backbone in the recognition process. The B\u03b2 subunit of hFg was cloned in four peptide fractions (Fg1-Fg4). The N-terminal Fg1 peptide of hFg was recognized by DsA1, and priming DsA1 with Fg1 inhibited DsA1\/hFg recognition. We describe here for the first time, the characterization of a <jats:italic>P. acnes<\/jats:italic> surface glycoprotein recognizing human fibrinogen.<\/jats:p>","DOI":"10.1038\/s41598-017-06940-3","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T13:55:20Z","timestamp":1500472520000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Characterization of a Propionibacterium acnes Surface Protein as a Fibrinogen-Binding Protein"],"prefix":"10.1038","volume":"7","author":[{"given":"Philippe A.","family":"Grange","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00ebl","family":"Raingeaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willy","family":"Morelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Genevi\u00e8ve","family":"Marcelin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Calvez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Dupin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,25]]},"reference":[{"key":"6940_CR1","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1093\/oxfordjournals.jbchem.a133918","volume":"92","author":"K Kamisango","year":"1982","unstructured":"Kamisango, K. et al. Structures and biological activities of peptidoglycans of Listeria monocytogenes and Propionibacterium acnes. J Biochem \n                           92, 23\u201333 (1982).","journal-title":"J Biochem"},{"key":"6940_CR2","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1128\/CMR.00092-13","volume":"27","author":"Y Achermann","year":"2014","unstructured":"Achermann, Y., Goldstein, E. J. C., Coenye, T. & Shirtliff, M. E. Propionibacterium acnes: from commensal to opportunistic biofilm-associated implant pathogen. Clin Microbiol Rev \n                           27, 419\u2013440 (2014).","journal-title":"Clin Microbiol Rev"},{"key":"6940_CR3","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.clindermatol.2009.03.012","volume":"28","author":"C Dessinioti","year":"2010","unstructured":"Dessinioti, C. & Katsambas, A. D. The role of Propionibacterium acnes in acne pathogenesis: facts and controversies. 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Propionibacterium acnes endocarditis: a case series. Clin Microbiol Infect \n                           2, S1198-743X(16)30658-9; doi:10.1016\/j.cmi.2016.12.026 (2017).","DOI":"10.1016\/j.cmi.2016.12.026"},{"key":"6940_CR9","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1128\/JCM.40.1.198-204.2002","volume":"40","author":"Y Eishi","year":"2002","unstructured":"Eishi, Y. et al. Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis. J Clin Microbiol \n                           40, 198\u2013204 (2002).","journal-title":"J Clin Microbiol"},{"key":"6940_CR10","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.ijmm.2016.06.006","volume":"306","author":"B Sayanlali","year":"2016","unstructured":"Sayanlali, B. et al. Propionibacterium acnes inhibits FOXM1 and induces cell cycle alterations in human primary prostate cells. 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