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Recently, supplements or warming devices to relieve presbyopic symptoms have been developed, but no eye drops have been developed. We screened certain compounds possibly related to lens degeneration and identified pirenoxine, which has been used for cataracts, as a possible new pharmacologic treatment for presbyopia. We first researched the anti-presbyopic activity of pirenoxine in rats. The lens elasticity significantly (<jats:italic>p<\/jats:italic>\u2009=\u20090.028) increased with exposure to tobacco smoke for 12 days, and pirenoxine eye drops significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) suppressed lens hardening, which causes presbyopia in humans. In a parallel randomized controlled clinical study of the subjects in their fifth decade of life, the objective accommodative amplitude (AA) decreased significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) by 0.16 diopter (D) in the control group, and there was no detectable change in the treatment group after a 6-month treatment period, suggesting that pirenoxine eye drops might prevent progression of presbyopia. Subjects in their sixth decade of life, in whom the AA was already nearly 0 D, did not show similar results. Pirenoxine eye drops might be a new and the first pharmacologic treatment for preventing progression of presbyopia.<\/jats:p>","DOI":"10.1038\/s41598-017-07208-6","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T13:20:21Z","timestamp":1500902421000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Suppression of presbyopia progression with pirenoxine eye drops: experiments on rats and non-blinded, randomized clinical trial of efficacy"],"prefix":"10.1038","volume":"7","author":[{"given":"Yukari","family":"Tsuneyoshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Higuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuno","family":"Negishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8874-7111","authenticated-orcid":false,"given":"Kazuo","family":"Tsubota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,7,28]]},"reference":[{"key":"7208_CR1","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1146\/annurev-bioeng-071813-105216","volume":"16","author":"NJ Durr","year":"2014","unstructured":"Durr, N. 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Kazuo Tsubota has licensed it to Tsubota Laboratory, Inc. Kazuo Tsubota has the patent for the method and apparatus for measuring the operating VA and receives royalties from Kowa Ltd.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"6819"}}