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The mechanisms responsible for this ATP release are poorly understood. CALHM1, a transmembrane protein with shared structural features to connexins and pannexins, has been implicated in ATP release from taste buds, but it has not been evaluated for a functional role in the airway. In the present study,<jats:italic>Calhm1<\/jats:italic>knockout,<jats:italic>Panx1<\/jats:italic>knockout, and wild-type mouse nasal septal epithelial cells were grown at an air-liquid interface (ALI) and subjected to light mechanical stimulation from an air puff. Apical ATP release was attenuated in<jats:italic>Calhm1<\/jats:italic>knockout cultures following mechanical stimulation at a pressure of 55\u2009mmHg for 50 milliseconds (p\u2009&lt;\u20090.05). Addition of carbenoxolone, a PANX1 channel blocker, completely abolished ATP release in<jats:italic>Calhm1<\/jats:italic>knockout cultures but not in wild type or<jats:italic>Panx1<\/jats:italic>knockout cultures. An increase in CBF was observed in wild-type ALIs following mechanical stimulation, and this increase was significantly lower (p\u2009&lt;\u20090.01) in<jats:italic>Calhm1<\/jats:italic>knockout cultures. These results demonstrate that CALHM1 plays a newly defined role, complementary to PANX1, in ATP release and downstream CBF modulation following a mechanical stimulus in airway epithelial cells.<\/jats:p>","DOI":"10.1038\/s41598-017-07221-9","type":"journal-article","created":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T10:48:44Z","timestamp":1500634124000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["CALHM1-Mediated ATP Release and Ciliary Beat Frequency Modulation in Nasal Epithelial Cells"],"prefix":"10.1038","volume":"7","author":[{"given":"Alan D.","family":"Workman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan M.","family":"Carey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecil J.","family":"Saunders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Marambaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire H.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Tordoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noam A.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,27]]},"reference":[{"key":"7221_CR1","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1007\/s00424-002-0955-1","volume":"445","author":"RC Boucher","year":"2003","unstructured":"Boucher, R. 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