{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:02:59Z","timestamp":1758628979472},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T00:00:00Z","timestamp":1502150400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T00:00:00Z","timestamp":1502150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conventional cytopathology examination of respiratory samples can aid in identifying <jats:italic>Aspergillus<\/jats:italic> but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of <jats:italic>Aspergillus<\/jats:italic> in respiratory samples following Papanicolaou\u2019s or Special staining with Grocott\u2019s methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n\u2009=\u200954) and sputum samples (n\u2009=\u2009117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT. LCT slides were generally superior to conventional slides, showing smaller cell monolayer surface area, clearer background and more distinct stereoscopic cytological features. For Papanicolaou\u2019s staining, LCT-prepared slides allowed a higher positive rate of <jats:italic>Aspergillus<\/jats:italic> identification than conventional slides for bronchial brushing samples (59.25% vs. 20.37%, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and sputum samples (29.05% vs. 8.55%, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Similarly, Special staining of LCT-prepared slides showed a higher positive rate of <jats:italic>Aspergillus<\/jats:italic> identification for bronchial brushing samples (83.33% vs. 57.41%, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and sputum samples (43.59% vs. 19.66%, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). This preliminary study suggests that LCT may be better than conventional slide preparation for identifying <jats:italic>Aspergillus<\/jats:italic> in respiratory samples from patients with pulmonary aspergillosis.<\/jats:p>","DOI":"10.1038\/s41598-017-07564-3","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T08:46:29Z","timestamp":1501663589000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis"],"prefix":"10.1038","volume":"7","author":[{"given":"Yongchun","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaorong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyi","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,8,8]]},"reference":[{"key":"7564_CR1","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1183\/09059180.00001011","volume":"20","author":"M Kousha","year":"2011","unstructured":"Kousha, M., Tadi, R. & Soubani, A. O. Pulmonary aspergillosis: a clinical review. Eur Respir Rev \n                           20, 156\u201374 (2011).","journal-title":"Eur Respir Rev"},{"key":"7564_CR2","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1136\/thoraxjnl-2014-206291","volume":"70","author":"C Kosmidis","year":"2015","unstructured":"Kosmidis, C. & Denning, D. W. The clinical spectrum of pulmonary aspergillosis. Thorax \n                           70, 270\u2013277 (2015).","journal-title":"Thorax"},{"key":"7564_CR3","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.idc.2015.10.015","volume":"30","author":"J Cadena","year":"2016","unstructured":"Cadena, J., Thompson, G. R. 3rd & Patterson, T. F. Invasive Aspergillosis: Current Strategies for Diagnosis and Management. Infect Dis Clin North Am \n                           30, 125\u2013142 (2016).","journal-title":"Infect Dis Clin North Am"},{"key":"7564_CR4","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1086\/592255","volume":"47","author":"Y Nivoix","year":"2008","unstructured":"Nivoix, Y. et al. Factors associated with overall and attributable mortality in invasive aspergillosis. Clin Infect Dis \n                           47, 1176\u20131184 (2008).","journal-title":"Clin Infect Dis"},{"key":"7564_CR5","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1159\/000196477","volume":"62","author":"M von Eiff","year":"1995","unstructured":"von Eiff, M. et al. Pulmonary aspergillosis: early diagnosis improves survival. Respiration \n                           62, 341\u2013347 (1995).","journal-title":"Respiration"},{"key":"7564_CR6","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1007\/s11046-014-9763-3","volume":"178","author":"J Guinea","year":"2014","unstructured":"Guinea, J. & Bouza, E. Current challenges in the microbiological diagnosis of invasive aspergillosis. Mycopathologia \n                           178, 403\u2013416 (2014).","journal-title":"Mycopathologia"},{"key":"7564_CR7","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.medmal.2013.11.006","volume":"44","author":"G Desoubeaux","year":"2014","unstructured":"Desoubeaux, G., Bailly, \u00c9. & Chandenier, J. Diagnosis of invasive pulmonary aspergillosis: updates and recommendations. Med Mal Infect \n                           44, 89\u2013101 (2014).","journal-title":"Med Mal Infect"},{"key":"7564_CR8","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/S1473-3099(15)70006-X","volume":"15","author":"S Schelenz","year":"2015","unstructured":"Schelenz, S. et al. British Society for Medical Mycology best practice recommendations for the diagnosis of serious fungal diseases. Lancet Infect Dis \n                           15, 461\u2013474 (2015).","journal-title":"Lancet Infect Dis"},{"key":"7564_CR9","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1002\/dc.22842","volume":"41","author":"RS Hoda","year":"2013","unstructured":"Hoda, R. S., Loukeris, K. & Abdul-Karim, F. W. Gynecologic cytology on conventional and liquid-based preparations: a comprehensive review of similarities and differences. Diagn Cytopathol \n                           41, 257\u2013278 (2013).","journal-title":"Diagn Cytopathol"},{"key":"7564_CR10","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1007\/s12020-015-0835-z","volume":"53","author":"SY Kim","year":"2016","unstructured":"Kim, S. Y. et al. Combined use of conventional smear and liquid-based preparation versus conventional smear for thyroid fine-needle aspiration. Endocrine \n                           53, 157\u2013165 (2016).","journal-title":"Endocrine"},{"key":"7564_CR11","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1159\/000369198","volume":"58","author":"CW Michael","year":"2014","unstructured":"Michael, C. W. & Bedrossian, C. C. The implementation of liquid-based cytology for lung and pleural-based diseases. Acta Cytol \n                           58, 563\u2013573 (2014).","journal-title":"Acta Cytol"},{"key":"7564_CR12","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1159\/000362805","volume":"58","author":"R Gerhard","year":"2014","unstructured":"Gerhard, R. & Schmitt, F. C. Liquid-based cytology in fine-needle aspiration of breast lesions: a review. Acta Cytol \n                           58, 533\u2013542 (2014).","journal-title":"Acta Cytol"},{"key":"7564_CR13","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1586\/14737140.2015.1053874","volume":"15","author":"ED Rossi","year":"2015","unstructured":"Rossi, E. D., Bizzarro, T., Longatto-Filho, A., Gerhard, R. & Schmitt, F. The diagnostic and prognostic role of liquid-based cytology: are we ready to monitor therapy and resistance? Expert Rev Anticancer Ther \n                           15, 911\u201321 (2015).","journal-title":"Expert Rev Anticancer Ther"},{"key":"7564_CR14","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1159\/000366260","volume":"58","author":"B Abedi-Ardekani","year":"2014","unstructured":"Abedi-Ardekani, B. & Vielh, P. Is liquid-based cytology the magic bullet for performing molecular techniques? Acta Cytol \n                           58, 574\u2013581 (2014).","journal-title":"Acta Cytol"},{"key":"7564_CR15","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1111\/j.1440-1843.2008.01399.x","volume":"14","author":"GP Wu","year":"2009","unstructured":"Wu, G. P., Wang, E. H., Li, J. H., Fu, Z. M. & Han, S. Clinical application of the liquid-based cytological test in cytological screening of sputum for the diagnosis of lung cancer. Respirology \n                           14, 124\u2013128 (2009).","journal-title":"Respirology"},{"key":"7564_CR16","doi-asserted-by":"publisher","first-page":"1813","DOI":"10.1086\/588660","volume":"46","author":"B De Pauw","year":"2008","unstructured":"De Pauw, B. et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG) Consensus Group. Clin Infect Dis \n                           46, 1813\u20131821 (2008).","journal-title":"Clin Infect Dis"},{"key":"7564_CR17","first-page":"821","volume":"30","author":"Infectious disease Committee of Chinese Thoracic Society","year":"2007","unstructured":"Infectious disease Committee of Chinese Thoracic Society. Expert consensus on the diagnosis and treatment of pulmonary fungus diseases. Zhonghua Jie He He Hu Xi Za Zhi \n                           30, 821\u2013834 (2007).","journal-title":"Zhonghua Jie He He Hu Xi Za Zhi"},{"key":"7564_CR18","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1183\/13993003.00583-2015","volume":"47","author":"DW Denning","year":"2016","unstructured":"Denning, D. W. et al. Chronic pulmonary aspergillosis: rationale and clinical guidelines for diagnosis and management. Eur Respir J \n                           47, 45\u201368 (2016).","journal-title":"Eur Respir J"},{"key":"7564_CR19","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s10552-015-0678-1","volume":"27","author":"K Rozemeijer","year":"2016","unstructured":"Rozemeijer, K. et al. Comparing SurePath, ThinPrep, and conventional cytology as primary test method: SurePath is associated with increased CIN II+ detection rates. Cancer Causes Control \n                           27, 15\u201325 (2016).","journal-title":"Cancer Causes Control"},{"key":"7564_CR20","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1159\/000376545","volume":"59","author":"T Qiu","year":"2015","unstructured":"Qiu, T. et al. Liquid-Based Cytology Preparation Can Improve Cytological Assessment of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration. Acta Cytol \n                           59, 139\u2013143 (2015).","journal-title":"Acta Cytol"},{"key":"7564_CR21","first-page":"242","volume":"16","author":"R Sherif","year":"2010","unstructured":"Sherif, R. & Segal, B. H. Pulmonary aspergillosis: clinical presentation, diagnostic tests, management and complications. Curr Opin Pulm Med \n                           16, 242\u2013250 (2010).","journal-title":"Curr Opin Pulm Med"},{"key":"7564_CR22","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1159\/000196477","volume":"62","author":"M Von. Eiff.","year":"1995","unstructured":"von Eiff, M. et al. Pulmonary aspergillosis: early diagnosis improves survival. Respiration \n                           62, 341\u2013347 (1995).","journal-title":"Respiration"},{"key":"7564_CR23","doi-asserted-by":"publisher","first-page":"1094","DOI":"10.1016\/j.rmed.2013.04.007","volume":"107","author":"Y Kono","year":"2013","unstructured":"Kono, Y. et al. The utility of galactomannan antigen in the bronchial washing and serum for diagnosing pulmonary aspergillosis. Respir Med \n                           107, 1094\u20131100 (2013).","journal-title":"Respir Med"},{"key":"7564_CR24","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1086\/503427","volume":"42","author":"CD Pfeiffer","year":"2006","unstructured":"Pfeiffer, C. D., Fine, J. P. & Safdar, N. Diagnosis of invasive aspergillosis using a galactomannan assay: a meta-analysis. Clin Infect Dis \n                           42, 1417\u20131427 (2006).","journal-title":"Clin Infect Dis"},{"key":"7564_CR25","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1128\/JCM.05267-11","volume":"50","author":"A Onishi","year":"2012","unstructured":"Onishi, A. et al. Diagnostic accuracy of serum 1,3-\u03b2-D-glucan for pneumocystis jiroveci pneumonia, invasive candidiasis, and invasive aspergillosis: systematic review and meta-analysis. J Clin Microbiol \n                           50, 7\u201315 (2012).","journal-title":"J Clin Microbiol"},{"key":"7564_CR26","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.1128\/JCM.01365-14","volume":"52","author":"M Arvanitis","year":"2014","unstructured":"Arvanitis, M. et al. PCR in diagnosis of invasive aspergillosis: a meta-analysis of diagnostic performance. J Clin Microbiol \n                           52, 3731\u20133742 (2014).","journal-title":"J Clin Microbiol"},{"key":"7564_CR27","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1136\/thx.44.10.822","volume":"44","author":"H Verea-Hernando","year":"1989","unstructured":"Verea-Hernando, H., Martin-Ega\u00f1a, M. T., Montero-Martinez, C. & Fontan-Bueso, J. Bronchoscopy findings in invasive pulmonary aspergillosis. Thorax \n                           44, 822\u2013823 (1989).","journal-title":"Thorax"},{"key":"7564_CR28","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.micinf.2006.10.009","volume":"9","author":"C Com\u00e9ra","year":"2007","unstructured":"Com\u00e9ra, C. et al. Gliotoxin from Aspergillus fumigatus affects phagocytosis and the organization of the actin cytoskeleton by distinct signalling pathways in human neutrophils. Microbes Infect \n                           9, 47\u201354 (2007).","journal-title":"Microbes Infect"},{"key":"7564_CR29","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1046\/j.1365-2303.1998.00123.x","volume":"9","author":"C Hettlich","year":"1998","unstructured":"Hettlich, C., K\u00fcpper, T. H., Wehle, K. & Pfitzer, P. Aspergillus in the Papanicolaou stain: morphology, fluorescence and diagnostic feasibility. Cytopathology \n                           9, 381\u2013388 (1998).","journal-title":"Cytopathology"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-07564-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-07564-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-07564-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T08:16:19Z","timestamp":1671783379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-07564-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,8]]},"references-count":29,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["7564"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-07564-3","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,8]]},"assertion":[{"value":"23 August 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 June 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"7528"}}