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In the current study, transcriptional profiling was applied to analyse the variation in gene expression of <jats:italic>JS0135<\/jats:italic> and tildipirosin-resistant <jats:italic>JS32<\/jats:italic>. The growth curves showed that <jats:italic>JS32<\/jats:italic> had a higher growth rate but fewer bacteria than <jats:italic>JS0135<\/jats:italic>. The cell membranes of <jats:italic>JS32<\/jats:italic> and a resistant clinical isolate (<jats:italic>HB32<\/jats:italic>) were observed to be smoother than those of <jats:italic>JS0135<\/jats:italic>. From the comparative gene expression profile 349 up- and 113 downregulated genes were observed, covering 37 GO and 63 KEGG pathways which are involved in biological processes (11), cellular components (17), molecular function (9), cellular processes (1), environmental information processing (4), genetic information processing (9) and metabolism (49) affected in <jats:italic>JS32<\/jats:italic>. In addition, the relative overexpression of genes of the metabolism pathway (<jats:italic>HAPS_RS09315<\/jats:italic>, <jats:italic>HAPS_RS09320<\/jats:italic>), ribosomes (<jats:italic>HAPS_RS07815<\/jats:italic>) and ABC transporters (<jats:italic>HAPS_RS10945<\/jats:italic>) was detected, particularly the metabolism pathway, and verified with RT-qPCR. Collectively, the gene expression profile in connection with tildipirosin resistance factors revealed unique and highly resistant determinants of <jats:italic>H. parasuis<\/jats:italic> to macrolides that warrant further attention due to the significant threat of bacterial resistance.<\/jats:p>","DOI":"10.1038\/s41598-017-07972-5","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T08:31:39Z","timestamp":1501662699000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparative transcriptional profiling of tildipirosin-resistant and sensitive Haemophilus parasuis"],"prefix":"10.1038","volume":"7","author":[{"given":"Zhixin","family":"Lei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shulin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeed","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jincheng","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyue","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinsheng","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,8,8]]},"reference":[{"key":"7972_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetmic.2003.12.001","volume":"99","author":"S Oliveira","year":"2004","unstructured":"Oliveira, S. & Pijoan, C. 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