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The purpose of this study was to investigate potential bioactive antithrombotic and antiplatelet compounds from <jats:italic>O. chinensis sinuosa<\/jats:italic>. Five <jats:italic>N<\/jats:italic>-acetyldopamine dimers (<jats:bold>1<\/jats:bold>\u2013<jats:bold>5<\/jats:bold>) were isolated from <jats:italic>O. chinensis sinuosa<\/jats:italic> and compounds <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> were identified as new chemicals with chiral centers at H-2 and H-3 of the benzo-1,4-dioxane structure. Compounds <jats:bold>1<\/jats:bold>\u2013<jats:bold>4<\/jats:bold> were found to have both FXa and platelet aggregation inhibitory activities. These compounds inhibited the catalytic activity of FXa toward its synthetic substrate, S-2222, by noncompetitive inhibition, and inhibited platelet aggregation induced by ADP and U46619. Furthermore, compounds 1\u20134 showed enhanced antithrombotic effects, which were assessed using <jats:italic>in vivo<\/jats:italic> models of pulmonary embolism and arterial thrombosis. The isolated compounds also showed anticoagulant effects in mice. However, compounds 1\u20134 did not prolong bleeding time in mice, as shown by tail clipping. <jats:italic>N<\/jats:italic>-Acetyldopamine dimers, including two new stereoisomers <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold>, are novel antithrombotic compounds showing both FXa inhibition and antiplatelet aggregation activity with a low bleeding risk. Collectively, these results suggest that compounds 1\u20134 could serve as candidates and provide scaffolds for development of new antithrombotic drugs.<\/jats:p>","DOI":"10.1038\/s41598-017-08330-1","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T14:07:51Z","timestamp":1502114871000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of novel factor Xa inhibitors from Oxya chinensis sinuosa with anti-platelet aggregation activity"],"prefix":"10.1038","volume":"7","author":[{"given":"Wonhwa","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HeeSeung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Ae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joonhyeok","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Min","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Sam","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MinKyun","family":"Na","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Sup","family":"Bae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,11]]},"reference":[{"key":"8330_CR1","doi-asserted-by":"publisher","first-page":"266","DOI":"10.4103\/1947-2714.110430","volume":"5","author":"A Fares","year":"2013","unstructured":"Fares, A. 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