{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T06:05:55Z","timestamp":1788588355901,"version":"build-2803163510"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:00:00Z","timestamp":1502323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:00:00Z","timestamp":1502323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Progesterone soft capsules (brand name: Utrogestan) were demonstrated to be an effective oral alternative to prevent premature LH surges both in normal-ovulatory and polycystic ovarian syndrome (PCOS) patients. However, its safety in terms of neonatal outcomes is unclear. To evaluate whether Utrogestan use increase the risk of adverse neonatal outcomes compared with short protocol in patients undergoing IVF\/ICSI treatments in combination with frozen-thawed embryo transfer (FET), we performed a retrospective analysis including 1008 FET cycles, with embryos originated from either Utrogestan\u2009+\u2009hMG protocol (n\u2009=\u2009499), or short protocol (n\u2009=\u2009509), which led to 546 live-born infants. The neonatal characteristics regarding preterm birth (PTB), low birth weight (LBW), gestational age and mode of delivery were comparable in the two groups. The incidence of live-birth defect was 0.68% (2\/293) in the Utrogestan\u2009+\u2009hMG protocol compared with 0.79% (2\/253) in the short protocol. No early neonatal death or intrauterine death were recorded in either group. To date, the data do not indicate an elevated rate of abnormality at birth after progesterone use during ovarian stimulation but further study with larger populations is needed to confirm these results.<\/jats:p>","DOI":"10.1038\/s41598-017-08472-2","type":"journal-article","created":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T14:50:52Z","timestamp":1501858252000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Comparison of neonatal outcomes following progesterone use during ovarian stimulation with frozen-thawed embryo transfer"],"prefix":"10.1038","volume":"7","author":[{"given":"Xiuxian","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjuan","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonglun","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,10]]},"reference":[{"key":"8472_CR1","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1016\/j.fertnstert.2013.10.017","volume":"101","author":"X Yang","year":"2014","unstructured":"Yang, X., Li, Y., Li, C. & Zhang, W. Current overview of pregnancy complications and live-birth outcome of assisted reproductive technology in mainland China. Fertil Steril. \n                           101, 385\u2013391 (2014).","journal-title":"Fertil Steril."},{"key":"8472_CR2","doi-asserted-by":"publisher","first-page":"1433","DOI":"10.1093\/humrep\/deq072","volume":"25","author":"M Bonduelle","year":"2010","unstructured":"Bonduelle, M., Obery\u00e9, J., Mannaerts, B. & Devroey, P. Large prospective, pregnancy and infant follow-up trial assures the health of 1000 fetuses conceived after treatment with the GnRH antagonist ganirelix during controlled ovarian stimulation. Hum Reprod. \n                           25, 1433\u20131440 (2010).","journal-title":"Hum Reprod."},{"key":"8472_CR3","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1055\/s-2002-36713","volume":"20","author":"AC van Loenen","year":"2002","unstructured":"van Loenen, A. C., Huirne, J. A., Schats, R., Hompes, P. G. & Lambalk, C. B. GnRH agonists, antagonists, and assisted conception. Semin Reprod Med. \n                           20, 349\u2013364 (2002).","journal-title":"Semin Reprod Med."},{"key":"8472_CR4","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.1016\/j.fertnstert.2003.07.002","volume":"80","author":"E Bosch","year":"2003","unstructured":"Bosch, E. et al. Premature luteinization during gonadotropin-releasing hormone antagonist cycles and its relationship with in vitro fertilization outcome. Fertil Steril. \n                           80, 1444\u20131449 (2003).","journal-title":"Fertil Steril."},{"key":"8472_CR5","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1210\/er.2005-0015","volume":"27","author":"NS Macklon","year":"2006","unstructured":"Macklon, N. S., Stouffer, R. L., Giudice, L. C. & Fauser, B. C. The science behind 25 years of ovarian stimulation for in vitro fertilization. Endocr Rev. \n                           27, 170\u2013207 (2006).","journal-title":"Endocr Rev."},{"key":"8472_CR6","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1093\/humrep\/del340","volume":"22","author":"I Cedrin-Durnerin","year":"2007","unstructured":"Cedrin-Durnerin, I. et al. Effects of oral contraceptive, synthetic progestogen or natural estrogen pre-treatments on the hormonal profile and the antral follicle cohort before GnRH antagonist protocol. Hum Reprod. \n                           22, 109\u2013116 (2007).","journal-title":"Hum Reprod."},{"key":"8472_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3109\/09513590009167652","volume":"14","author":"GS Letterie","year":"2000","unstructured":"Letterie, G. S. Inhibition of gonadotropin surge by a brief mid-cycle regimen of ethinyl estradiol and norethindrone: possible role in in vitro fertilization. Gynecol Endocrinol. \n                           14, 1\u20134 (2000).","journal-title":"Gynecol Endocrinol."},{"key":"8472_CR8","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.fertnstert.2014.05.027","volume":"102","author":"KM Wong","year":"2014","unstructured":"Wong, K. M., Mastenbroek, S. & Repping, S. Cryopreservation of human embryos and its contribution to in vitro fertilization success rates. Fertil Steril. \n                           102, 19\u201326 (2014).","journal-title":"Fertil Steril."},{"key":"8472_CR9","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1016\/j.fertnstert.2014.07.1245","volume":"102","author":"B Huang","year":"2014","unstructured":"Huang, B. et al. Is frozen embryo transfer cycle associated with a significantly lower incidence of ectopic pregnancy? An analysis of more than 30,000 cycles. Fertil Steril. \n                           102, 1345\u20131349 (2014).","journal-title":"Fertil Steril."},{"key":"8472_CR10","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.fertnstert.2012.09.003","volume":"99","author":"M Roque","year":"2013","unstructured":"Roque, M. et al. Fresh embryo transfer versus frozen embryo transfer in in vitro fertilization cycles: a systematic review and meta-analysis. Fertil Steril. \n                           99, 156\u2013162 (2013).","journal-title":"Fertil Steril."},{"key":"8472_CR11","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.fertnstert.2013.09.007","volume":"101","author":"Y Kuang","year":"2014","unstructured":"Kuang, Y. et al. Luteal-phase ovarian stimulation is feasible for producing competent oocytes in women undergoing in vitro fertilization\/intracytoplasmic sperm injection treatment, with optimal pregnancy outcomes in frozen-thawed embryo transfer cycles. Fertil Steril. \n                           101, 105\u2013111 (2014).","journal-title":"Fertil Steril."},{"key":"8472_CR12","doi-asserted-by":"publisher","first-page":"1194","DOI":"10.1016\/j.fertnstert.2015.02.020","volume":"103","author":"H Chen","year":"2015","unstructured":"Chen, H. et al. Comparison of live-birth defects after luteal-phase ovarian stimulation vs. conventional ovarian stimulation for in vitro fertilization and vitrified embryo transfer cycles. Fertil Steril. \n                           103, 1194\u20131201 (2015).","journal-title":"Fertil Steril."},{"key":"8472_CR13","doi-asserted-by":"publisher","first-page":"e909","DOI":"10.1097\/MD.0000000000000909","volume":"94","author":"XX Zhu","year":"2015","unstructured":"Zhu, X. X., Zhang, X. L. & Fu, Y. L. Utrogestan as an effective oral alternative for preventing premature luteinizing hormone surges in women undergoing controlled ovarian hyperstimulation for in vitro fertilization. Medicine (Baltimore). \n                           94, e909 (2015).","journal-title":"Medicine (Baltimore)."},{"key":"8472_CR14","first-page":"384","volume":"35","author":"XX Zhu","year":"2015","unstructured":"Zhu, X. X., Zhang, X. L. & Fu, Y. L. Effect of progesterone used to prevent LH surges in controlled ovarian stimulation. Reprod Contra. \n                           35, 384\u2013388 (2015).","journal-title":"Reprod Contra."},{"key":"8472_CR15","doi-asserted-by":"publisher","first-page":"e4193","DOI":"10.1097\/MD.0000000000004193","volume":"95","author":"XX Zhu","year":"2016","unstructured":"Zhu, X. X., Ye, H. J. & Fu, Y. L. The Utrogestan and hMG protocol in patients with polycystic ovarian syndrome undergoing controlled ovarian hyperstimulation during IVF\/ICSI treatments. Medicine (Baltimore). \n                           95, e4193 (2016).","journal-title":"Medicine (Baltimore)."},{"key":"8472_CR16","doi-asserted-by":"crossref","unstructured":"Zhu, X. X., Ye, H. J. and Fu, Y. L. The use of Utrogestan during controlled ovarian hyperstimulation in normally ovulating women undergoing in vitro fertilization or intracytoplasmic sperm injection treatments in combination with a \u201cfreeze all\u201d strategy: a randomized controlled dose-finding study of 100 mg versus 200 mg. Fertil Steril. (Epub ahead of print) (2016).","DOI":"10.1016\/j.fertnstert.2016.10.030"},{"key":"8472_CR17","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1093\/humupd\/dms044","volume":"19","author":"A Pinborg","year":"2013","unstructured":"Pinborg, A. et al. Why do singletons conceived after assisted reproduction technology have adverse perinatal outcome? Systematic review and meta-analysis. Hum Reprod Update. \n                           19, 87\u2013104 (2013).","journal-title":"Hum Reprod Update."},{"key":"8472_CR18","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.ejogrb.2009.09.019","volume":"148","author":"SD McDonald","year":"2010","unstructured":"McDonald, S. D. et al. Preterm birth and low birth weight among in vitro fertilization twins: a systematic review and meta-analyses. Eur J Obstet Gynecol Reprod Biol. \n                           148, 105\u2013113 (2010).","journal-title":"Eur J Obstet Gynecol Reprod Biol."},{"key":"8472_CR19","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1093\/humupd\/dmt006","volume":"19","author":"M Hansen","year":"2013","unstructured":"Hansen, M., Kurinczuk, J. J., Milne, E., de Klerk, N. & Bower, C. Assisted reproductive technology and birth defects: a systematic review and meta-analysis. Hum Reprod Update. \n                           19, 330\u2013353 (2013).","journal-title":"Hum Reprod Update."},{"key":"8472_CR20","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.fertnstert.2014.09.037","volume":"103","author":"S Jackson","year":"2015","unstructured":"Jackson, S. et al. Pregnancy outcomes in very advanced maternal age pregnancies: the impact of assisted reproductive technology. Fertil Steril. \n                           103, 76\u201380 (2015).","journal-title":"Fertil Steril."},{"key":"8472_CR21","doi-asserted-by":"crossref","unstructured":"Zhong, X. et al. Effect of parental physiological conditions and assisted reproductive technologies on the pregnancy and birth outcomes in infertile patients. Oncotarget. (Epub ahead of print) (2016).","DOI":"10.18632\/oncotarget.12553"},{"key":"8472_CR22","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1055\/s-2007-992927","volume":"26","author":"CM Boomsma","year":"2008","unstructured":"Boomsma, C. M., Fauser, B. C. & Macklon, N. S. Pregnancy complications in women with polycystic ovarian syndrome. Semin Reprod Med. \n                           26, 72\u201384 (2008).","journal-title":"Semin Reprod Med."},{"key":"8472_CR23","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1016\/j.fertnstert.2016.04.021","volume":"106","author":"X Wang","year":"2016","unstructured":"Wang, X. et al. Effects of female and male body mass indices on the treatment outcomes and neonatal birth weights associated with in vitro fertilization\/intracytoplasmic sperm injection treatment in China. Fertil Steril. \n                           106, 460\u2013466 (2016).","journal-title":"Fertil Steril."},{"key":"8472_CR24","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1016\/j.fertnstert.2016.06.021","volume":"106","author":"AL Wennberg","year":"2016","unstructured":"Wennberg, A. L. et al. Effect of maternal age on maternal and neonatal outcomes after assisted reproductive technology. Fertil Steril. \n                           106, 1142\u20131149 (2016).","journal-title":"Fertil Steril."},{"key":"8472_CR25","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1093\/humrep\/dev076","volume":"30","author":"SK Sunkara","year":"2015","unstructured":"Sunkara, S. K., La Marca, A., Seed, P. T. & Khalaf, Y. Increased risk of preterm birth and low birthweight with very high number of oocytes following IVF: an analysis of 65 868 singleton live birth outcomes. Hum Reprod. \n                           30, 1473\u20131480 (2015).","journal-title":"Hum Reprod."},{"key":"8472_CR26","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.fertnstert.2016.03.049","volume":"106","author":"BS Shapiro","year":"2016","unstructured":"Shapiro, B. S., Daneshmand, S. T., Bedient, C. E. & Garner, F. C. Comparison of birth weights in patients randomly assigned to fresh or frozen-thawed embryo transfer. Fertil Steril. \n                           106, 317\u2013321 (2016).","journal-title":"Fertil Steril."},{"key":"8472_CR27","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1093\/humrep\/des377","volume":"28","author":"J Fedder","year":"2013","unstructured":"Fedder, J., Loft, A., Parner, E. T., Rasmussen, S. & Pinborg, A. Neonatal outcome and congenital malformations in children born after ICSI with testicular or epididymal sperm: a controlled national cohort study. Hum Reprod. \n                           28, 230\u2013240 (2013).","journal-title":"Hum Reprod."},{"key":"8472_CR28","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1016\/j.fertnstert.2015.02.026","volume":"103","author":"B Huang","year":"2015","unstructured":"Huang, B. et al. Neonatal outcomes after early rescue intracytoplasmic sperm injection: an analysis of a 5-year period. Fertil Steril. \n                           103, 1432\u20131437 (2015).","journal-title":"Fertil Steril."},{"key":"8472_CR29","doi-asserted-by":"publisher","first-page":"2217","DOI":"10.1210\/jc.2013-3362","volume":"99","author":"XL Hu","year":"2014","unstructured":"Hu, X. L. et al. High maternal serum estradiol environment in the first trimester is associated with the increased risk of small-for-gestational-age birth. J Clin Endocrinol Metab. \n                           99, 2217\u20132224 (2014).","journal-title":"J Clin Endocrinol Metab."},{"key":"8472_CR30","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1093\/humrep\/deu362","volume":"30","author":"CA Venetis","year":"2015","unstructured":"Venetis, C. A. et al. Estimating the net effect of progesterone elevation on the day of hCG on live birth rates after IVF: a cohort analysis of 3296 IVF cycles. Hum Reprod. \n                           30, 684\u2013691 (2015).","journal-title":"Hum Reprod."},{"key":"8472_CR31","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1093\/humupd\/dmm007","volume":"13","author":"CA Venetis","year":"2007","unstructured":"Venetis, C. A. et al. Is P elevation on the day of human chorionic gonadotrophin administration associated with the probability of pregnancy in in vitro fertilization? A systematic review and meta-analysis. Hum Reprod Update. \n                           13, 343\u2013355 (2007).","journal-title":"Hum Reprod Update."},{"key":"8472_CR32","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1016\/j.fertnstert.2010.12.065","volume":"95","author":"EA Elgindy","year":"2011","unstructured":"Elgindy, E. A. Progesterone level and progesterone\/estradiol ratio on the day of hCG administration: detrimental cutoff levels and new treatment strategy. Fertil Steril. \n                           95, 1639\u20131644 (2011).","journal-title":"Fertil Steril."},{"key":"8472_CR33","doi-asserted-by":"publisher","first-page":"2092","DOI":"10.1093\/humrep\/deq125","volume":"25","author":"E Bosch","year":"2010","unstructured":"Bosch, E. et al. Circulating progesterone levels and ongoing pregnancy rates in controlled ovarian stimulation cycles for in vitro fertilization: analysis of over 4000 cycles. Hum Reprod. \n                           25, 2092\u20132100 (2010).","journal-title":"Hum Reprod."},{"key":"8472_CR34","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1080\/09513590701403629","volume":"23","author":"KM Seow","year":"2007","unstructured":"Seow, K. M. et al. Subtle progesterone rise in the single-dose gonadotropin-releasing hormone antagonist (cetrorelix) stimulation protocol in patients undergoing in vitro fertilization or intracytoplasmic sperm injection cycles. Gynecol Endocrinol. \n                           23, 338\u2013342 (2007).","journal-title":"Gynecol Endocrinol."},{"key":"8472_CR35","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1007\/s10815-009-9309-7","volume":"26","author":"HA Saleh","year":"2009","unstructured":"Saleh, H. A., Omran, M. S. E. A. & Draz, M. Does subtle progesterone rise on the day of HCG affect pregnancy rate in long agonist ICSI cycles? J Assist Reprod Genet. \n                           26, 239\u2013242 (2009).","journal-title":"J Assist Reprod Genet."},{"key":"8472_CR36","doi-asserted-by":"publisher","first-page":"1506","DOI":"10.1093\/oxfordjournals.humrep.a138288","volume":"8","author":"RS Legro","year":"1993","unstructured":"Legro, R. S., Ary, B. A., Paulson, R. J., Stanczyk, F. Z. & Sauer, M. V. Pregnancy: premature luteinization as detected by elevated serum progesterone is associated with a higher pregnancy rate in donor oocyte in-vitro fertilization. Hum Reprod. \n                           8, 1506\u20131511 (1993).","journal-title":"Hum Reprod."},{"key":"8472_CR37","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1093\/humrep\/14.3.601","volume":"14","author":"N Doldi","year":"1999","unstructured":"Doldi, N. et al. Elevated serum progesterone on the day of HCG administration in IVF is associated with a higher pregnancy rate in polycystic ovary syndrome. Hum Reprod. \n                           14, 601\u2013605 (1999).","journal-title":"Hum Reprod."},{"key":"8472_CR38","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1093\/humupd\/dmt014","volume":"19","author":"CA Venetis","year":"2013","unstructured":"Venetis, C. A., Kolibianakis, E. M., Bosdou, J. K. & Tarlatzis, B. C. Progesterone elevation and probability or pregnancy after IVF: a systematic review and meta-analysis of over 60 000 cycles. Hum Reprod Update. \n                           19, 433\u2013457 (2013).","journal-title":"Hum Reprod Update."},{"key":"8472_CR39","doi-asserted-by":"publisher","first-page":"1813","DOI":"10.1093\/humrep\/der126","volume":"26","author":"E Labarta","year":"2011","unstructured":"Labarta, E. et al. Endometrial receptivity is affected in women with high circulating progesterone levels at the end of the follicular phase: a functional genomics analysis. Hum Reprod. \n                           26, 1813\u20131825 (2011).","journal-title":"Hum Reprod."},{"key":"8472_CR40","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.rbmo.2010.11.002","volume":"22","author":"V Van","year":"2011","unstructured":"Van, V. et al. Progesterone rise on HCG day in GnRH antagonist\/rFSH stimulated cycles affects endometrial gene expression. Reprod Biomed Online. \n                           22, 263\u2013271 (2011).","journal-title":"Reprod Biomed Online."},{"key":"8472_CR41","first-page":"E14","volume":"82","author":"RL Krisher","year":"2004","unstructured":"Krisher, R. L. The effect of oocyte quality on development. J Anim Sci. \n                           82, E14\u201323 (2004).","journal-title":"J Anim Sci."},{"key":"8472_CR42","first-page":"20","volume":"1","author":"M Salehnia","year":"2003","unstructured":"Salehnia, M. Progesterone shifts the pinopodes expression of mouse endometrium to pre-implantation time after ovarian hyperstimulation. Iran J Reprod Med. \n                           1, 20\u201323 (2003).","journal-title":"Iran J Reprod Med."},{"key":"8472_CR43","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/0093-691X(82)90100-5","volume":"18","author":"Y Fukui","year":"1982","unstructured":"Fukui, Y., Fukushima, M., Terawaki, Y. & Ono, H. Effect of gonadotropins, steroids and culture media on bovine oocyte maturation in vitro. Theriogenology. \n                           18, 161\u2013175 (1982).","journal-title":"Theriogenology."},{"key":"8472_CR44","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1530\/reprod\/119.2.261","volume":"119","author":"CC Silva","year":"2000","unstructured":"Silva, C. C. & Knight, P. G. Effects of androgens, progesterone and their antagonists on the developmental competence of in vitro matured bovine oocytes. J Reprod Fertil. \n                           119, 261\u2013269 (2000).","journal-title":"J Reprod Fertil."},{"key":"8472_CR45","first-page":"83","volume":"51","author":"DA Hewitt","year":"1997","unstructured":"Hewitt, D. A. & England, G. C. Effect of preovulatory endocrine events upon maturation of oocytes of domestic bitches. J Reprod Fertil Suppl. \n                           51, 83\u201391 (1997).","journal-title":"J Reprod Fertil Suppl."},{"key":"8472_CR46","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1095\/biolreprod.109.082354","volume":"83","author":"F Carter","year":"2010","unstructured":"Carter, F. et al. Effect of elevated circulating progesterone concentration on bovine blastocyst development and global transcriptome following endoscopic transfer of in vitro produced embryos to the bovine oviduct. Biol Reprod. \n                           83, 707\u2013719 (2010).","journal-title":"Biol Reprod."},{"key":"8472_CR47","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1530\/rep.0.1260501","volume":"126","author":"LA Willingham-Rocky","year":"2003","unstructured":"Willingham-Rocky, L. A., Hinrichs, K., Westhusin, M. E. & Kraemer, D. C. Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction. \n                           126, 501\u2013508 (2003).","journal-title":"Reproduction."},{"key":"8472_CR48","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1016\/j.theriogenology.2006.01.008","volume":"66","author":"CI Vannucchi","year":"2006","unstructured":"Vannucchi, C. I., de Oliveira, C. M., Marques, M. G., Assumpcao, M. E. & Visintin, J. A. In vitro canine oocyte nuclear maturation in homologous oviductal cell co-culture with hormone- supplemented media. Theriogenology. \n                           66, 1677\u20131681 (2006).","journal-title":"Theriogenology."},{"key":"8472_CR49","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1002\/mrd.1120230304","volume":"23","author":"X Zhang","year":"1989","unstructured":"Zhang, X. & Armstrong, D. T. Effects of follicle-stimulating hormone and ovarian steroids during in vitro meiotic maturation on fertilization of rat oocytes. Gamete Res. \n                           23, 267\u2013277 (1989).","journal-title":"Gamete Res."},{"key":"8472_CR50","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1002\/mrd.1080270209","volume":"27","author":"PM Morgan","year":"1990","unstructured":"Morgan, P. M., Boatman, D. E. & Bavister, B. D. Relationships between follicular fluid steroid hormone concentrations, oocyte maturity, in vitro fertilization and embryonic development in the rhesus monkey. Mol Reprod Dev. \n                           27, 145\u2013151 (1990).","journal-title":"Mol Reprod Dev."},{"key":"8472_CR51","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1016\/j.fertnstert.2010.08.024","volume":"95","author":"J Yan","year":"2011","unstructured":"Yan, J. et al. Birth defects after assisted reproductive technologies in China: analysis of 15,405 offspring in seven centers (2004 to 2008). Fertil Steril. \n                           95, 458\u2013460 (2011).","journal-title":"Fertil Steril."},{"key":"8472_CR52","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1093\/humrep\/dep364","volume":"25","author":"JL Halliday","year":"2010","unstructured":"Halliday, J. L. et al. Increased risk of blastogenesis birth defects, arising in the first 4 weeks of pregnancy, after assisted reproductive technologies. Hum Reprod. \n                           25, 59\u201365 (2010).","journal-title":"Hum Reprod."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08472-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08472-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08472-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T08:29:11Z","timestamp":1671784151000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08472-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,10]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["8472"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-08472-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,10]]},"assertion":[{"value":"30 December 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 July 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"7835"}}