{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T13:43:14Z","timestamp":1792935794073,"version":"4.5.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T00:00:00Z","timestamp":1503273600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T00:00:00Z","timestamp":1503273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated possibility of predicting whether blooms, if they occur, would be formed of microcystin-producing cyanobacteria. DGGE analysis of 16S-ITS and <jats:italic>mcy<\/jats:italic>A genes revealed that only <jats:italic>Planktothrix<\/jats:italic> and <jats:italic>Microcystis<\/jats:italic> possessed <jats:italic>mcy<\/jats:italic>-genes and <jats:italic>Planktothrix<\/jats:italic> was the main microcystin producer. qPCR analysis revealed that the proportion of cells with <jats:italic>mcy<\/jats:italic>-genes in <jats:italic>Planktothrix<\/jats:italic> populations was almost 100%. Microcystin concentration correlated with the number of potentially toxic and total <jats:italic>Planktothrix<\/jats:italic> cells and the proportion of <jats:italic>Planktothrix<\/jats:italic> within all cyanobacteria, but not with the proportion of cells with <jats:italic>mcy<\/jats:italic>-genes in total <jats:italic>Planktothrix<\/jats:italic>. The share of <jats:italic>Microcystis<\/jats:italic> cells with <jats:italic>mcy<\/jats:italic>-genes was low and variable in time. Neither the number of <jats:italic>mcy<\/jats:italic>-possessing cells, nor the proportion of these cells in total <jats:italic>Microcystis<\/jats:italic>, correlated with the concentration of microcystins. This suggests that it is possible to predict whether the bloom in the Masurian Lakes will be toxic based on <jats:italic>Planktothrix<\/jats:italic> occurrence. Two species of toxin producing <jats:italic>Planktothrix<\/jats:italic>, <jats:italic>P. agardhii<\/jats:italic> and <jats:italic>P. rubescens<\/jats:italic>, were identified by phylogenetic analysis of 16S-ITS. Based on morphological and ecological features, the toxic <jats:italic>Planktothrix<\/jats:italic> was identified as <jats:italic>P. agardhii<\/jats:italic>. However, the very high proportion of cells with <jats:italic>mcy<\/jats:italic>-genes suggests <jats:italic>P. rubescens<\/jats:italic>. Our study reveals the need of universal primers for <jats:italic>mcy<\/jats:italic>A genes from environment.<\/jats:p>","DOI":"10.1038\/s41598-017-08701-8","type":"journal-article","created":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T09:52:31Z","timestamp":1502358751000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities"],"prefix":"10.1038","volume":"7","author":[{"given":"Aleksandra","family":"Bukowska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Kali\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Koper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Kostrzewska-Szlakowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Kwiatowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Mazur-Marzec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Jasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,21]]},"reference":[{"key":"8701_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1023\/A:1004155810302","volume":"438","author":"MT Dokulil","year":"2000","unstructured":"Dokulil, M. T. & Teubner, K. Cyanobacterial dominance in lakes. Hydrobiologia \n                           438, 1\u201312, doi:10.1023\/A:1004155810302 (2000).","journal-title":"Hydrobiologia"},{"key":"8701_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-0-387-75865-7_1","volume":"619","author":"HK Hudnell","year":"2008","unstructured":"Hudnell, H. K. Proceedings of the interagency, international symposium on cyanobacterial harmful algal blooms (ISOC-HAB): state of the science and research needs. Adv Exp Med Biol \n                           619, 1\u2013924 (2008).","journal-title":"Adv Exp Med Biol"},{"key":"8701_CR3","unstructured":"Merilouto, J. & Codd, G. A. Toxic: Cyanobacterial Monitoring and Cyanotoxin Analysis (\u00c5bo Akademi University Press, \u00c5bo, Finland 2005)."},{"key":"8701_CR4","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1126\/science.1155398","volume":"320","author":"HW Paerl","year":"2008","unstructured":"Paerl, H. W. & Huisman, J. Climate. Blooms like it hot. Science \n                           320, 57\u201358, doi:10.1126\/science.1155398 (2008).","journal-title":"Blooms like it hot. Science"},{"key":"8701_CR5","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1126\/science1245276","volume":"342","author":"HW Paerl","year":"2013","unstructured":"Paerl, H. W. & Otten, T. G. Environmental science. Blooms bite the hand that feeds them. Science \n                           342, 433\u2013434, doi:10.1126\/science1245276 (2013).","journal-title":"Blooms bite the hand that feeds them. Science"},{"key":"8701_CR6","first-page":"481","volume":"27","author":"I Spodniewska","year":"1979","unstructured":"Spodniewska, I. Phytoplankton as the indicator of lake eutrophication. 2. Summer situation in 25 Masurian lakes in 1976. Ekol pol \n                           27, 481\u201349 (1979).","journal-title":"Ekol pol"},{"key":"8701_CR7","first-page":"353","volume":"31","author":"I Spodniewska","year":"1983","unstructured":"Spodniewska, I. Ecological characteristics of lakes in north-eastern Poland versus their trophic gradient VI. The phytoplankton of 43 lakes. Ekol pol \n                           31, 353\u2013381 (1983).","journal-title":"Ekol pol"},{"key":"8701_CR8","first-page":"209","volume":"35","author":"A Napi\u00f3rkowska-Krzebietke","year":"2006","unstructured":"Napi\u00f3rkowska-Krzebietke, A. & Hutorowicz, A. Long-term changes of phytoplankton in Lake Niegocin, in the Masurian Lake Region, Poland. Oceanol Hydrobiol Stud \n                           35, 209\u2013226 (2006).","journal-title":"Oceanol Hydrobiol Stud"},{"key":"8701_CR9","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/2046-9063-10-2","volume":"10","author":"A Bukowska","year":"2014","unstructured":"Bukowska, A., Bielczy\u0144ska, A., Karnkowska, A., Chr\u00f3st, R. J. & Jasser, I. Molecular (PCR-DGGE) versus morphological approach: analysis of taxonomic composition of potentially toxic cyanobacteria in freshwater lakes. Aquat Biosyst \n                           10, 2\u201311, doi:10.1186\/2046-9063-10-2 (2014).","journal-title":"Aquat Biosyst"},{"key":"8701_CR10","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1515\/ohs-2015-0010","volume":"44","author":"A Napi\u00f3rkowska-Krzebietke","year":"2015","unstructured":"Napi\u00f3rkowska-Krzebietke, A. Cyanobacterial bloom intensity in the ecologically relevant state of lakes \u2013 an approach to Water Framework Directive implementation. Oceanol Hydrobiol Stud \n                           44, 97\u2013108, doi:10.1515\/ohs-2015-0010 (2015).","journal-title":"Oceanol Hydrobiol Stud"},{"key":"8701_CR11","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1608\/FRJ-2.1.2","volume":"2","author":"R Kurmayer","year":"2009","unstructured":"Kurmayer, R. & Christiansen, G. The genetic basis of toxin production in cyanobacteria. Freshw Rev \n                           2, 31\u201350, doi:10.1608\/FRJ-2.1.2 (2009).","journal-title":"Freshw Rev"},{"key":"8701_CR12","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/j.1574-6968.2011.02222.x","volume":"317","author":"R Kurmayer","year":"2011","unstructured":"Kurmayer, R., Schober, E., Tonk, L., Visser, P. M. & Christiansen, G. Spatial divergence in the proportions of genes encoding toxic peptide synthesis among populations of the cyanobacterium Planktothrix in European lakes. FEMS Microbiol Lett \n                           317, 127\u2013137, doi:10.1111\/j.1574-6968.2011.02222.x (2011).","journal-title":"FEMS Microbiol Lett"},{"issue":"5","key":"8701_CR13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0125353","volume":"10","author":"LJ Beversdorf","year":"2015","unstructured":"Beversdorf, L. J., Chaston, S. D., Miller, T. R. & McMahon, K. D. Microcystin mcyA and mcyE Gene Abundances Are Not Appropriate Indicators of Microcystin Concentrations in Lakes. PLoS ONE \n                           10(5), e0125353, doi:10.1371\/journal.pone.0125353 (2015).","journal-title":"PLoS ONE"},{"key":"8701_CR14","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1002\/tox.20138","volume":"20","author":"J Mankiewicz","year":"2005","unstructured":"Mankiewicz, J. et al. Hepatotoxic cyanobacterial blooms in the lakes of northern Poland. Environ Toxicol \n                           20, 499\u2013506, doi:10.1002\/tox.20138 (2005).","journal-title":"Environ Toxicol"},{"key":"8701_CR15","unstructured":"Kom\u00e1rek, J., Ka\u0161tovsk\u00fd, J., Mare\u0161, J. & Johansen, J. R. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach (2014)."},{"key":"8701_CR16","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1127\/archiv-hydrobiol\/145\/1999\/147","volume":"145","author":"J Fastner","year":"1999","unstructured":"Fastner, J. et al. Characterization and diversity of microcystins in natural blooms and strains of the genera Microcystis and Planktothrix from German freshwaters. Arch Hydrobiol \n                           145, 147\u2013163 (1999).","journal-title":"Arch Hydrobiol"},{"key":"8701_CR17","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1007\/s00203-003-0605-9","volume":"180","author":"M Hisbergues","year":"2003","unstructured":"Hisbergues, M., Christiansen, G., Rouhiainen, L., Sivonen, K. & B\u00f6rner, T. PCR-based identification of microcystin-producing genotypes of different cyanobacterial genera. Arch Microbiol \n                           180, 402\u2013410, doi:10.1007\/s00203-003-0605-9 (2003).","journal-title":"Arch Microbiol"},{"key":"8701_CR18","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.envpol.2007.12.027","volume":"156","author":"Y Xu","year":"2008","unstructured":"Xu, Y. et al. Non-microcystin producing Microcystis wesenbergii (Kom\u00e1rek) Kom\u00e1rek (Cyanobacteria) representing a main waterbloom-forming species in Chinese waters. Environ pollut \n                           156, 162\u2013167, doi:10.1016\/j.envpol.2007.12.027 (2008).","journal-title":"Environ pollut"},{"key":"8701_CR19","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1073\/pnas.0304489101","volume":"101","author":"A Rantala","year":"2004","unstructured":"Rantala, A. et al. Phylogenetic evidence for the early evolution of microcystin synthesis. Proc Natl Acad Sci USA \n                           101, 568\u2013573, doi:10.1073\/pnas.0304489101 (2004).","journal-title":"Proc Natl Acad Sci USA"},{"key":"8701_CR20","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s11274-009-0228-7","volume":"26","author":"R Prasanna","year":"2010","unstructured":"Prasanna, R., Gupta, V., Natarajan, C. & Chaudhary, V. Bioprospecting for genes involved in the production of chitosanases and microcystin-like compounds in Anabaena strains. World J Microbiol Biotechnol \n                           26, 717\u2013724, doi:10.1007\/s11274-009-0228-7 (2010).","journal-title":"World J Microbiol Biotechnol"},{"key":"8701_CR21","doi-asserted-by":"publisher","unstructured":"Gupta, V. et al. Analyses of diversity among fungicidal Anabaena strains. J Appl Phycol, 1395\u20131405 doi:10.1007\/s10811-012-9793-5 (2012).","DOI":"10.1007\/s10811-012-9793-5"},{"key":"8701_CR22","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1111\/j.1574-6968.2007.00751.x","volume":"272","author":"LL Richardson","year":"2007","unstructured":"Richardson, L. L. et al. The presence of the cyanobacterial toxin microcystin in black band disease of corals. FEMS Microbiol Lett \n                           272, 182\u2013187, doi:10.1111\/j.1574-6968.2007.00751.x (2007).","journal-title":"FEMS Microbiol Lett"},{"key":"8701_CR23","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1007\/s00203-011-0704-y","volume":"193","author":"A Fathalli","year":"2011","unstructured":"Fathalli, A. et al. Genetic variability of the invasive cyanobacteria Cylindrospermopsis raciborskii from Bir M\u2019cherga reservoir (Tunisia). Arch Microbiol \n                           193, 595\u2013604, doi:10.1007\/s00203-011-0704-y (2011).","journal-title":"Arch Microbiol"},{"key":"8701_CR24","doi-asserted-by":"publisher","first-page":"5756","DOI":"10.1128\/AEM.70.10.5756-5763.2004","volume":"70","author":"I Oksanen","year":"2004","unstructured":"Oksanen, I. et al. Discovery of rare and highly toxic microcystins from lichen-associated cyanobacterium Nostoc sp. Appl Environ Microbiol \n                           70, 5756\u20135763, doi:10.1128\/AEM.70.10.5756-5763.2004 (2004).","journal-title":"Appl Environ Microbiol"},{"key":"8701_CR25","first-page":"719","volume":"22","author":"M Nebaeus","year":"1984","unstructured":"Nebaeus, M. Algal water-blooms under ice-cover. Verh Internat Verein Limnol \n                           22, 719\u2013724 (1984).","journal-title":"Verh Internat Verein Limnol"},{"key":"8701_CR26","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.hal.2003.12.006","volume":"4","author":"S Jacquet","year":"2005","unstructured":"Jacquet, S. et al. The proliferation of the toxic cyanobacterium Planktothrix rubescens following restoration of the largest natural French lake (Lac du Bourget). Harm Algae \n                           4, 651\u2013672, doi:10.1016\/j.hal.2003.12.006 (2005).","journal-title":"Harm Algae"},{"key":"8701_CR27","doi-asserted-by":"publisher","first-page":"358","DOI":"10.2478\/s13545-013-0093-8ran","volume":"42","author":"J Kobos","year":"2013","unstructured":"Kobos, J. et al. Cyanobacteria and cyanotoxins in Polish freshwater bodies. Ocean Hydrobiol Stud \n                           42, 358\u2013378, doi:10.2478\/s13545-013-0093-8ran (2013).","journal-title":"Ocean Hydrobiol Stud"},{"key":"8701_CR28","doi-asserted-by":"publisher","first-page":"6101","DOI":"10.1128\/AEM.01058-06","volume":"72","author":"A Rantala","year":"2006","unstructured":"Rantala, A. et al. Detection of microcystin-producing cyanobacteria in Finnish lakes with genus-specific microcystin synthetase gene E (mcyE) PCR and associations with environmental factors. Appl Environ Microbiol \n                           72, 6101\u20136110, doi:10.1128\/AEM.01058-06 (2006).","journal-title":"Appl Environ Microbiol"},{"key":"8701_CR29","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1007\/s00203-014-1008-9","volume":"196","author":"M Grabowska","year":"2014","unstructured":"Grabowska, M., Kobos, J., Toru\u0144ska-Sitarz, A. & Mazur-Marzec, H. Non-ribosomal peptides produced by Planktothrix agardhii from Siemian\u00f3wka Dam Reservoir SDR (northeast Poland). Arch Microbiol \n                           196, 697\u2013707, doi:10.1007\/s00203-014-1008-9 (2014).","journal-title":"Arch Microbiol"},{"key":"8701_CR30","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1080\/09670260802158659","volume":"44","author":"S Kosol","year":"2009","unstructured":"Kosol, S., Schmidt, J. & Kurmayer, R. Variation in peptide net production and growth among strains of the toxic cyanobacterium Planktothrix spp. Eur J Phycol \n                           44, 49\u201362, doi:10.1080\/09670260802158659 (2009).","journal-title":"Eur J Phycol"},{"key":"8701_CR31","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1099\/00207713-52-5-1577","volume":"52","author":"S Suda","year":"2002","unstructured":"Suda, S. et al. Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria. Int J Syst Evol Microbiol \n                           52, 1577\u20131595, doi:10.1099\/00207713-52-5-1577 (2002).","journal-title":"Int J Syst Evol Microbiol"},{"key":"8701_CR32","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.4319\/lo.2008.53.4.1279","volume":"53","author":"T Rohrlack","year":"2008","unstructured":"Rohrlack, T. et al. Oligopeptide chemotypes of the toxic freshwater cyanobacterium Planktothrix can form sub-populations with dissimilar ecological traits. Limnol Oceanogr \n                           53, 1279\u20131293, doi:10.4319\/lo.2008.53.4.1279 (2008).","journal-title":"Limnol Oceanogr"},{"key":"8701_CR33","doi-asserted-by":"publisher","first-page":"3849","DOI":"10.1111\/j.1365-294X.2006.03044.x","volume":"15","author":"R Kurmayer","year":"2006","unstructured":"Kurmayer, R. & Gumpenberger, M. Diversity of microcystin genotypes among populations of the filamentous cyanobacteria Planktothrix rubescens and Planktothrix agardhii. Mol Ecol \n                           15, 3849\u20133861, doi:10.1111\/j.1365-294X.2006.03044.x (2006).","journal-title":"Mol Ecol"},{"key":"8701_CR34","unstructured":"Reynolds, C. S. The ecology of freshwater phytoplankton. (Cambridge Univ. Press, Cambridge and New York, 1984)."},{"key":"8701_CR35","first-page":"153","volume":"7","author":"A Napi\u00f3rkowska-Krzebietke","year":"2007","unstructured":"Napi\u00f3rkowska-Krzebietke, A. et al. Changes in the trophic state of Lake Niegocin after the modernization of a local wastewater treatment plant. Limnol Rev \n                           7, 153\u2013159 (2007).","journal-title":"Limnol Rev"},{"key":"8701_CR36","first-page":"53","volume":"26","author":"I Spodniewska","year":"1978","unstructured":"Spodniewska, I. Phytoplankton as indicator of lake eutrophication. 1. Summer situation in 34 Masurian lakes in 1973. Ekol pol \n                           26, 53\u201370 (1978).","journal-title":"Ekol pol"},{"key":"8701_CR37","first-page":"151","volume":"34","author":"I Spodniewska","year":"1986","unstructured":"Spodniewska, I. Planktonic blue-green algae of lakes in north-eastern Poland. Ekol pol \n                           34, 151\u201383 (1986).","journal-title":"Ekol pol"},{"key":"8701_CR38","unstructured":"Siuda, W., Kali\u0144ski, T., Kauppinen, E. & Chr\u00f3st, R. J. Eutrophication of the southern part of Great Masurian Lakes in 1977\u20132011. Technologia wody \n                           3, 48\u201363 (in Polish, 2014)."},{"key":"8701_CR39","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1111\/j.1462-2920.2004.00626.x","volume":"6","author":"R Kurmayer","year":"2004","unstructured":"Kurmayer, R., Christiansen, G., Fastner, J. & B\u00f6rner, T. Abundance of active and inactive microcystin genotypes in populations of the toxic cyanobacterium Planktothrix spp. Environ Microbiol \n                           6, 831\u2013841, doi:10.1111\/j.1462-2920.2004.00626.x (2004).","journal-title":"Environ Microbiol"},{"key":"8701_CR40","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1038\/ismej.2014.189","volume":"9","author":"R Kurmayer","year":"2015","unstructured":"Kurmayer, R., Blom, J. F., Deng, L. & Pernthaler, J. Integrating phylogeny, geographic niche partitioning and secondary metabolite synthesis in bloom-forming Planktothrix. ISME J \n                           9, 909\u2013921, doi:10.1038\/ismej.2014.189 (2015).","journal-title":"ISME J"},{"key":"8701_CR41","doi-asserted-by":"publisher","first-page":"6803","DOI":"10.1128\/AEM.01455-13","volume":"79","author":"A Tooming-Klunderud","year":"2013","unstructured":"Tooming-Klunderud, A. et al. From green to red: Horizontal gene transfer of the phycoerythrin gene cluster between Planktothrix strains. Appl Environ Microbiol \n                           79, 6803\u20136812, doi:10.1128\/AEM.01455-13 (2013).","journal-title":"Appl Environ Microbiol"},{"key":"8701_CR42","doi-asserted-by":"publisher","unstructured":"Six, C. et al. Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study. Genome Biol R259, doi:10.1186\/gb-2007-8-12-r259 (2007).","DOI":"10.1186\/gb-2007-8-12-r259"},{"key":"8701_CR43","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1111\/j.1574-6941.2010.00990.x","volume":"75","author":"I Jasser","year":"2011","unstructured":"Jasser, I., Kr\u00f3licka, A. & Karnkowska-Ishikawa, A. A novel phylogenetic clade of picocyanobacteria from the Mazurian lakes (Poland) reflects the early ontogeny of glacial lakes. FEMS Microbiol Ecol \n                           75, 89\u201398, doi:10.1111\/j.1574-6941.2010.00990.x (2011).","journal-title":"FEMS Microbiol Ecol"},{"key":"8701_CR44","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1186\/1741-7007-10-100","volume":"10","author":"V Ostermaier","year":"2012","unstructured":"Ostermaier, V., Schanz, F., K\u00f6ster, O. & Kurmayer, R. Stability of toxin gene proportion in red-pigmented populations of the cyanobacterium Planktothrix during 29 years of re-oligotrophication of Lake Z\u00fcrich. BMC Biol \n                           10, 100\u2013115, doi:10.1186\/1741-7007-10-100 (2012).","journal-title":"BMC Biol"},{"key":"8701_CR45","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.1111\/j.1529-8817.2007.00414.x","volume":"43","author":"L Oberhaus","year":"2007","unstructured":"Oberhaus, L., Briand, J. F., Leboulanger, C., Jaquet, S. & Humbert, J. F. Comparative effects of the quality and quantity of light and temperature on the growth of Planktothrix agardhii and P. rubescens. J Phycol \n                           43, 1191\u20131199, doi:10.1111\/j.1529-8817.2007.00414.x (2007).","journal-title":"J Phycol"},{"key":"8701_CR46","doi-asserted-by":"publisher","first-page":"2803","DOI":"10.1016\/j.watres.2010.02.018","volume":"44","author":"W Okello","year":"2010","unstructured":"Okello, W., Ostermaier, V., Portmann, C., Gademann, K. & Kurmayer, R. Spatial isolation favours the divergence in microcystin net production by Microcystis in Ugandan freshwater lakes. Water Res \n                           44, 2803\u20132814, doi:10.1016\/j.watres.2010.02.018 (2010).","journal-title":"Water Res"},{"key":"8701_CR47","doi-asserted-by":"publisher","first-page":"4750","DOI":"10.1128\/AEM.02531-09","volume":"76","author":"M Sabart","year":"2010","unstructured":"Sabart, M. et al. Spatiotemporal variations in microcystin concentrations and in the proportions of microcystin-producing cells in several Microcystis aeruginosa populations. Appl Environ Microbiol \n                           76, 4750\u20134759, doi:10.1128\/AEM.02531-09 (2010).","journal-title":"Appl Environ Microbiol"},{"key":"8701_CR48","doi-asserted-by":"publisher","first-page":"6723","DOI":"10.1128\/AEM.69.11.6723-6730.2003","volume":"69","author":"R Kurmayer","year":"2003","unstructured":"Kurmayer, R. & Kutzenberger, T. Application of real-time PCR for quantification of microcystin genotypes in a population of the toxic cyanobacterium Microcystis sp. Appl Environ Microbiol \n                           69, 6723\u20136730, doi:10.1128\/AEM.69.11.6723\u20136730.2003 (2003).","journal-title":"Appl Environ Microbiol"},{"key":"8701_CR49","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1080\/10635150390235520","volume":"52","author":"S Guindon","year":"2003","unstructured":"Guindon, S. & Gascuel, O. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol \n                           52, 696\u2013704, doi:10.1080\/10635150390235520 (2003).","journal-title":"Syst Biol"},{"key":"8701_CR50","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1080\/10635150600755453","volume":"55","author":"M Anisimova","year":"2006","unstructured":"Anisimova, M. & Gascuel, O. Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Syst Biol \n                           55, 539\u201352, doi:10.1080\/10635150600755453 (2006).","journal-title":"Syst Biol"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08701-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08701-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08701-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T09:07:44Z","timestamp":1671786464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-08701-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,21]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["8701"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-08701-8","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,21]]},"assertion":[{"value":"3 March 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"8342"}}