{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:38:13Z","timestamp":1790645893318,"version":"4.1.0"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T00:00:00Z","timestamp":1503964800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T00:00:00Z","timestamp":1503964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Breast cancer mainly spreads to bone, causing decreased survival of patient. Human antigen R (HuR) and chemokines are important molecules associated with mRNA stability and cell-cell interaction in cancer biology. Here, HuR knockdown inhibited bone metastasis and osteolysis of metastatic breast cancer cells in mice and HuR expression promoted the metastatic ability of cancer cells via CCL20 and GM-CSF. In contrast with the findings for\n                    <jats:italic>GM-CSF<\/jats:italic>\n                    ,\n                    <jats:italic>ELAVL1<\/jats:italic>\n                    and\n                    <jats:italic>CCL20<\/jats:italic>\n                    expressions were markedly increased in breast tumor tissues and\n                    <jats:italic>ELAVL1<\/jats:italic>\n                    expression showed a strong positive correlation with\n                    <jats:italic>CCL20<\/jats:italic>\n                    expression in breast cancer subtypes, particularly the basal-like subtype. Metastasis-free survival and overall survival were decreased in the breast cancer patients with high\n                    <jats:italic>CCL20<\/jats:italic>\n                    expression. We further confirmed the role of CCL20 in breast cancer bone metastasis. Intraperitoneal administration of anti-CCL20 antibodies inhibited osteolytic breast cancer bone metastasis in mice. Treatment with CCL20 noticeably promoted cell invasion and the secretion of MMP-2\/9 in the basal-like triple-negative breast cancer cell lines, not the luminal. Moreover, CCL20 elevated the receptor activator of nuclear factors kappa-B ligand\/osteoprotegerin ratio in breast cancer and osteoblastic cells and mediated the crosstalk between these cells. Collectively, HuR-regulated CCL20 may be an attractive therapeutic target for breast cancer bone metastasis.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-09040-4","type":"journal-article","created":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T05:05:52Z","timestamp":1503378352000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Human antigen R-regulated CCL20 contributes to osteolytic breast cancer bone metastasis"],"prefix":"10.1038","volume":"7","author":[{"given":"Sun Kyoung","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Kyun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun-Jeong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhee","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hwa","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Rim","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8428-9005","authenticated-orcid":false,"given":"Won-Yoon","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,29]]},"reference":[{"key":"9040_CR1","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nrc867","volume":"2","author":"GR Mundy","year":"2002","unstructured":"Mundy, G. R. Metastasis to bone: causes, consequences and therapeutic opportunities. Nat. Rev. Cancer \n                           2, 584\u2013593 (2002).","journal-title":"Nat. Rev. Cancer"},{"key":"9040_CR2","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1056\/NEJMra030831","volume":"350","author":"GD Roodman","year":"2004","unstructured":"Roodman, G. D. Mechanisms of bone metastasis. N. Engl. J. Med. \n                           350, 1655\u20131664 (2004).","journal-title":"N. Engl. J. Med."},{"key":"9040_CR3","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1038\/nm0107-25","volume":"13","author":"GD Roodman","year":"2007","unstructured":"Roodman, G. D. Bone-breaking cancer treatment. Nat. Med. \n                           13, 25\u201326 (2007).","journal-title":"Nat. Med."},{"key":"9040_CR4","doi-asserted-by":"crossref","unstructured":"Bendre, M., Gaddy, D., Nicholas, R. W. & Suva, L. J. Breast cancer metastasis to bone: it is not all about PTHrP. Clin. Orthop. Relat. Res. S39\u2013S45 (2003).","DOI":"10.1097\/01.blo.0000093844.72468.f4"},{"key":"9040_CR5","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.1172\/JCI118947","volume":"98","author":"TA Guise","year":"1996","unstructured":"Guise, T. A. et al. Evidence for a causal role of parathyroid hormone-related protein in the pathogenesis of human breast cancer-mediated osteolysis. J. Clin. Invest. \n                           98, 1544\u20131549 (1996).","journal-title":"J. Clin. Invest."},{"key":"9040_CR6","doi-asserted-by":"publisher","first-page":"3353","DOI":"10.4049\/jimmunol.169.6.3353","volume":"169","author":"P Palmqvist","year":"2002","unstructured":"Palmqvist, P., Persson, E., Conaway, H. H. & Lerner, U. H. IL-6, leukemia inhibitory factor, and oncostatin M stimulate bone resorption and regulate the expression of receptor activator of NF-kappa B ligand, osteoprotegerin, and receptor activator of NF-kappa B in mouse calvariae. J. Immunol. \n                           169, 3353\u20133362 (2002).","journal-title":"J. Immunol."},{"key":"9040_CR7","first-page":"385","volume":"19","author":"J Ferrer Canabate","year":"2002","unstructured":"Ferrer Canabate, J., Tovar, I. & Martinez, P. Osteoprotegrin and RANKL\/RANK system: is it the future of bone metabolism? An. Med. Int. \n                           19, 385\u2013388 (2002).","journal-title":"An. Med. Int."},{"key":"9040_CR8","doi-asserted-by":"crossref","unstructured":"Guise, T. A. & Chirgwin, J. M. Transforming growth factor-beta in osteolytic breast cancer bone metastases. Clin. Orthop. Relat. Res. S32\u2013S38 (2003).","DOI":"10.1097\/01.blo.0000093055.96273.69"},{"key":"9040_CR9","doi-asserted-by":"publisher","first-page":"4238","DOI":"10.1158\/0008-5472.CAN-11-3061","volume":"72","author":"T Hiraga","year":"2012","unstructured":"Hiraga, T. et al. Bone-derived IGF mediates crosstalk between bone and breast cancer cells in bony metastases. Cancer Res. \n                           72, 4238\u20134249 (2012).","journal-title":"Cancer Res."},{"key":"9040_CR10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067533","volume":"8","author":"P Owens","year":"2013","unstructured":"Owens, P. et al. Bone morphogenetic proteins stimulate mammary fibroblasts to promote mammary carcinoma cell invasion. PLOS ONE \n                           8, e67533 (2013).","journal-title":"PLOS ONE"},{"key":"9040_CR11","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1172\/JCI3523","volume":"103","author":"JJ Yin","year":"1999","unstructured":"Yin, J. J. et al. TGF-beta signaling blockade inhibits PTHrP secretion by breast cancer cells and bone metastases development. J. Clin. Invest. \n                           103, 197\u2013206 (1999).","journal-title":"J. Clin. Invest."},{"key":"9040_CR12","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1056\/NEJMoa1105195","volume":"365","author":"RE Coleman","year":"2011","unstructured":"Coleman, R. E. et al. Breast-cancer adjuvant therapy with zoledronic acid. N. Engl. J. Med. \n                           365, 1396\u20131405 (2011).","journal-title":"N. Engl. J. Med."},{"key":"9040_CR13","doi-asserted-by":"publisher","first-page":"2165","DOI":"10.1016\/j.cellsig.2008.05.007","volume":"20","author":"A Doller","year":"2008","unstructured":"Doller, A., Pfeilschifter, J. & Eberhardt, W. Signalling pathways regulating nucleo-cytoplasmic shuttling of the mRNA-binding protein HuR. Cell. Signal. \n                           20, 2165\u20132173 (2008).","journal-title":"Cell. Signal."},{"key":"9040_CR14","doi-asserted-by":"publisher","first-page":"11","DOI":"10.4161\/rna.2.1.1552","volume":"2","author":"I L\u00f3pez de Silanes","year":"2005","unstructured":"L\u00f3pez de Silanes, I., Lal, A. & Gorospe, M. HuR: post-transcriptional paths to malignancy. RNA Biol. \n                           2, 11\u201313 (2005).","journal-title":"RNA Biol."},{"key":"9040_CR15","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1158\/1541-7786.MCR-09-0367","volume":"8","author":"W Kakuguchi","year":"2010","unstructured":"Kakuguchi, W. et al. HuR knockdown changes the oncogenic potential of oral cancer cells. Mol. Cancer Res. \n                           8, 520\u2013528 (2010).","journal-title":"Mol. Cancer Res."},{"key":"9040_CR16","first-page":"2154","volume":"61","author":"LB Nabors","year":"2001","unstructured":"Nabors, L. B., Gillespie, G. Y., Harkins, L. & King, P. H. HuR, a RNA stability factor, is expressed in malignant brain tumors and binds to adenine- and uridine-rich elements within the 3\u2032 untranslated regions of cytokine and angiogenic factor mRNAs. Cancer Res. \n                           61, 2154\u20132161 (2001).","journal-title":"Cancer Res."},{"key":"9040_CR17","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1016\/j.bbrc.2004.07.173","volume":"322","author":"LG Sheflin","year":"2004","unstructured":"Sheflin, L. G., Zou, A. P. & Spaulding, S. W. Androgens regulate the binding of endogenous HuR to the AU-rich 3\u2032UTRs of HIF-1alpha and EGF mRNA. Biochem. Biophys. Res. Commun. \n                           322, 644\u2013651 (2004).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"9040_CR18","doi-asserted-by":"publisher","first-page":"10015","DOI":"10.3390\/ijms140510015","volume":"14","author":"J Wang","year":"2013","unstructured":"Wang, J. et al. Multiple functions of the RNA-Binding protein HuR in cancer progression, treatment responses and prognosis. Int. J. Mol. Sci. \n                           14, 10015\u201310041 (2013).","journal-title":"Int. J. Mol. Sci."},{"key":"9040_CR19","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1159\/000216837","volume":"76","author":"J Wang","year":"2009","unstructured":"Wang, J. et al. The expression of RNA-binding protein HuR in non-small cell lung cancer correlates with vascular endothelial growth factor-C expression and lymph node metastasis. Oncology \n                           76, 420\u2013429 (2009).","journal-title":"Oncology"},{"key":"9040_CR20","doi-asserted-by":"publisher","first-page":"2340","DOI":"10.1093\/emboj\/19.10.2340","volume":"19","author":"W Wang","year":"2000","unstructured":"Wang, W., Caldwell, M. C., Lin, S., Furneaux, H. & Gorospe, M. HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation. EMBO J. \n                           19, 2340\u20132350 (2000).","journal-title":"EMBO J."},{"key":"9040_CR21","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1038\/onc.2008.469","volume":"28","author":"HH Woo","year":"2009","unstructured":"Woo, H. H. et al. Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer. Oncogene \n                           28, 1176\u20131186 (2009).","journal-title":"Oncogene"},{"key":"9040_CR22","doi-asserted-by":"publisher","first-page":"7177","DOI":"10.1128\/MCB.23.20.7177-7188.2003","volume":"23","author":"H Tran","year":"2003","unstructured":"Tran, H., Maurer, F. & Nagamine, Y. Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2. Mol. Cell. Biol. \n                           23, 7177\u20137188 (2003).","journal-title":"Mol. Cell. Biol."},{"key":"9040_CR23","first-page":"1331","volume":"25","author":"Z Yuan","year":"2010","unstructured":"Yuan, Z., Sanders, A. J., Ye, L. & Jiang, W. G. HuR, a key post-transcriptional regulator, and its implication in progression of breast cancer. Histol. Histopathol. \n                           25, 1331\u20131340 (2010).","journal-title":"Histol. Histopathol."},{"key":"9040_CR24","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1093\/carcin\/bgq052","volume":"31","author":"S Danilin","year":"2010","unstructured":"Danilin, S. et al. Role of the RNA-binding protein HuR in human renal cell carcinoma. Carcinogenesis \n                           31, 1018\u20131026 (2010).","journal-title":"Carcinogenesis"},{"key":"9040_CR25","first-page":"1205","volume":"28","author":"L Lauriola","year":"2013","unstructured":"Lauriola, L. et al. Hu\/elav RNA-binding protein HuR regulates parathyroid hormone related peptide expression in human lung adenocarcinoma cells. Histol. Histopathol. \n                           28, 1205\u20131216 (2013).","journal-title":"Histol. Histopathol."},{"issue":"Suppl.","key":"9040_CR26","first-page":"S82","volume":"123","author":"M Locati","year":"2005","unstructured":"Locati, M., Bonecchi, R. & Corsi, M. M. Chemokines and their receptors: roles in specific clinical conditions and measurement in the clinical laboratory. Am. J. Clin. Pathol. \n                           123(Suppl.), S82\u2013S95 (2005).","journal-title":"Am. J. Clin. Pathol."},{"key":"9040_CR27","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1038\/nrc1388","volume":"4","author":"F Balkwill","year":"2004","unstructured":"Balkwill, F. Cancer and the chemokine network. Nat. Rev. Cancer \n                           4, 540\u2013550 (2004).","journal-title":"Nat. Rev. Cancer"},{"key":"9040_CR28","doi-asserted-by":"publisher","DOI":"10.1186\/bcr3016","volume":"13","author":"WL Jamieson-Gladney","year":"2011","unstructured":"Jamieson-Gladney, W. L., Zhang, Y., Fong, A. M., Meucci, O. & Fatatis, A. The chemokine receptor CX3CR1 is directly involved in the arrest of breast cancer cells to the skeleton. Breast Cancer Res. \n                           13, R91 (2011).","journal-title":"Breast Cancer Res."},{"key":"9040_CR29","doi-asserted-by":"publisher","first-page":"2221","DOI":"10.1172\/JCI59535","volume":"122","author":"D Liu","year":"2012","unstructured":"Liu, D. et al. IL-15 protects NKT cells from inhibition by tumor-associated macrophages and enhances antimetastatic activity. J. Clin. Invest. \n                           122, 2221\u20132233 (2012).","journal-title":"J. Clin. Invest."},{"key":"9040_CR30","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.canlet.2007.05.013","volume":"256","author":"D Raman","year":"2007","unstructured":"Raman, D., Baugher, P. J., Thu, Y. M. & Richmond, A. Role of chemokines in tumor growth. Cancer Lett. \n                           256, 137\u2013165 (2007).","journal-title":"Cancer Lett."},{"key":"9040_CR31","doi-asserted-by":"publisher","first-page":"1652","DOI":"10.1111\/j.1349-7006.2007.00606.x","volume":"98","author":"K Koizumi","year":"2007","unstructured":"Koizumi, K., Hojo, S., Akashi, T., Yasumoto, K. & Saiki, I. Chemokine receptors in cancer metastasis and cancer cell-derived chemokines in host immune response. Cancer Sci. \n                           98, 1652\u20131658 (2007).","journal-title":"Cancer Sci."},{"key":"9040_CR32","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1007\/s10555-006-9019-x","volume":"25","author":"J Wang","year":"2006","unstructured":"Wang, J., Loberg, R. & Taichman, R. S. The pivotal role of CXCL12 (SDF-1)\/CXCR4 axis in bone metastasis. Cancer Metastasis Rev. \n                           25, 573\u2013587 (2006).","journal-title":"Cancer Metastasis Rev."},{"key":"9040_CR33","doi-asserted-by":"publisher","first-page":"e179","DOI":"10.5301\/JBM.2012.9345","volume":"27","author":"JL Steiner","year":"2012","unstructured":"Steiner, J. L. & Murphy, E. A. Importance of chemokine (CC-motif) ligand 2 in breast cancer. Int. J. Biol. Markers \n                           27, e179\u2013e185 (2012).","journal-title":"Int. J. Biol. Markers"},{"key":"9040_CR34","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/j.bone.2014.01.015","volume":"61","author":"A Kamalakar","year":"2014","unstructured":"Kamalakar, A. et al. Circulating interleukin-8 levels explain breast cancer osteolysis in mice and humans. Bone \n                           61, 176\u2013185 (2014).","journal-title":"Bone"},{"key":"9040_CR35","doi-asserted-by":"crossref","unstructured":"Campbell, J. P., Merkel, A. R., Masood-Campbell, S. K., Elefteriou, F. & Sterling, J. A. Models of bone metastasis. J. Vis. Exp. JoVE e4260 (2012).","DOI":"10.3791\/4260"},{"key":"9040_CR36","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1038\/nrd2372","volume":"11","author":"BL Eckhardt","year":"2012","unstructured":"Eckhardt, B. L., Francis, P. A., Parker, B. S. & Anderson, R. L. Strategies for the discovery and development of therapies for metastatic breast cancer. Nat. Rev. Drug Discov. \n                           11, 479\u2013497 (2012).","journal-title":"Nat. Rev. Drug Discov."},{"key":"9040_CR37","doi-asserted-by":"publisher","first-page":"4835","DOI":"10.1128\/MCB.24.11.4835-4847.2004","volume":"24","author":"R Winzen","year":"2004","unstructured":"Winzen, R. et al. Distinct domains of AU-rich elements exert different functions in mRNA destabilization and stabilization by p38 mitogen-activated protein kinase or HuR. Mol. Cell. Biol. \n                           24, 4835\u20134847 (2004).","journal-title":"Mol. Cell. Biol."},{"key":"9040_CR38","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1038\/nm1519","volume":"13","author":"BK Park","year":"2007","unstructured":"Park, B. K. et al. NF-kappaB in breast cancer cells promotes osteolytic bone metastasis by inducing osteoclastogenesis via GM-CSF. Nat. Med. \n                           13, 62\u201369 (2007).","journal-title":"Nat. Med."},{"key":"9040_CR39","doi-asserted-by":"publisher","first-page":"6150","DOI":"10.1158\/0008-5472.CAN-09-2758","volume":"70","author":"N Rucci","year":"2010","unstructured":"Rucci, N. et al. Receptor activator of NF-kappaB ligand enhances breast cancer-induced osteolytic lesions through upregulation of extracellular matrix metalloproteinase inducer\/CD147. Cancer Res. \n                           70, 6150\u20136160 (2010).","journal-title":"Cancer Res."},{"key":"9040_CR40","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/j.bbrc.2011.04.035","volume":"408","author":"ZN Tang","year":"2011","unstructured":"Tang, Z. N., Zhang, F., Tang, P., Qi, X. W. & Jiang, J. Hypoxia induces RANK and RANKL expression by activating HIF-1alpha in breast cancer cells. Biochem. Biophys. Res. Commun. \n                           408, 411\u2013416 (2011).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"9040_CR41","doi-asserted-by":"publisher","DOI":"10.1186\/bcr2781","volume":"12","author":"YC Chen","year":"2010","unstructured":"Chen, Y. C., Sosnoski, D. M. & Mastro, A. M. Breast cancer metastasis to the bone: mechanisms of bone loss. Breast Cancer Res. \n                           12, 215 (2010).","journal-title":"Breast Cancer Res."},{"key":"9040_CR42","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1097\/01.pgp.0000236946.82334.07","volume":"26","author":"SJ Lim","year":"2007","unstructured":"Lim, S. J., Kim, H. J., Kim, J. Y., Park, K. & Lee, C. M. Expression of HuR is associated with increased cyclooxygenase-2 expression in uterine cervical carcinoma. Int. J. Gynecol. Pathol. \n                           26, 229\u2013234 (2007).","journal-title":"Int. J. Gynecol. Pathol."},{"key":"9040_CR43","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1177\/107327481201900203","volume":"19","author":"RL Theriault","year":"2012","unstructured":"Theriault, R. L. & Theriault, R. L. Biology of bone metastases. Cancer Contr. J. Moffitt Cancer Center \n                           19, 92\u2013101 (2012).","journal-title":"Cancer Contr. J. Moffitt Cancer Center"},{"key":"9040_CR44","doi-asserted-by":"publisher","first-page":"2133","DOI":"10.1158\/0008-5472.CAN-08-1405","volume":"69","author":"TD Eubank","year":"2009","unstructured":"Eubank, T. D. et al. Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages. Cancer Res. \n                           69, 2133\u20132140 (2009).","journal-title":"Cancer Res."},{"key":"9040_CR45","doi-asserted-by":"publisher","first-page":"5014","DOI":"10.1158\/0008-5472.CAN-10-0100","volume":"70","author":"J Dai","year":"2010","unstructured":"Dai, J. et al. Reversal of chemotherapy-induced leukopenia using granulocyte macrophage colony-stimulating factor promotes bone metastasis that can be blocked with osteoclast inhibitors. Cancer Res. \n                           70, 5014\u20135023 (2010).","journal-title":"Cancer Res."},{"key":"9040_CR46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024671","volume":"6","author":"KJ Chen","year":"2011","unstructured":"Chen, K. J. et al. Selective recruitment of regulatory T cell through CCR6-CCL20 in hepatocellular carcinoma fosters tumor progression and predicts poor prognosis. PLOS ONE \n                           6, e24671 (2011).","journal-title":"PLOS ONE"},{"key":"9040_CR47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019495","volume":"6","author":"J Liu","year":"2011","unstructured":"Liu, J. et al. Tumor-associated macrophages recruit CCR6+ regulatory T cells and promote the development of colorectal cancer via enhancing CCL20 production in mice. PLOS ONE \n                           6, e19495 (2011).","journal-title":"PLOS ONE"},{"key":"9040_CR48","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1038\/bjc.2015.290","volume":"113","author":"JY Liu","year":"2015","unstructured":"Liu, J. Y. et al. CTL- vs Treg lymphocyte-attracting chemokines, CCL4 and CCL20, are strong reciprocal predictive markers for survival of patients with oesophageal squamous cell carcinoma. Br. J. Cancer \n                           113, 747\u2013755 (2015).","journal-title":"Br. J. Cancer"},{"key":"9040_CR49","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.biopha.2014.12.008","volume":"69","author":"CY Zhang","year":"2015","unstructured":"Zhang, C. Y. et al. The role of CCL20\/CCR6 axis in recruiting Treg cells to tumor sites of NSCLC patients. Biomed. Pharmacother. \n                           69, 242\u2013248 (2015).","journal-title":"Biomed. Pharmacother."},{"key":"9040_CR50","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1159\/000092777","volume":"27","author":"C Rubie","year":"2006","unstructured":"Rubie, C. et al. Involvement of chemokine receptor CCR6 in colorectal cancer metastasis. Tumour Biol. \n                           27, 166\u2013174 (2006).","journal-title":"Tumour Biol."},{"key":"9040_CR51","doi-asserted-by":"publisher","first-page":"6840","DOI":"10.1158\/0008-5472.CAN-08-0402","volume":"68","author":"N Giuliani","year":"2008","unstructured":"Giuliani, N. et al. CC-chemokine ligand 20\/macrophage inflammatory protein-3alpha and CC-chemokine receptor 6 are overexpressed in myeloma microenvironment related to osteolytic bone lesions. Cancer Res. \n                           68, 6840\u20136850 (2008).","journal-title":"Cancer Res."},{"key":"9040_CR52","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/nature01658","volume":"423","author":"WJ Boyle","year":"2003","unstructured":"Boyle, W. J., Simonet, W. S. & Lacey, D. L. Osteoclast differentiation and activation. Nature \n                           423, 337\u2013342 (2003).","journal-title":"Nature"},{"key":"9040_CR53","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/nature01660","volume":"423","author":"S Harada","year":"2003","unstructured":"Harada, S. & Rodan, G. A. Control of osteoblast function and regulation of bone mass. Nature \n                           423, 349\u2013355 (2003).","journal-title":"Nature"},{"key":"9040_CR54","doi-asserted-by":"crossref","first-page":"395","DOI":"10.3892\/ol.2011.487","volume":"3","author":"L Zhang","year":"2012","unstructured":"Zhang, L. et al. C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway. Oncol. Lett. \n                           3, 395\u2013400 (2012).","journal-title":"Oncol. Lett."},{"key":"9040_CR55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0122764","volume":"10","author":"KR Kim","year":"2015","unstructured":"Kim, K. R. et al. 15-deoxy-\u03b412,14-prostaglandin j2 inhibits osteolytic breast cancer bone metastasis and estrogen deficiency-induced bone loss. PLOS ONE \n                           10, e0122764 (2015).","journal-title":"PLOS ONE"},{"key":"9040_CR56","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1002\/path.4597","volume":"237","author":"HJ Kim","year":"2015","unstructured":"Kim, H. J. et al. Loss of RUNX3 expression promotes cancer-associated bone destruction by regulating CCL5, CCL19 and CXCL11 in non-small cell lung cancer. J. Pathol. \n                           237, 520\u2013531 (2015).","journal-title":"J. Pathol."},{"key":"9040_CR57","volume":"67","author":"JP Campbell","year":"2012","unstructured":"Campbell, J. P., Merkel, A. R., Masood-Campbell, S. K., Elefteriou, F. & Sterling, J. A. Models of bone metastasis. J. Vis. Exp. \n                           67, e4260 (2012).","journal-title":"J. Vis. Exp."},{"key":"9040_CR58","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1007\/s10549-009-0674-9","volume":"123","author":"B Gy\u00f6rffy","year":"2010","unstructured":"Gy\u00f6rffy, B. et al. An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res. Treat. \n                           123, 725\u2013731 (2010).","journal-title":"Breast Cancer Res. Treat."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09040-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09040-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09040-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T04:28:16Z","timestamp":1671769696000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09040-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,29]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["9040"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-09040-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,29]]},"assertion":[{"value":"11 April 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"9610"}}