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Parallel factor (PARAFAC) modelling of the sample fluorescence spectra identified humic-like and protein-like or tyrosine-like components in both untreated and irradiated EPS samples. After 58\u2009hours of solar irradiation, humic-like substances were entirely decomposed, while the regenerated protein-like substance from EPS was the key component in the irradiated samples. Degradation and reformation of EPS occurred which was confirmed by the results of size exclusion chromatography, dissolved organic carbon, total protein and total polysaccharide analyses. Irradiated EPS was composed of \u2013COOH or C\u2009=\u2009O (amide I band) and \u2013NH and \u2013CN (amide II band), while Fourier transform infrared spectroscopy (FTIR) of TEP revealed more acidic \u2013COOH and \u2013C\u2013O groups, indicating typical acidic protein-like TEP. The regenerated protein-like substances could form complexes with free metals originating from degraded EPS in irradiated samples, which could be responsible for the formation of TEP\/floc in the aqueous media. These results suggest that TEP\/floc formation from EPS could occur by a complexation mechanism between dissolved organic matter and metals, thereby causing ionic charge neutralisation upon sunlight exposure.<\/jats:p>","DOI":"10.1038\/s41598-017-09066-8","type":"journal-article","created":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T07:19:04Z","timestamp":1502867944000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences"],"prefix":"10.1038","volume":"7","author":[{"given":"Mashura","family":"Shammi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangliang","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khan M. 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