{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T02:24:47Z","timestamp":1768616687312,"version":"3.49.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T00:00:00Z","timestamp":1503273600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T00:00:00Z","timestamp":1503273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Neosartorya fumigata<\/jats:italic> (<jats:italic>Aspergillus fumigatus<\/jats:italic>) is the most common cause of invasive aspergillosis, a frequently fatal lung disease primarily affecting immunocompromised individuals. This opportunistic fungal pathogen produces several classes of specialised metabolites including products of a branch of the ergot alkaloid pathway called fumigaclavines. The biosynthesis of the <jats:italic>N. fumigata<\/jats:italic> ergot alkaloids and their relation to those produced by alternate pathway branches in fungi from the plant-inhabiting Clavicipitaceae have been well-characterised, but the potential role of these alkaloids in animal pathogenesis has not been studied extensively. We investigated the contribution of ergot alkaloids to virulence of <jats:italic>N. fumigata<\/jats:italic> by measuring mortality in the model insect <jats:italic>Galleria mellonella<\/jats:italic>. Larvae were injected with conidia (asexual spores) of two different wild-type strains of <jats:italic>N. fumigata<\/jats:italic> and three different ergot alkaloid mutants derived by previous gene knockouts and differing in ergot alkaloid profiles. Elimination of all ergot alkaloids significantly reduced virulence of <jats:italic>N. fumigata<\/jats:italic> in <jats:italic>G. mellonella<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). Mutants accumulating intermediates but not the pathway end product fumigaclavine C also were less virulent than the wild type (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0003). The data indicate that ergot alkaloids contribute to virulence of <jats:italic>N. fumigata<\/jats:italic> in this insect model and that fumigaclavine C is important for full virulence.<\/jats:p>","DOI":"10.1038\/s41598-017-09107-2","type":"journal-article","created":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T10:46:57Z","timestamp":1502794017000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4159-164X","authenticated-orcid":false,"given":"Daniel G.","family":"Panaccione","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie L.","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,8,21]]},"reference":[{"key":"9107_CR1","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1039\/C0NP00060D","volume":"28","author":"C Wallwey","year":"2011","unstructured":"Wallwey, C. & Li, S.-M. 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