{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:07:38Z","timestamp":1787508458916,"version":"build-2736575974"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T00:00:00Z","timestamp":1503360000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T00:00:00Z","timestamp":1503360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The echinocandins are relatively new antifungal drugs that represent, together with the older azoles, the recommended and\/or preferred agents to treat candidaemia and other forms of invasive candidiasis in human patients. If \u201ctime is of the essence\u201d to reduce the mortality for these infections, the administration of appropriate antifungal therapy could be accelerated by the timely reporting of laboratory test results. In this study, we attempted to validate a MALDI-ToF mass spectrometry-based assay for the\n                    <jats:underline>a<\/jats:underline>\n                    nti\n                    <jats:underline>f<\/jats:underline>\n                    ungal\n                    <jats:underline>s<\/jats:underline>\n                    usceptibility\n                    <jats:underline>t<\/jats:underline>\n                    esting (AFST) of the potentially multidrug-resistant pathogen\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    against anidulafungin and fluconazole. The practical applicability of the assay, reported here as MS-AFST, was assessed with a panel of clinical isolates that were selected to represent phenotypically and genotypically\/molecularly susceptible or resistant strains. The data show the potential of our assay for rapid detection of antifungal resistance, although the MS-AFST assay performed at 3\u2009h of the\n                    <jats:italic>in vitro<\/jats:italic>\n                    antifungal exposure failed to detect\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    isolates with echinocandin\u00a0resistance-associated\n                    <jats:italic>FKS2<\/jats:italic>\n                    mutations. However, cell growth kinetics in the presence of anidulafungin revealed important cues about the\n                    <jats:italic>in vitro<\/jats:italic>\n                    fitness of\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    isolates, which may lead to genotypic or phenotypic antifungal testing in clinical practice.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-09329-4","type":"journal-article","created":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T09:34:00Z","timestamp":1502876040000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Potential Use of MALDI-ToF Mass Spectrometry for Rapid Detection of Antifungal Resistance in the Human Pathogen Candida glabrata"],"prefix":"10.1038","volume":"7","author":[{"given":"Antonietta","family":"Vella","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"De Carolis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrica","family":"Mello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Perlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5244-4178","authenticated-orcid":false,"given":"Dominique","family":"Sanglard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9780-7059","authenticated-orcid":false,"given":"Maurizio","family":"Sanguinetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brunella","family":"Posteraro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,22]]},"reference":[{"key":"9329_CR1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404","volume":"4","author":"GD Brown","year":"2012","unstructured":"Brown, G. 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Mycoses \n                           60, 11\u201319, doi:10.1111\/myc.12535 (2017).","journal-title":"Mycoses"},{"issue":"Suppl 6","key":"9329_CR7","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1111\/1469-0691.12539","volume":"20","author":"J Guinea","year":"2014","unstructured":"Guinea, J. Global trends in the distribution of Candida species causing candidemia. Clinical Microbiology and Infection \n                           20(Suppl 6), 5\u201310, doi:10.1111\/1469-0691.12539 (2014).","journal-title":"Clinical Microbiology and Infection"},{"key":"9329_CR8","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.1128\/AAC.01138-09","volume":"54","author":"F Chapeland-Leclerc","year":"2010","unstructured":"Chapeland-Leclerc, F. et al. Acquisition of flucytosine, azole, and caspofungin resistance in Candida glabrata bloodstream isolates serially obtained from a hematopoietic stem cell transplant recipient. 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American Journal of Medicine \n                           125(Suppl 1), S3\u201313, doi:10.1016\/j.amjmed.2011.11.001 (2012).","journal-title":"American Journal of Medicine"},{"key":"9329_CR15","doi-asserted-by":"publisher","first-page":"6573","DOI":"10.1128\/AAC.01574-16","volume":"60","author":"Y Zhao","year":"2016","unstructured":"Zhao, Y. et al. Rapid detection of FKS-associated echinocandin resistance in Candida glabrata. Antimicrobial Agents and Chemotherapy \n                           60, 6573\u20136577, doi:10.1128\/AAC.01574-16 (2016).","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"9329_CR16","doi-asserted-by":"publisher","first-page":"947","DOI":"10.2217\/fmb.14.55","volume":"9","author":"B Posteraro","year":"2014","unstructured":"Posteraro, B. & Sanguinetti, M. The future of fungal susceptibility testing. 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M27-S4 Reference method for broth dilution antifungal susceptibility testing of yeasts; fourth informational supplement CLSI document MM18-A (Clinical and Laboratory Standards Institute, 2012)."},{"key":"9329_CR28","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofv163","volume":"2","author":"S Vallabhaneni","year":"2015","unstructured":"Vallabhaneni, S. et al. Epidemiology and risk factors for echinocandin nonsusceptible Candida glabrata bloodstream infections: data from a large multisite population-based candidemia surveillance program, 2008\u20132014. Open Forum Infectious Diseases \n                           2, ofv163, doi:10.1093\/ofid\/ofv163 (2015).","journal-title":"Open Forum Infectious Diseases"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09329-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09329-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09329-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T04:19:37Z","timestamp":1671769177000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-09329-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,22]]},"references-count":28,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["9329"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-09329-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,22]]},"assertion":[{"value":"21 February 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 July 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"9099"}}