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By temperature triggered sol-gel phase transition of copolymers, EGCG-loaded GN was prepared at 32\u2009\u00b0C and characterized by FTIR, NMR, and HPLC analyses. Results of WST-1 and live\/dead assays showed that GN materials have good compatibility with corneal epithelial cells. Gradual biodegradation of delivery carriers allowed sustained release of EGCG without drug toxicity. Anti-inflammatory and antioxidant activity studies also indicated effective therapeutic drug levels at each time point within 3 days of release. In a rabbit dry eye model, corneal epithelial defects was ameliorated by treatment with single-dose administration of EGCG-containing GN. Furthermore, drug molecules released from carrier materials could prevent further tear evaporation and loss of mucin-secreting goblet cells in diseased animals. Our findings suggest that GN carrier is responsible for enhanced pharmacological efficacy of topically instilled EGCG, thereby demonstrating the benefits of using biodegradable\n                    <jats:italic>in situ<\/jats:italic>\n                    gelling delivery system to overcome the drawbacks of limited dry eye relief associated with eye drop dosage form.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-09913-8","type":"journal-article","created":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T10:37:42Z","timestamp":1503052662000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Epigallocatechin Gallate-Loaded Gelatin-g-Poly(N-Isopropylacrylamide) as a New Ophthalmic Pharmaceutical Formulation for Topical Use in the Treatment of Dry Eye Syndrome"],"prefix":"10.1038","volume":"7","author":[{"given":"Li-Jyuan","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9227-8549","authenticated-orcid":false,"given":"Jui-Yang","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,24]]},"reference":[{"key":"9913_CR1","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1111\/j.1463-5224.2010.00834.x","volume":"14","author":"MW Shafaa","year":"2011","unstructured":"Shafaa, M. 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