{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:29:14Z","timestamp":1790036954200,"version":"4.0.1"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We compared the performance of the Roche Diagnostics Elecsys immunoassay for the detection of\n                    <jats:italic>Treponema pallidum<\/jats:italic>\n                    specific antibodies in patient serum samples with that of the Abbott Laboratories Architect chemiluminescent microparticle immunoassay and the InTec and KHB enzyme-linked immunosorbent assays, which are commonly used in China. We tested 13,767 serum samples collected from 13 independent laboratories throughout China, which included samples from 999 previously confirmed syphilis cases and 158 \u2018borderline\u2019 samples previously identified using the Architect, InTec, and KHB tests. The Mikrogen Syphilis Immunoblot was used to confirm positive test results. The consistency between the four different assays was 100%. The sensitivity of Elecsys immunoassay was 100% versus 98.26% for Architect, 99.11% for InTec; and 98.56% for KHB. The specificity of the Elecsys immunoassay was 99.81% versus 99.74% for Architect; 99.93% versus 99.80% for InTec; and 99.85% versus 99.77% for KHB. For borderline samples, the Elecsys immunoassay yielded no false-negative results and fewer false-positive results, compared to the other tests. Considering the ease-of-use, automation, high speed, and high throughput capacity of the Elecsys assay, the higher sensitivity and specificity indicate it is superior for routine screening of serum samples for syphilis diagnosis.\n                  <\/jats:p>","DOI":"10.1038\/s41598-017-10103-9","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T11:53:51Z","timestamp":1503316431000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of the Elecsys syphilis immunoassay for routine screening of serum samples in China"],"prefix":"10.1038","volume":"7","author":[{"given":"Chuanmin","family":"Tao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoke","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyang","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingchang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qishui","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianzhang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanlan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,25]]},"reference":[{"key":"10103_CR1","doi-asserted-by":"crossref","unstructured":"Organization, W. H. One stone to kill two birds. An interview with Xiang-Sheng Chen. World Health Organization, Geneva, Switzerland, http:\/\/www.who.int\/bulletin\/volumes\/87\/11\/09-041109\/en\/ (2009).","DOI":"10.2471\/BLT.09.041109"},{"key":"10103_CR2","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/S0140-6736(07)60074-9","volume":"369","author":"ZQ Chen","year":"2007","unstructured":"Chen, Z. Q. et al. Syphilis in China: results of a national surveillance programme. Lancet \n                           369, 132\u2013138 (2007).","journal-title":"Lancet"},{"key":"10103_CR3","doi-asserted-by":"publisher","first-page":"452","DOI":"10.2471\/BLT.09.070326","volume":"88","author":"JD Tucker","year":"2010","unstructured":"Tucker, J. D. et al. Scaling up syphilis testing in China: implementation beyond the clinic. Bull World Health Organ \n                           88, 452\u2013457 (2010).","journal-title":"Bull World Health Organ"},{"key":"10103_CR4","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1072-z","volume":"15","author":"W Zhang","year":"2015","unstructured":"Zhang, W. et al. Syphilis in the economic center of South China: results from a real-time, web-based surveillance program. BMC Infect Dis \n                           15, 318 (2015).","journal-title":"BMC Infect Dis"},{"key":"10103_CR5","unstructured":"Prevention., C. C. f. D. C. a. Annual infectious disease report. Center for Disease Control and Prevention, China, http:\/\/www.nhfpc.gov.cn\/jkj\/s3578\/201502\/847c041a3bac4c3e844f17309be0cabd.shtml (2015)."},{"key":"10103_CR6","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1128\/CMR.19.1.29-49.2006","volume":"19","author":"RE Lafond","year":"2006","unstructured":"Lafond, R. E. & Lukehart, S. A. Biological basis for syphilis. Clin Microbiol Rev \n                           19, 29\u201349 (2006).","journal-title":"Clin Microbiol Rev"},{"key":"10103_CR7","first-page":"433","volume":"86","author":"PL Mattei","year":"2012","unstructured":"Mattei, P. L., Beachkofsky, T. M., Gilson, R. T. & Wisco, O. J. Syphilis: a reemerging infection. Am Fam Physician \n                           86, 433\u2013440 (2012).","journal-title":"Am Fam Physician"},{"key":"10103_CR8","doi-asserted-by":"publisher","first-page":"1581","DOI":"10.1111\/jdv.12734","volume":"28","author":"M Janier","year":"2014","unstructured":"Janier, M. et al. 2014 European guideline on the management of syphilis. J Eur Acad Dermatol Venereol \n                           28, 1581\u20131593 (2014).","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"10103_CR9","unstructured":"Organization, W. H. Screening donated blood for transfusion-transmissible infections. World Health Organization, Geneva, Switzerland, http:\/\/www.who.int\/bloodsafety\/ScreeningDonatedBloodforTransfusion.pdf (2010)."},{"key":"10103_CR10","unstructured":"Prevention, C. f. D. C. A. Sexually transmitted diseases treatment guidelines. 2015, 1\u2013137 (2015)."},{"key":"10103_CR11","unstructured":"Administration, F. a. D. Guidance for Industry: Recommendations for screening, testing, and management of blood donors and blood components based on screening tests for syphilis. Food and Drug Administration, USA http:\/\/www.fda.gov\/downloads\/BiologicsBloodVaccines\/GuidanceComplianceRegulatoryInformation\/Guidances\/UCM340993.pdf (2014)."},{"key":"10103_CR12","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1097\/OLQ.0b013e31819db594","volume":"36","author":"LN Wang","year":"2009","unstructured":"Wang, L. N. & Li, J. M. Evaluation of immunoglobulin M and G Western blot and ELISA for screening antibodies to Treponema pallidum in blood donors. Sex Transm Dis \n                           36, 413\u2013416 (2009).","journal-title":"Sex Transm Dis"},{"key":"10103_CR13","first-page":"1747","volume":"15","author":"QL Zhang","year":"2015","unstructured":"Zhang, Q. L., Zhou, J. & Guo, Y. Q. Kappa consistency analysis between Kehua TP-ELISA and Murex TP-ELISA. Shanxi Medical Journal \n                           15, 1747\u20131749 (2015).","journal-title":"Shanxi Medical Journal"},{"key":"10103_CR14","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1086\/655832","volume":"51","author":"AC Sena","year":"2010","unstructured":"Sena, A. C., White, B. L. & Sparling, P. F. Novel Treponema pallidum serologic tests: a paradigm shift in syphilis screening for the 21st century. Clin Infect Dis \n                           51, 700\u2013708 (2010).","journal-title":"Clin Infect Dis"},{"key":"10103_CR15","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1136\/sti.2008.031872","volume":"85","author":"H Young","year":"2009","unstructured":"Young, H., Pryde, J., Duncan, L. & Dave, J. The Architect Syphilis assay for antibodies to Treponema pallidum: an automated screening assay with high sensitivity in primary syphilis. Sex Transm Infect \n                           85, 19\u201323 (2009).","journal-title":"Sex Transm Infect"},{"key":"10103_CR16","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1128\/CVI.00505-14","volume":"22","author":"M Enders","year":"2015","unstructured":"Enders, M. et al. Performance evaluation of the Elecsys syphilis assay for the detection of total antibodies to Treponema pallidum. Clin Vaccine Immunol \n                           22, 17\u201326 (2015).","journal-title":"Clin Vaccine Immunol"},{"key":"10103_CR17","doi-asserted-by":"publisher","first-page":"4584","DOI":"10.1172\/JCI57173","volume":"121","author":"EL Ho","year":"2011","unstructured":"Ho, E. L. & Lukehart, S. A. Syphilis: using modern approaches to understand an old disease. J Clin Invest \n                           121, 4584\u20134592 (2011).","journal-title":"J Clin Invest"},{"key":"10103_CR18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/CMR.8.1.1","volume":"8","author":"SA Larsen","year":"1995","unstructured":"Larsen, S. A., Steiner, B. M. & Rudolph, A. H. Laboratory diagnosis and interpretation of tests for syphilis. Clin Microbiol Rev \n                           8, 1\u201321 (1995).","journal-title":"Clin Microbiol Rev"},{"issue":"Suppl 1","key":"10103_CR19","doi-asserted-by":"publisher","first-page":"S75","DOI":"10.1002\/ajh.23196","volume":"87","author":"TL Ortel","year":"2012","unstructured":"Ortel, T. L. Antiphospholipid syndrome: laboratory testing and diagnostic strategies. Am J Hematol \n                           87(Suppl 1), S75\u201381 (2012).","journal-title":"Am J Hematol"},{"key":"10103_CR20","first-page":"413","volume":"68","author":"JJ van der Sluis","year":"1992","unstructured":"van der Sluis, J. J. Laboratory techniques in the diagnosis of syphilis: a review. Genitourin Med \n                           68, 413\u2013419 (1992).","journal-title":"Genitourin Med"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10103-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10103-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10103-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T04:37:07Z","timestamp":1671770227000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10103-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,25]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["10103"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-10103-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,25]]},"assertion":[{"value":"8 February 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 August 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"C.T., X.H., J.Z., S.P., Z.T., Y.Q., Q.O., X.H., and L.W. have previously received fees from Roche Diagnostics for professional speaking engagements that were not related to this study. The remaining authors have no conflicts of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"9559"}}