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ATP-binding cassette (ABC) transporters are known to play a crucial role in the resistance to multiple antibiotics. In this study, the potential contribution of an ABC transporter in <jats:italic>C. difficile<\/jats:italic> multidrug resistance was investigated. The expression level of the <jats:italic>cd2068<\/jats:italic> gene in <jats:italic>C. difficile<\/jats:italic> encoding an ABC transporter was up-regulated following the exposure to certain antibiotics compared to the control cells. Heterologous expression of CD2068 in <jats:italic>Escherichia coli<\/jats:italic> revealed that it mediated the efflux of fluorescent substrates and conferred resistance to multiple drugs. The CD2068-associated ATPase activity in membrane vesicles was also stimulated by various antibiotics. Furthermore, the insertional inactivation of the <jats:italic>cd2068<\/jats:italic> gene in <jats:italic>C. difficile<\/jats:italic> led to a significant increase in susceptibility to antibiotics, which could be genetically complemented, supporting that CD2068 was directly associated to the drug resistance. These results demonstrate the potential role for the ABC transporter CD2068 in the resistance mechanism against multiple drugs in <jats:italic>C. difficile<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-017-10155-x","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T10:32:49Z","timestamp":1503570769000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["CD2068 potentially mediates multidrug efflux in Clostridium difficile"],"prefix":"10.1038","volume":"7","author":[{"given":"Chawalit","family":"Ngernsombat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suthasinee","family":"Sreesai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phurt","family":"Harnvoravongchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surang","family":"Chankhamhaengdecha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6376-5575","authenticated-orcid":false,"given":"Tavan","family":"Janvilisri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,30]]},"reference":[{"issue":"suppl 1","key":"10155_CR1","doi-asserted-by":"publisher","first-page":"S12","DOI":"10.1086\/521863","volume":"46","author":"JG Bartlett","year":"2008","unstructured":"Bartlett, J. 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