{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:14:42Z","timestamp":1790734482644,"version":"4.1.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Legionella pneumophila<\/jats:italic> infects human alveolar macrophages and is responsible for Legionnaire\u2019s disease, a severe form of pneumonia. <jats:italic>L. pneumophila<\/jats:italic> encodes more than 300 putative effectors, which are translocated into the host cell via the Dot\/Icm type IV secretion system. These effectors highjack the host\u2019s cellular processes to allow bacterial intracellular growth and replication. Here we adopted a multidisciplinary approach to investigate WipB, a Dot\/Icm effector of unknown function. The crystal structure of the N-terminal domain at 1.7\u2009\u00c5 resolution comprising residues 25 to 344 revealed that WipB harbours a Ser\/Thr phosphatase domain related to the eukaryotic phospho-protein phosphatase (PPP) family. The C-terminal domain (residues 365\u2013524) is sufficient to pilot the effector to acidified LAMP1-positive lysosomal compartments, where WipB interacts with the v-ATPase and the associated LAMTOR1 phosphoprotein, key components of the lysosomal nutrient sensing (LYNUS) apparatus that controls the mammalian target of rapamycin (mTORC1) kinase complex at the lysosomal surface. We propose that WipB is a lysosome-targeted phosphatase that modulates cellular nutrient sensing and the control of energy metabolism during <jats:italic>Legionella<\/jats:italic> infection.<\/jats:p>","DOI":"10.1038\/s41598-017-10249-6","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T10:39:30Z","timestamp":1503311970000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The Legionella effector WipB is a translocated Ser\/Thr phosphatase that targets the host lysosomal nutrient sensing machinery"],"prefix":"10.1038","volume":"7","author":[{"given":"Marie S.","family":"Prevost","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikos","family":"Pinotsis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maud","family":"Dumoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Hayward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Waksman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,25]]},"reference":[{"key":"10249_CR1","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1056\/NEJM197712012972202","volume":"297","author":"JE McDade","year":"1977","unstructured":"McDade, J. E. et al. Legionnaires\u2019 Disease. N. Engl. J. Med. \n                           297, 1197\u20131203 (1977).","journal-title":"N. Engl. J. Med."},{"key":"10249_CR2","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1111\/cmi.12450","volume":"17","author":"I Finsel","year":"2015","unstructured":"Finsel, I. & Hilbi, H. Formation of a pathogen vacuole according to Legionella pneumophila: how to kill one bird with many stones. Cell. Microbiol. \n                           17, 935\u2013950 (2015).","journal-title":"Cell. Microbiol."},{"key":"10249_CR3","doi-asserted-by":"publisher","first-page":"1669","DOI":"10.1073\/pnas.95.4.1669","volume":"95","author":"G Segal","year":"1998","unstructured":"Segal, G., Purcell, M. & Shuman, H. A. Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA \n                           95, 1669\u20131674 (1998).","journal-title":"Proc. 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Multiple substrates of the Legionella pneumophila Dot\/Icm system identified by interbacterial protein transfer. Proc. Natl. Acad. Sci. USA \n                           101, 841\u2013846 (2004).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10249_CR7","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1111\/j.1365-2958.2005.04595.x","volume":"56","author":"EM Campodonico","year":"2005","unstructured":"Campodonico, E. M., Chesnel, L. & Roy, C. R. A yeast genetic system for the identification and characterization of substrate proteins transferred into host cells by the Legionella pneumophila Dot\/Icm system. Mol. Microbiol. \n                           56, 918\u2013933 (2005).","journal-title":"Mol. Microbiol."},{"key":"10249_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000508","volume":"5","author":"D Burstein","year":"2009","unstructured":"Burstein, D. et al. 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Nat Meth \n                           9, 676\u2013682 (2012).","journal-title":"Nat Meth"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10249-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10249-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10249-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T09:27:50Z","timestamp":1671787670000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-017-10249-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,25]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["10249"],"URL":"https:\/\/doi.org\/10.1038\/s41598-017-10249-6","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,25]]},"assertion":[{"value":"16 June 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 August 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 August 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"9450"}}