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Contaminant taxa were removed using a linear regression model using taxa abundances in negative controls as predictor of taxa abundances in subject samples. Both cultured cell counts and sequencing indicated low microbial biomass on the ocular surface. No cultured species was found in all subjects at all times or in all subjects at any one time. After removal of contaminant taxa identified in negative controls using a statistical model, the most commonly detected taxon was <jats:italic>Corynebacterium<\/jats:italic> (11.1%). No taxa were found in all subjects at all times or in all subjects in any one time, but there were 26 taxa present in at least one or more subjects at all times including <jats:italic>Corynebacterium<\/jats:italic> and <jats:italic>Streptococcus<\/jats:italic>. The ocular surface contains a low diversity of microorganisms. Using culture dependent and independent methods, the ocular surface does not appear to support a substantial core microbiome. However, consistently present taxa could be observed within individuals suggesting the possibility of individual-specific core microbiomes.<\/jats:p>","DOI":"10.1038\/s41598-017-10494-9","type":"journal-article","created":{"date-parts":[[2017,8,23]],"date-time":"2017-08-23T14:39:36Z","timestamp":1503499176000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":216,"title":["Temporal Stability and Composition of the Ocular Surface Microbiome"],"prefix":"10.1038","volume":"7","author":[{"given":"Jerome","family":"Ozkan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Diez-Vives","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minas","family":"Coroneo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9557-3001","authenticated-orcid":false,"given":"Torsten","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Willcox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,29]]},"reference":[{"key":"10494_CR1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.exer.2013.07.014","volume":"117","author":"AM McDermott","year":"2013","unstructured":"McDermott, A. 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