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A critical step for the establishment of a successful <jats:italic>Brucella<\/jats:italic> infection is its ability to survive within macrophages. To further understand the mechanisms that <jats:italic>Brucella<\/jats:italic> utilizes to adapt to an intracellular lifestyle, a differential proteomic study was performed for the identification of intracellular modulated proteins. Our results demonstrated that at 48\u2009hours post-infection <jats:italic>Brucella<\/jats:italic> adjusts its metabolism in order to survive intracellularly by modulating central carbon metabolism. Remarkably, low iron concentration is likely the dominant trigger for reprogramming the protein expression profile. Up-regulation of proteins dedicated to reduce the concentration of reactive oxygen species, protein chaperones that prevent misfolding of proteins, and proteases that degrade toxic protein aggregates, suggest that <jats:italic>Brucella<\/jats:italic> protects itself from damage likely due to oxidative burst. This proteomic analysis of <jats:italic>B<\/jats:italic>. <jats:italic>abortus<\/jats:italic> provides novel insights into the mechanisms utilized by <jats:italic>Brucella<\/jats:italic> to establish an intracellular persistent infection and will aid in the development of new control strategies and novel targets for antimicrobial therapy.<\/jats:p>","DOI":"10.1038\/s41598-017-11283-0","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T10:22:57Z","timestamp":1504174977000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Iron-dependent reconfiguration of the proteome underlies the intracellular lifestyle of Brucella abortus"],"prefix":"10.1038","volume":"7","author":[{"given":"M. S.","family":"Roset","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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